PubMed Health⌕ Search

Biomedical subjects

G Heidl

Publications and source records attributed to G Heidl.

At least 37 records · Page 2Linked to original sources
PubMed1983

Human lymphocyte response to CEA: titration of different CEA samples and neutralization experiments with monoclonal anti-CEA antibodies.

By means of the macrophage electrophoretic mobility technique a striking digestive system cancer-associated human lymphocyte response to CEA has been found during a large-scale study including tests in 499 individuals. The question to be answered by this study was whether this response is really CEA-specific. Titration experiments with 3 different CEA preparations in lymphocytes from 5 colorectal cancer patients showed that the threshold dose of CEA necessary to induce lymphocyte responses amounts to 50-100 ng CEA per ml and 10(6) lymphocytes, regardless of the CEA origin and its state of purity. The CEA specificity of the responses was proved by neutralization experiments with 3 CEA-specific monoclonal antibodies. When allowed to react with CEA before lymphocyte incubation, the MABs prevented CEA from inducing lymphocyte responses. Appropriate murine control myeloma protein did not influence these responses. The reactivity of these lymphocyte samples to a teratocarcinoma extract could not be prevented by treating this material with CEA-specific MABs before incubation. Preliminary attempts to enrich the lymphokine(s) released after CEA stimulation resulted in recovery of the activity within 2 arbitrarily cut Sephadex G-100 fractions comprising the mol. wt range of 3000-47,000.

Antibodies, Monoclonal↗
PubMed1983

The value of various methods for determining lymphatic metastases in prostatic carcinoma.

A series of 102 prostatic carcinoma patients was subjected to en bloc pelvic lymphadenectomy. The lymph nodes were collected in groups and examined with the help of the serial section method. An exact N determination is possible by means of the size and location of the lymph node metastases. Compared with this method, the following procedures were critically evaluated: pedal lymphography, excretory urography, clinical examination, explorative lymphadenectomy and pelvic vasography. The results of computer tomography, lymph node biopsy, prostatic lymphography and lymphoscintigraphy are indirectly evaluated. The invasiveness of en bloc lymphadenectomy is discussed.

Carcinoma↗
PubMed1983

[The significance of pelvic lymph-node status in prostatic cancer].

The formation of metastases of carcinoma of the prostate has become more significant since the introduction of en-bloc pelvic lymphadenectomy using the serial-cut method. Lymphogenous evacuation of the pelvis occurs increasingly with advanced and dedifferentiated primary tumours. However, it can be found with low categories of tumours and differentiated carcinomas. pLA is superior to all other procedures for N-determination. If suitably indicated it allows a localized tumour process to be confirmed. Lymphatic oedemas are the most frequent complications. These can be largely avoided by retention of lacunary lymph nodes or primary fitting of lympho-nodo-venous anastomoses.

Humans↗
PubMed1982

Sensitization of human lymphocytes to carcinoembryonic antigen (CEA): neutralization experiments with anti-CEA sera.

By means of the macrophage electrophoretic mobility technique we could show lymphocytes of patients suffering from cancers of the digestive system to be sensitized to carcinoembryonic antigen (CEA). These findings conflict with the common view that CEA is not immunogenic in humans. The aim of the present study was to look as to whether conventional anti-CEA sera can neutralize the activity of a CEA preparation which is responsible for the human lymphocyte response. When 60 ng CEA were preincubated with highly diluted anti-CEA serum and the resulting immune complexes were thereafter co-precipitated by protein A-sepharose, positive lymphocyte responses could no longer be obtained. This effect was observed with 3 anti-CEA sera in 3 cancer patients (colon cancer, stomach cancer, teratocarcinoma), who's lymphocytes responded to CEA by lymphokine release. Normal serum had no neutralizing effect. The anti-CEA sera did not influence the activity of another tumour-relevant extract (teratocarcinoma-derived), to which cancer patients' lymphocytes reacted regardless of the tumour site. The lymphocytes from an oesophagus carcinoma patient, though reacting to the teratocarcinoma preparation, did not respond to CEA, thus, logically, all other tests with normal serum and anti-CEA sera were negative, too. The results show that the digest system cancer-associated lymphocyte reactivity to CEA can be abrogated by conventional anti-CEA sera, which finding indicates that there exist closely CEA-associated "tumour-specific" antigenicities.

Animals↗
PubMed1982

Radioimmunological characterization of carcinoembryonic antigen (CEA) preparations.

A multi-step radioimmunological test system was applied to the characterization of carcinoembryonic antigen (CEA) preparations obtained by column chromatography including ion exchange procedure. CEA contents were estimated by means of a sequential inhibition radioimmunoassay using Protein A-bearing Staphylococci for coprecipitation. Data were expressed as "units CEA" and compared with results obtained from commercial CEA-RIA kits. In addition, attempts were made to evaluate the presence of normal cross-reacting antigens in CEA preparations by the application of an antiserum to perchloric acid extract from normal lung tissue. Data from these tests were expressed as "units NLA" (NLA stands for "normal cross-reacting lung antigen"). The factor "units CEA/units NLA" proved useful as an indication of the tumour specificity of those antigenic components measured in the inhibition assay. Moreover, radioimmunoprecipitation tests with subsequent SDS polyacrylamide gel electrophoretic analysis were performed in order to get information on the molecular weight of the molecule(s) involved in the radioimmunological CEA determination procedure. The test system displayed 1) highly specific CEA reagibility in the standard inhibition assay, 2) test sensitivity of about 200 pg CEA (when related to the Hoffman/La Roche test) and 1 ng CEA (when related to CEA-IRE-SORIN), 3) low reagibility to normal cross-reacting antigens, also when compared to the commercial tests, 4) a molecular weight of about 200,000 daltons for the component(s) measured, and 5) evidence of inhomogeneity of the CEA batches investigated and those international references available. The latter might in part be attributed to so-called species-specific CEA-related determinants. The test system proved also useful for CEA estimations in crude tissue extracts.

Animals↗
PubMed1982

Demonstration of carcinoembryonic antigen (CEA) by means of the immunoperoxidase technique in paraffin-embedded specimens of tumour tissues.

The unlabelled antibody-enzyme method with the peroxidase-antiperoxidase complex (PAP-complex) was used to demonstrate carcinoembryonic antigen (CEA) or CEA-like substances in paraffin-embedded specimens of tumour tissues with an anti-CEA-antiserum of a rabbit. The anti-CEA-antiserum used in this study exhibited a high anti-CEA-activity and a low activity to an impurity. The paraffin-embedded specimens of tumour tissues were investigated at serum dilutions of 1 : 16, 1 : 64, and 1 : 256. The sections of colorectal cancer showed the highest content of CEA. In 36 of 37 cases the tumour tissue reacted to the anti-CEA-antiserum up to a dilution of 1 : 256. The stomach cancer tissues contained significantly lower CEA-levels than the colorectal cancer. The sarcomas, malignant melanomas, the tumour of testis, the prostatic cancers and basal cell carcinomas of the skin were found to contain none or very low CEA-content. In most specimens of these tumours the CEA was not demonstrable at a 1 : 16 dilution of the primary antiserum.

Adenocarcinoma↗
PubMed1982

[Whipple's disease].

With regard to three cases, one of them relapsing after four years, a short survey on Whipples's disease is given. The 3 male patients of medium age suffered from malabsorption and foregoing arthralgia. These symptoms are typical. In most cases first of all chronic infectious or neoplastic diseases are taken into consideration. The finding of PAS-positive macrophages in biopsies of the duodenal mucosa is key to the diagnosis, allowing the successful antibiotic therapy, with preference to oxytetracyline.

Anti-Bacterial Agents↗
PubMed1982

[Lymphogenic metastases in prostatic cancer--operative and histopathologic investigations ].

The necessary application of a differentiated therapy for prostatic cancer requires the involvement of the N-category for determining the therapy. Hitherto existing statements on the lymphogenic metastases of this tumor have been made by means of method whose statements have been limited by the non-presentability of the pelvic lymph nodes, inflammatory-degeneratively changed lymph nodes, and micrometastases. The pelvic en-bloc-lymphadenectomy has been performed and the lymph nodes prepared in successive cuts in the light of a functional patient series of 93 prostatic cancer patients. The standardized procedure allowed statements on the distribution of the pelvic lymph nodes and the corresponding metastases, the size of the metastases and their histologic structure. In prostatic cancer is a high percentage of micrometastases. Lymphogenic metastases can already be detected in low tumor categories, on the other hand metastases are not obligatory in advanced tumors. There is no correlation between the histologic degrees of differentiation of the primary tumor and the lymphogenic metastases. Compared with the metastasis histology the primary tumor histology reveals a distinct morphologic change. According to present investigations, the lymphogenic metastases occur prior to the bone metastases. Since pelvic lymphogenic metastases cannot be covered exactly by means of the conventional methods as well as the metastases cannot be estimated in the single case, there is the necessity for making the pelvic en-bloc-lymphadenectomy before performing a localized tumor treatment such as prostatic vesiculectomy or tele cobalt therapy.

Humans↗
PubMed1981

Preliminary characterization of a colon tumour tissue preparation used in macrophage electrophoretic mobility (MEM) test1.

A 3M KC1-extract on nine samples of colon carcinomas was freed from human serum proteins by immunoadsorption. Both the KC1-extract and the immunoadsorbed preparation of this KC1-extract were used in MEM test and gave characteristic reaction patterns. The two preparations were compared and preliminarily characterized by immunological and electrophoretical methods.

Antigens, Neoplasm↗
PubMed1981

[Value of lymphography in prostatic cancer - comparison with pelvic en-bloc lymphadenectomy].

On the basis of 62 pelvic en bloc lymphadenectomies in the carcinoma of the prostate the lymphogenic formation of metastases is histologically determined. In 41 patients the comparison between lymphographic and histological N-determination is performed. The security of the lymphography in the carcinoma of the prostate in the lymphographically establishable pelvic region is recognized with 70%. By the pelvic en bloc lymphadenectomy in 50% of the cases a change of the preoperatively established N-determination takes place. The pedal lymphography is overestimated as method in the carcinoma of the prostate. As a secure technique in the determination of the lymphogenic formation of metastases at present the pelvic en bloc lymphadenomectomy with histological elaboration of the preparations is to be regarded. To this methods belongs a decisive diagnostic evidence in the determination of the formation of metastases of the carcinoma of the prostate by pelvic lymph nodes. The value of the lymphography consists in the simultaneous establishment of aortic metastases. Apart from this an intraoperative X-ray control may partly prove the radicality of the intervention.

Humans↗
PubMed1981

[Fatal complication of a combined coarctation of the aortic arch and isthmus with anomalies of the aortic valve (author's transl)].

It is reported on a combined coarctation of the aortic arch and isthmus with anomalies of the aortic valve and an aortic sinus aneurysm in case of a 17-year-old woman died of rupture of ascending aorta. By means of histological investigations of aortic tissue taken from aortic coarctation and other areas of aorta it is tried to give a pathognomonic declaration of these anomalies. Findings are allocated to classifications for coarctation of the aorta. Moreover, the pathological findings are subject for comparison with analogous cases mentioned in literature, especially with regard to their frequency, origin, complications, and the age reached.

Adolescent↗
PubMed1979

[Lymphogenic metastasis in prostate carcinoma and radiation therapy].

Our examinations allow the following conclusions: 1. Before the beginning of a curative radiotherapy of the carcinoma of the prostate gland in a negative and questionably positive lymphogramme the pelvic lymphadenectomy with histologic examination of the lymph nodes is indicated. 2. In the negative findings of the histologically examined lymph nodes an exclusive local tumour irradiation is justified. 3. If in regional and negative findings micrometastases appear in the area of juxtaregional lymph nodes the additional curative irradiation of the pelvic lymph nodes seems to be indicated for the comprehension of the regional lymph nodes. 4. When there are micrometastases in the area of the aa. iliacae communes or macroscopically extended metastases in the regional and juxtaregional lymph area, so we regard as a rule a radiotherapy of the carcinoma of the prostate gland no more indicated and recommend a contrasexual treatment. The necessary expansion of the radiotherapy is according to our hitherto existing experiences badly tolerated by the for the most part older patients. 5. The intraoperative labelling of the prostate gland and the determination of the size of the prostate gland facilitate the localisation of the tumour for the radiotherapist.

Carcinoma↗
PubMed1978

[Hemangioendothelioma--a case contribution].

By means of two casuistics the haemangioendothelioma is demonstrated. The malignant tumour may occur in all tissues and organs metastasizing in haematogenic and lymphogenic manners. The thorotrast or vinylchloride-caused promotion of the tumour formation is especially discussed because of its considerably prophylactic importance.

Adrenal Gland Neoplasms↗
PubMed1977

Immunological tumour profile: organ-specific carcinoma diagnosis in patients employing the macrophage electrophoretic mobility test.

The macrophage electrophoretic mobility (MEM) test provides a highly sensitive in vitro technique for the detection of cell-mediated immunity in man. The principle involved is the lymphokine-mediated reduction of the negative surface charge of guinea pig macrophages shown by the slowing of the macrophages during cell electrophoresis. Lymphocytes from 162 patients were tested by MEM. They were exposed to a battery of KCl extracts from normal and malignant human tissues, to encephalitogenic protein (EP), to carcinoembryonic antigen (CEA), and to thyroglobulin. Variable lymphocyte responses to EP, CEA and KCl extracts from different cancers gave MEM reaction profiles common to patients with carcinomas of the same organ origin.

Antigens, Neoplasm↗
PubMed1976

[Studies on the immunologic specificity of the macrophage-electrophoresis mobility test in mice with syngeneic and allogeneic transplants of mammary carcinoma].

The study was performed to answer the question as to whether the macrophage electrophoretic mobility test (MEM-test) is suitable to detect allogeneic and syngeneic lymphocyte sensitization in the murine mammary tumour host system. Lymph node cells of both CBAf and XVII/Bln mice injected with allogeneic or syngeneic mammary tumour cells reacted specifically to hypertonic KCl extracts from allogeneic or syngeneic mammary tumours. Lymph node cells of nontreated control mice did not react at all. The MEM results reflect lymphocyte sensitizations which are specific with respect to the claim of transplantation and tumour immunology in the system employed.

Animals↗