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Biomedical subjects

G Holzer

Publications and source records attributed to G Holzer.

54 records · Page 3Linked to original sources

Quantitative evaluation of functional capacity during isoniazid therapy in Huntington's disease.

Eleven patients with Huntington's disease were treated with high doses of isoniazid. In addition to clinical assessment, the functional capacity was evaluated quantitatively. The symptoms of four of the patients showed a marked improvement and their functional capacity increased. The condition of one patient with the rigid form (Westphal's variant) deteriorated, and the remaining six patients showed no change. Patients with prominent mental disturbances appeared to respond best to isoniazid therapy.

Adult↗

The contribution of cometary volatiles to the primitive Earth.

It has been estimated that during its early history the Earth captured a mass of cometary material of the order of 10(23) grams. Since carbon is supposed to be at least three times more abundant in comets than in carbonaceous chondrites (3.5% C in C 1 chondrites), it can be deduced that about 1 x 10(22) grams of carbon (as carbon compounds), was added by comets to the surface of the prebiotic Earth. This carbon value is of the same order of magnitude as the value of the organic carbon buried in the Earth's sedimentary shell, but approximately one order of magnitude lower than the Earth's surface total carbon (7 x 10(22) gm). The capture of comets by the Earth would also have contributed to generating the appropriate aqueous and reducing environmental conditions necessary for organic synthesis. Although it is possible that some of the cometary carbon compounds falling on the Earth survived, most of them were probably decomposed by the heat and shock waves of the cometary collision. Upon quenching to low temperatures, however, the reactive chemical species produced by the impact would have recombined, leading to the synthesis of a great variety of organic molecules. Laboratory experiments with radiation, heat and shock waves have demonstrated that some of the synthesized compounds are biochemical molecules: amino acids, sugars, purines, and pyrimidines. These are essential to all living systems.

Carbon↗

Lipids as a principle for the identification of archaebacteria.

The 'Archaebacteria' consist of several distinct subgroups including methanogens, extreme halophiles and specific thermoacidophiles. These bacteria are distinct from other bacteria with respect to their characteristic RNA compositions, the absence of muramic acid in the cell walls and the predominance of nonsaponifiable [correction of nonsaponifable] lipids. The lipid composition of the archaebacteria consists of isoprenoid and hydroisoprenoid hydrocarbons and isopranyl glycerol ether lipids. The pathways for the biosynthesis of the lipid components are those shared by most microorganisms and demonstrate a close relationship; however, an independent line of descent is indicated by the formation of the isopranyl glycerol ether lipids. This discontinuity formulates a point for delineating the early stages of biological evolution and for dividing bacteria into two subgroups.

Archaea↗

Capillary column gas chromatographic profile analysis of volatile compounds in sera of normal and virus-infected patients.

Using a transevaporator sampling technique, the volatile profiles from 70 microliter of serum were obtained by capillary-column gas chromatography. The complex chromatograms were interpreted by a combination of manual and computer techniques and a two-peak ratio method devised for the classification of normal and virus-infected sera. Using the K-nearest neighbor approach 85.7% of the unknown samples were classified correctly. Some preliminary results indicate the possible use of the method for the assessment of virus susceptibility.

Chromatography, Gas↗

Squalenes, phytanes and other isoprenoids as major neutral lipids of methanogenic and thermoacidophilic "archaebacteria".

The neutral lipids of nine species of methanogenic bacteria including five methanobacilli, two methanococci, a methanospirillum, one methanosarcina as well as two thermoacidophilic bacteria, Thermoplasma and Sulfolobus, were analyzed. The major components were C30, C25 and/or C20 acylic isoprenoid hydrocarbons with a continuous range of hydroisoprenoid homologues. The range of acyclic isoprenoids detected were from C14 to C30. Apart from Methanosarcina barkeri, squalene and/or hydrosqualene derivetives were the predominant components in all species studied. The components of Methanosarcina barkeri were a family of C25 homologues. The distribution of the neutral lipid components and their specific variations in relative intensities emphasized the differences between the test organisms while the generic nature of the isoprenoid hydrocarbons demonstrated similarities between the diverse bacteria. The neutral lipid compositions from these bacteria, many of which exist in environmental conditions like those described for the various evolutionary stages of the archean ecology, resemble the isoprenoid distribution isolated from ancient sediments and petroleum. Therefore, these finding may have major implications to biological and biogeochemical evolution.

Cell Wall↗

The photolytic degradation and oxidation of organic compounds under simulated Martian conditions.

Cosmochemical considerations suggest various potential sources for the accumulation of organic matter on Mars. However the Viking Molecular Analysis did not indicate any indigenous organic compounds on the surface of Mars. Their disappearance from the top layer is most likely caused by the combined action of the high solar radiation flux and various oxidizing species in the substances and a sample of the Murchison meteorite was tested under simulated Martian conditions. After adsorption on powdered quartz, samples of adenine, glycine and naphthalene were irradiated with UV light at various oxygen concentrations and exposure times. In the absence of oxygen, adenine and glycine appeared stable over the given irradiation period, whereas a definite loss was observed in the case of naphthalene, as well as in the volatilizable and pyrozable content of the Murchison meteroite. The presence of oxygen during UV exposure caused a significant increase in the degradation rate of all samples. It is likely that similar processes have led to the destruction of organic materials on the surface of Mars.

Adenine↗

The organic composition of the Allan Hills carbonaceous chondrite (77306) as determined by pyrolysis-gas chromatography-mass spectrometry and other methods.

The amino acid composition and the pyrolyzable organic content of the Antarctic Allan Hills meteorite (ALHA 77306.9 and ALHA 77306.17) was examined. The pyrolysis products are very similar to those of the Murchison meteorite. The identified amino acids are present at quite low levels, they are racemic and are structurally similar to those observed in the Murchison meteorite. This indicates the possible chemical synthesis of these compounds in the meteorite parent body. The Allan Hills meteorite is not detectably contaminated.

Amino Acids↗

The effects of ultraviolet light on the degradation of organic compounds: a possible explanation for the absence of organic matter on Mars.

The analysis of the top layer of the Martian regolith at the two Viking landing sites did not reveal any indigenous organic compounds. However the existence of such compounds at deeper layers cannot be ruled out. Cosmochemical considerations indicate various potential sources for organic matter on Mars, such as comets and meteorites. Its disappearance from the top layer could be caused by degradation processes on the surface of the planet. Possible destructive agents include ultraviolet light, oxygen and metal oxides. In this study we tested the stability of a sample of the Murchison meteorite and various organic substances which have been detected in carbonaceous chondrites, such as glycine, adenine and naphthalene, to the action of ultraviolet light. The compounds were adsorbed on powdered quartz and on California desert soil and were irradiated in the presence or absence of oxygen. The organic content, before and after irradiation, was measured by carbon elementary analysis, UV-absorption, amino acid analysis or pyrolysis-gas chromatography-mass spectrometry. In the absence of oxygen, adenine and glycine appear to be stable over the given period of irradiation. A definite degradation was noticed in the case of naphthalene and the Murchison meteorite. In the presence of oxygen in amounts comparable to those on Mars all compounds were degraded. The degree of degradation was influenced by the irradiation time, temperature and oxygen content.

Adenine↗

Gas chromatographic-mass spectrometric evaluation of exhaled tobacco smoke.

The impact of cigarette smoking on the distribution of organic substances in ambient air has been determined for the intermediate volatility range. A simple sampling procedure was employed, involving gas-solid adsorption onto an organic polymer followed by direct thermal elution onto a glass capillary column. Aliphatic and substituted aromatic hydrocarbons are predominant in urban atmospheres. Depending on location and weather conditions the total concentration of such volatiles can differ by as much as a factor of 20. This high background variation makes it difficult to analyze for trace substances with low odor threshold values, such as encountered in cigarette smoke. Standard cigarettes were smoked in a relatively small room, having no air filtration system. Air samples of approximately 3.51 were taken. The amount of volatiles added to air by cigarette smoking is unsignificant. Substances were analyzed and identified by gas chromatography and gas chromatography-mass spectrometry with glass capillary columns. Many compounds reported in cigarette smoke condensate have been confirmed.

Air Pollutants↗

Trace analysis of organic volatiles in water by gas chromatography-mass spectrometry with glass capillary columns.

Traces of volatile organic materials in water have been concentrated by gas phase stripping and adsorption onto a porous polymer. A simple all-glass sampling device is proposed which allows efficient concentration at elevated temperatures. Sample transfer from absorbent into a gas chromatographic column is effected by a simple one-step procedure involving heat desorption. The capacity of the absorbent has been determined for a number of model substances which are found in water. Under the sampling conditions used, compounds being less volatile than benzene are usually quantitatively retained, with some exceptions. Separations were effected with highly efficient glass capillary columns. Water samples, collected from a small number of locations, included both tap water and untreated waters. A number of volatiles have been determined by gas chromatography-mass spectrometry in drinking water and in a river which flows through an industrialized area. The drinking water examined contains a large number of chlorinated and brominated compounds whereas the river water is largely free from this class of substances. Derivatives of camphor and terpenes have been identified in this particular river.

Absorption↗

Tick-borne encephalitis in the Saarland and the Rhineland-Palatinate.

The Saarland and the Rhineland-Palatinate are not considered endemic regions for tick-borne encephalitis (TBE), and patients in this region have not been routinely advised to undergo vaccination or serologic testing for TBE. In 1994, a significantly increased incidence of TBE cases was noted in the neighbouring state of Baden-Württemberg. In the same year, the first TBE acquired in the Saarland was diagnosed. To investigate the infection risk for TBE in the Saarland and Rhineland-Palatinate, the records of 2,123 serologic tests for TBE collected since 1989 were systematically examined. In addition, 904 frozen sera of patients displaying inflammatory changes in the cerebrospinal fluid (CSF) were analyzed. IgG and IgM antibodies against TBE virus were found in 15 patients, four of which were verified clinically and serologically as TBE. One of these four cases was certainly and another was probably acquired in the Saarland. Three other patients displayed serologic signs of a TBE virus contact. The results of this study suggest that the occurrence of single cases in the Saarland has to be considered, but the risk is very small.

Adult↗

Serum concentrations of APO-1/Fas and interleukin-1beta-converting enzyme in osteosarcoma correlate with response to chemotherapy.

BACKGROUND: Cytotoxic drugs used in neoadjuvant chemotherapy may exert their effect by activation of apoptosis. Both APO-1/Fas and Interleukin-1beta-converting (ICE)/caspase-1 are believed to act as mediators of apoptotic cell death. PATIENTS AND METHODS: The sera of 21 patients with high-grade osteosarcoma were tested at the time of diagnosis by ELISA and compared with clinical data. RESULTS: The mean APO-1 serum levels were 844 +/- 344 pg ml(-1) and the mean ICE serum levels were 212 pg ml(-1) +/- 155 pg ml(-1) for all patients. Furthermore, both serum levels were found to be statistically significantly higher in patients with good regression grades compared to those in patients with bad regression grades. CONCLUSION: In highly-malignant osteosarcoma elevated serum levels of both APO-1 and ICE seem to be predictive of good response to chemotherapy.

Adolescent↗

Serum levels of TNF-beta and sTNF-R in patients with malignant bone tumours.

OBJECTIVE: To determine serum levels of TNF-beta and soluble TNF-R in patients with primary highly malignant bone tumours. PATIENTS AND METHODS: Sera of 27 patients with highly malignant osteosarcoma and Ewing sarcoma were taken at the time of diagnosis and analysed by ELISA. RESULTS: Both TNF-beta and sTNF-R levels were lower in sera from osteosarcoma patients as compared to those from Ewing sarcoma. In patients with high-grade osteosarcoma, but not Ewing sarcoma, high levels of TNF-beta correlated with bad response to neoadjuvant chemotherapy. CONCLUSION: In patients with high-grade osteosarcoma TNF-beta levels seem to be of predictive value and both TNF-beta and sTNF-R seem to be of diagnostic value for differentiation between high-grade osteosarcoma and Ewing sarcoma.

Adolescent↗