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G Horneck

Publications and source records attributed to G Horneck.

At least 55 records · Page 3Linked to original sources
PubMed1996

Biological dosimetry of solar radiation for different simulated ozone column thicknesses.

During the Spacelab mission D-2, in the experiment RD-UVRAD, precalibrated biofilms consisting of dry monolayers of immobilised spores of Bacillus subtilis (strain Marburg) were exposed, for defined intervals, to extraterrestrial solar radiation filtered through an optical filtering system, to simulate different ozone column thicknesses. After the mission, the biofilms were processed and optical densities indicative of any biological activity were determined for each exposure condition by image analysis. For the different simulated ozone column thicknesses, biologically effective irradiances were experimentally determined from the biofilm data and compared with calculated data using a radiative transfer model and the known biofilm action spectrum. The data show a strong increase in biologically effective solar UV irradiance with decreasing (simulated) ozone concentrations. The full spectrum of extraterrestrial solar radiation leads to an increment of the biologically effective irradiance by nearly three orders of magnitude compared with the solar spectrum at the surface of the Earth for average total ozone columns.

Bacillus subtilis↗
PubMed1995

Results of space experiments.

Life science research in space was started in Europe with the first Biostack experiment flown onboard Apollo 16 in 1972. Biostack was designed to investigate the biological effects of single heavy ions of cosmic radiation. Among several undertakings towards this goal, the Biostack achieved the highest precision in the determination of the spatial correlation of the observed biological response of single test organisms to the passage of single heavy ions, which is the mandatory requirement. It also provided information on the influence of additional spaceflight factors, such as microgravity, on radiation effects and measurements of the spectrum of charge and energy of the cosmic radiation. The experiment was performed as an international cooperation effort. This report gives a summary of the biological data accumulated in this and the follow-on experiments of the Biostack program.

Animals↗
PubMed1995

Biological responses to space: results of the experiment "Exobiological Unit" of ERA on EURECA I.

Spores of different strains of Bacillus subtilis and the Escherichia coli plasmid pUC19 were exposed to selected conditions of space (space vacuum and/or defined wavebands and intensities of solar ultraviolet radiation) in the experiment ER 161 "Exobiological Unit" of the Exobiology Radiation Assembly (ERA) on board of the European Retrievable Carrier (EURECA). After the approximately 11 months lasting mission, their responses were studied in terms of survival, mutagenesis in the his (B. subtilis) or lac locus (pUC19), induction of DNA strand breaks, efficiency of DNA repair systems, and the role of external protective agents. The data were compared with those of a simultaneously running ground control experiment. The survival of spores treated with the vacuum of space, however shielded against solar radiation, is substantially increased, if they are exposed in multilayers and/or in the presence of glucose as protective, whereas all spores in "artificial meteorites", i.e. embedded in clays or simulated Martian soil, are killed. Vacuum treatment leads to an increase of mutation frequency in spores, but not in plasmid DNA. Extraterrestrial solar ultraviolet radiation is mutagenic, induces strand breaks in the DNA and reduces survival substantially; however, even at the highest fluences, i.e. 3 x 10(8) J m-2, a small but significant fraction of spores survives the insolation. Action spectroscopy confirms results of previous space experiments of a synergistic action of space vacuum and solar UV radiation with DNA being the critical target.

Bacillus subtilis↗
PubMed1995

Effects of vacuum UV and UVC radiation on dry E. coli plasmid pUC19. I. Inactivation, lacZ- mutation induction and strand breaks.

Using Escherichia coli plasmid pUC19 as a test system to study the effects of radiation on DNA at the molecular level, the wavelength (160-254 nm) dependence of inactivation (loss of the ability to transform E. coli), mutation induction in the target gene lacZ and induction of single-strand breaks and double-strand breaks was investigated. The same fluences were applied for all endpoints tested. In the UVC range, the cross-sections of inactivation and mutation induction match the DNA absorption curve, whereas the cross-section for single-strand break induction deviates from the DNA curve, especially at 220 nm. In the vacuum UV range, with increasing energy of the photons, the cross-sections of inactivation and single-strand breaks increase sharply (from 190 to 160 nm by more than one order of magnitude), which is not reflected by the DNA curve. In this UV range, the shape of the action spectrum is similar to that of the absorption curve of the sugar phosphate moiety of DNA. Only after irradiation with vacuum UV at 160 nm are double-strand breaks detected. Their induction rate is about one order of magnitude lower than that of single-strand breaks at the same wavelength; however, their induction rate is at least twice that of single-strand breaks at longer wavelengths. Concerning mutation induction, the increment in the vacuum UV range is less well expressed. The data suggest the contribution of different kinds of photochemical injury to inactivation and mutation induction.

Dose-Response Relationship, Radiation↗
PubMed1995

Effects of vacuum UV and UVC radiation on dry Escherichia coli plasmid pUC19. II. Mutational specificity at the lacZ gene.

The mutational spectra at the lacZ gene, induced either by vacuum UV at 160 nm or UVC at 254 nm in vacuum-dried preparations of Escherichia coli plasmid pUC19 DNA, have been characterized from 72 E. coli-propagated mutants by DNA sequencing. In plasmids irradiated in vacuum, vacuum UV is five times more mutagenic than UVC. In the UV-induced mutants, base substitutions largely predominate, with GC-->AT (G, guanine; C, cytosine; A, adenine; T, thymine) transitions being the most abundant type of base change for vacuum UV (61%) and UVC (47%). Most of the GC-->AT transitions appear to occur at dipyrimidine sites, which are located at the non-transcribed DNA strand. Some, but not all, hot spots for GC-->AT transitions are identical for vacuum UV and UVC. Frameshifts, resulting from a loss of the thymine residue, are specific for UVC (22%), and were not detected after treatment with vacuum UV. They occur predominantly at thymine runs of the transcribed DNA strand. Only a few deletions were detected following irradiation with vacuum UV (7.5%) and UVC (2%); however, their frequency is not enhanced compared with the spontaneous mutation spectrum. The data confirm the important role of base substitution mutations in UV-induced mutagenesis, which is not only valid for the UVC range, but extends towards the vacuum UV range.

Base Sequence↗
PubMed1995

Planetary protection considerations for MarsNet and Mars sample return missions.

The ESA MarsNet mission proposal consists most probably of a trio of Mars landers. These landers each contain a variety of scientific equipment. The network of stations demands for a definition of its planetary protection requirements. With respect to the MarsNet mission only forward contamination problems will be considered. Future involvement of European efforts in planetary exploration including sample returns will also raise the problem of back contamination. A tradeoff study for the overall scientific benefit with respect to the approximative cost is necessary. Planetary protection guide-lines will be proposed by an interdisciplinary and international board of experts working in the fields of both biology and planetary science. These guide-lines will have to be flexible in order to be modified with respect to new research results, e.g. on adaptation of microorganisms to extreme (space) conditions. Experiments on the survival of microorganisms at conditions of simulated Mars surface and subsurface will have to be conducted in order to obtain a baseline data collection as a reference standard for future guide-lines.

Containment of Biohazards↗
PubMed1995

Interpretation of mutation induction by accelerated heavy ions in bacteria.

In this report, a quantitative interpretation of mutation induction cross sections by heavy charged particles in bacterial cells is presented. The approach is based on the calculation of the fraction of energy deposited by indirect hits in the sensitive structure. In these events the particle does not pass through the sensitive volume, but this region is hit by delta rays. Four track structure models, developed by Katz (in Quantitative Mathematical Models in Radiation Biology, pp. 57-83, Springer-Verlag, 1988). Chatterjee et al. (Radiat. Res. 54, 479-494, 1973), Kiefer and Straaten (Phys. Med. Biol. 31, 1201-1209, 1982) and Kudryashov et al. (Proceedings of the First Soviet Congress on Microdosimetry, Atomizdat, Moscow, 1973), respectively, were used for the calculations. With the latter two models, very good agreement of the calculations with experimental results on mutagenesis in bacteria was obtained. Depending on the linear energy transfer (LET infinity) of the particles, two different modes of mutagenic action of heavy ions are distinguished: "delta-ray mutagenesis," which is related to those radiation qualities that preferentially kill the cells in direct hits (LET infinity > or = 100 keV/microns), and "track core mutagenesis," which arises from direct hits and is observed for lighter ions or ions with high energy (LET infinity < or = 100 keV/microns).

Bacillus subtilis↗
PubMed1994

Mutation induction by accelerated heavy ions in bacteria.

Induction of lacI- forward mutations in Escherichia coli Ymcl and his(-)-->his+ reversions in Salmonella typhimurium TA102 was investigated after irradiation with heavy ions in the range of Z = 1-36. Particle specific energies (E) were in the range of 1-600 MeV/u. A strong dependence of the mutation induction cross-section (sigma m) on both particle energy and LETinfinity was observed. The results suggest that two different ranges of LETinfinity can be distinguished. In the range of high LETinfinity (> 100 keV/micron) sigma m increases with increasing specific particle energy if LETinfinity is kept constant (Fe ions as compared with carbon ions or alpha-particles). In the range of low LETinfinity (< 100 keV/micron) sigma m decreases with increasing energy (Ne ions as compared with He ions).

Deuterium↗
PubMed1994

HZE particle effects in space.

Among the various particulate components of ionizing radiation in space, heavy ions (the so-called HZE particles) have been of special concern to radiobiologists. To understand the ways by which HZE particles of cosmic radiation interact with biological systems, methods have been developed to precisely localize the trajectory of an HZE particle relative to the biological object and to correlate the physical data of the particle with the biological effects observed along its path. In a variety of test systems, injuries were traced back to the traversal of a single HZE particle, such as somatic mutations, and chromosomal aberrations in plant seeds, development disturbances and malformations in insect and salt shrimp embryos, or cell death in bacterial spores. In the latter case, a long-ranging killing effect around the particle's track was observed. Whereas, from spaceflight experiments, substantial infomation has been accumulated on single HZE particle effects in resting systems and in a few embryonic systems, there is a paucity of data on cosmic radiation effects in whole tissues or animals, especially mammalians.

Animals↗
PubMed1994

Long-term survival of bacterial spores in space.

On board of the NASA Long Duration Exposure Facility (LDEF), spores of Bacillus subtilis in monolayers (10(6)/sample) or multilayers (10(8)/sample) were exposed to the space environment for nearly six years and their survival was analyzed after retrieval. The response to space parameters, such as vacuum (10(-6) Pa), solar electromagnetic radiation up to the highly energetic vacuum-ultraviolet range (10(9) J/m2) and/or cosmic radiation (4.8 Gy), was studied and compared to the results of a simultaneously running ground control experiment. If shielded against solar ultraviolet (UV)-radiation, up to 80 % of spores in multilayers survive in space. Solar UV-radiation, being the most deleterious parameter of space, reduces survival by 4 orders of magnitude or more. However, up to 10(4) viable spores were still recovered, even in completely unprotected samples. Substances, such as glucose or buffer salts serve as chemical protectants. With this 6 year study in space, experimental data are provided to the discussion on the likelihood of "Panspermia".

Bacillus subtilis↗
PubMed1994

Double strand breaks in the DNA of Bacillus subtilis cells irradiated by heavy ions.

Cells of Bacillus subtilis strain TKJ 8431 in stationary phase were irradiated with X-rays (150 kV at DLR) or heavy ions (Ne, Ar, Pb with residual energies between 3 and 15 MeV/u at GSI). The action cross section for the formation of double strand breaks in the DNA of the irradiated cells follows a similar dependence on mass and energy of the ions as has been found for various biological endpoints, e.g. inactivation, mutagenesis and repair efficacy.

Argon↗
PubMed1994

Mutagenic effects of heavy ions in bacteria.

Various mutagenic effects by heavy ions were studied in bacteria, irradiated at accelerators in Dubna, Prague, Berkeley or Darmstadt. Endpoints investigated are histidine reversion (B. subtilis, S. typhimurium), azide resistance (B. subtilis), mutation in the lactose operon (E. coli), SOS chromotest (E. coli) and lambda-prophage induction (E. coli). It was found that the cross sections of the different endpoints show a similar dependence on energy. For light ions (Z < or = 4) the cross section decreases with increasing energy. For ions of Z = 10, it is nearly independent of energy. For heavier ions (Z > or = 26) it increases with energy up to a maximum or saturation. The increment becomes steeper with increasing Z. This dependence on energy suggests a "mutagenic belt" inside the track that is restricted to an area where the density of departed energy is low enough not to kill the cell, but high enough to induce mutations.

Azides↗
PubMed1994

Continuous dosimetry of the biologically harmful UV-radiation in Antarctica with the biofilm technique.

For the first time, a continuous biological dosimetry experiment for cytotoxic solar UV-radiation has been performed in Antarctica. The biologically harmful UV-radiation on the ground was measured at the German Antarctic Georg von Neumayer Station (70 degrees 37' S, 80 degrees 22' W) from December 1990 to March 1992 using the biofilm technique. The UV-sensitive targets were dried spores of Bacillus subtilis which were immobilized on the film surface. The UV-induced inhibition of biological activity, determined photometrically from the protein synthesized after incubation and staining, was taken as a measure for the absorbed UV-dose. Films were exposed in horizontal position for time intervals ranging from 4 days during summer up to 51 and 41 days before and after the polar night respectively. The use of different cut-off filters allowed the calculation of the biologically effective UVA, UVB and the complete UV-radiation (UVA + B). The data were compared with the global radiation and the ozone column thickness indicating an increase of biologically harmful UVB radiation during austral spring at reduced ozone concentrations yielding a radiation amplification factor (RAF) of 1.4, whereas for the total UV(A + B) range the RAF amounted to 0.3.

Antarctic Regions↗
PubMed1993

Responses of Bacillus subtilis spores to space environment: results from experiments in space.

Onboard of several spacecrafts (Apollo 16, Spacelab 1, LDEF), spores of Bacillus subtilis were exposed to selected parameters of space, such as space vacuum, different spectral ranges of solar UV-radiation and cosmic rays, applied separately or in combination, and we have studied their survival and genetic changes after retrieval. The spores survive extended periods of time in space--up to several years--, if protected against the high influx of solar UV-radiation. Water desorption caused by the space vacuum leads to structural changes of the DNA; the consequences are an increased mutation frequency and altered photobiological properties of the spores. UV-effects, such as killing and mutagenesis, are augmented, if the spores are in space vacuum during irradiation. Vacuum-specific photoproducts which are different from the 'spore photoproduct' may cause the synergistic response of spores to the simultaneous action of UV and vacuum. The experiments provide an experimental test of certain steps of the panspermia hypothesis.

Bacillus subtilis↗
PubMed1992

Heavy ion induced double strand breaks in bacteria and bacteriophages.

DNA damage induced by heavy ions in bacterial cells and bacteriophages such as Bacillus subtilis, E. coli and Bacteriophage T1 were investigated by analyzing the double strand breaks in the chromosomal DNA. This kind of lesion is considered as one of the main reasons for lethal events. To analyze double strand breaks in long molecules of DNA--up to some Mbp in length--the technique of pulse field agarose gel electrophoresis has been used. This allows the detection of one double strand break per genome. Cell lysis and DNA isolation were performed in small agarose blocks directly. This procedure secured minimum DNA destruction by shearing forces. After running a gel, the DNA was stained with ethidium bromide. The light intensity of ethidium bromide fluorescence for both the outcoming (running) DNA and the remaining intact DNA were measured by scanning. The mean number of double strand breaks was calculated by determining the quotient of these intensities. Strand break induction after heavy ion and X-ray irradiation was compared.

Argon↗
PubMed1992

Thymine photoproduct formation and inactivation of intact spores of Bacillus subtilis irradiated with short wavelength UV (200-300nm) at atmospheric pressure and in vacuo.

Vacuum exposure renders the survival of spores of Bacillus subtilis approximately five times more sensitive to ultraviolet light irradiation than exposure under atmospheric conditions. The photoproduct formation in spores irradiated under ultrahigh vacuum (UHV) conditions is compared to the photoproduct formation in spores irradiated at atmospheric pressure. Compared to irradiation at atmospheric pressure, where only the "spore photoproduct" 5-thyminyl-5,6-dihydrothymine (TDHT) can be detected, two additional photoproducts, known as the c,s and t,s isomers of thymine dimer (T<>T) are produced in vacuo. The spectral efficiencies for photoproduct formation in spores under atmospheric and vacuum conditions are compared. Since there is no increased formation of TDHT after irradiation in vacuum, TDHT cannot be made responsible for the observed vacuum effect. "Vacuum specific" photoproducts may cause a synergistic response of spores to the simultaneous action of ultraviolet light (UV) and UHV. Three different mechanisms are discussed for the enhanced sensitivity of B. subtilis spores to UV radiation in vacuum. The experiments described contribute valuable research information on the chance for survival of microorganisms in outer space.

Bacillus subtilis↗
PubMed1992

Induction of lacI- mutations in Escherichia coli cells after single and split-dose irradiation.

In the lacI system of Escherichia coli, X-ray mutagenesis follows a linear-quadratic curve with suppression; the survival curve is exponential. Dose fractionation leads to nearly complete repair of premutational lesions during an incubation interval of 3.5 h. Repair starts with a delay of 1.5-2 h, suggesting the involvement of an inducible repair/mutation fixation system. The dose-dependence of mutagenesis is described by a simple model assuming two hits being required. A probable explanation might be that the premutagenic lesions consist of two closely spaced lesions on the opposite strands of the DNA molecule.

Dose-Response Relationship, Radiation↗