Biomedical subjects

G Huang

Publications and source records attributed to G Huang.

At least 109 records · Page 6Linked to original sources

PubMed1999

[Significance of maximal midexpiratory time and F-V curve configuration in testing small airway function for smokers].

To evaluate the clinical significance of maximal midexpiratory time (MET) in testing small airway functions for smokers, the routine ventilatory functions, MET and F-V curve configurations were studied in 116 passive and light, heavy active smokers. Routine ventilatory functions and F-V curve configuration were determined with LR80 flow volume recorder. The MET was measured with FJD80 spirometer. The results showed that the distribution of convex type rose with the degree of smoking. In heavy smokers, the MET elongated to 127% of the predicted value and in the convex type F-V curve, it elongated to 120% of the predicted value; both were consistent with the decrease of corresponding V50, V25. This implied when MET elongated to 120%-130% of the predicted value in smokers, there had been slight disturbances in small airway functions. It is therefore suggested that MET and convex type F-V curve both are sensitive indices for testing small airway functions, thus it should be of clinical significance and be appropriate for general application.

Adult↗

PubMed1999

Mechanical and histological analysis of bone-pedicle screw interface in vivo: titanium versus stainless steel.

OBJECTIVE: To investigate the differences in bone interface between titanium and stainless steel pedicle screws in the lumbar spine. METHODS: Eighteen adult mini-pigs that underwent total laminectomy, posterolateral spinal fusion (L4-L5) were randomly selected to receive stainless steel (9) or titanium pedicle screw devices (9). In both groups, the devices were CCD (Sofamore Danek) type with the same size and shape. The postoperative observation time was 3 months. Screws from L4 were harvested along their long axis of pedicle for histomorphometric study. Bone-screw interface and bone volume from thread were examined using linear intercept techniques. Mechanical testing (torsional test and pull-out test) was performed on the screws from L5. RESULTS: The titanium screw group had a significantly higher maximum torque (P < 0.05) and angle related stiffness (P < 0.05) measured by torsional test. In the pull-out tests, no differences were found between the two groups in relation to the maximum load, stiffness and energy to failure. Direct bone contact with the screw in percentage was 29.4% for stainless steel and 43.8% for titanium (P < 0.05). No differences in the bone purchase between the vertebral body part and pedicle part were found. CONCLUSION: Pedicle screws made of titanium have a better bone-screw interface binding than screws made of stainless steel. Torsional tests are more informative for bone-screw interface study. Pull-out tests seem less valuable when comparing bone purchase of screws made from different materials.

Animals↗

PubMed1999

[The relationship between the expression of transferrin receptors (TfR) on leukemia cells and the cell proliferation and iron metabolism status in childhood acute leukemias].

OBJECTIVE: To explore the relationship between transferrin receptor (TfR) expression and cell proliferation capacity and iron metabolism status. METHODS: The binding site numbers and dissociation constant (Kd) of TfR of leukemic cells were assayed by radio-ligand binding assay. RESULTS AND CONCLUSION: The binding site numbers of TfR were (7.826 +/- 6.054) x 10(4) sites/cell in ALL group and (20.406 +/- 17.876) x 10(4) sites/cell in AML group, and the Kds of TfR were (6.468 +/- 4.777) nmol/L and (8.683 +/- 4.890) nmol/L, respectively. The binding sites of TfR were significantly less in complete remission group than in relapsed or dead groups. There was a positive relationship between the TfR binding sites and the 3H-TdR incorporation in the leukemic cells (AML and ALL) and in the phytohemagglutinin stimulated peripheral mononuclear cells. In both ALL and AML group, the SF, CF were higher and Tf, TIBC were lower than those in control group. TfR binding sites were positively correlated with SF and CF, and negatively with Tf.

Adolescent↗

PubMed1999

Partial left ventriculectomy for the treatment of end-stage dilated cardiomyopathy.

OBJECTIVE: To report our initial experience of the first 5 cases of partial left ventriculectomy (PLV or Batista operation), a new surgical therapy for treatment of end-stage dilated cardiomyopathy in China. METHODS: From April to August 1998, 5 patients with idiopathic dilated cardiomyopathy received PLV at Anzhen Hospital. The mean age of the patients was 48.4 years (range: 42 to 53 years). Three patients were in New York Heart Association (NYHA) class III and 2 in class IV. All patients had mitral valve insufficiency (3 moderate and 2 severe) and 1 had severe tricuspid valve insufficiency in addition preoperatively. Cardiopulmonary bypass and cardiac arrest with cold blood cardioplegia were used in all patients. The average weight of the resected left ventricle muscle was 46.3 g. All patients underwent mitral annuloplasty and 1 plus tricuspid annuloplasty (De Vega). RESULTS: Echocardiography demonstrated a significant decrease in left ventricular diastolic diameter (8.4 +/- 1.1 cm to 5.4 +/- 0.4 cm, P < 0.01) and an increase in ejection fraction (17.78% +/- 6.26% to 34.82% +/- 3.18%, P < 0.025). One patient died of heart failure on the 6th postoperative day. The mean follow-up period of the 4 discharged patients were 4 months (from 3 to 6 months). One were in NYHA class I and 3 in class II; all had a normal life. CONCLUSION: Batista operation is a new and valuable alternative of the surgical treatment for end-stage dilated cardiomyopathy and the long-term results need further studies.

Adult↗

PubMed1999

[Clinical study on effect of Chinese herbal medicine for supplementing kidney and qi and activating blood circulation in treating intrauterine growth retardation of fetus].

OBJECTIVE: To explore the therapeutic effect and its possible mechanism on using Chinese herbal medicine (CHM) for supplementing Kidney and Qi, and activating blood circulation in treating intrauterine growth retardation of fetus (IUGR). METHODS: Fifty-five cases of IUGR were observed, 30 of them were treated with CHM and 25 treated with amino acid for control. RESULTS: The neonatal birth weight in the CHM group was markedly greater than that in the control group. Not only the maternal fundal height and abdominal circumference, but also the fetal growth parameters, including biparietal diameter, head circumference, abdominal circumference, femur length in the CHM group increased much faster than those in the control group. After CHM treatment, the maternal serum levels of estriol (E3) and human placental lactogen (hPL) were close to the normal pregnancy group, but the levels in the control group were significantly different from the normal range. The umbilical venous plasma concentration of essential amino acids was markedly improved after treatment, however, the improvement in the CHM group was more significant than that in the control group. No adverse effect of CHM was found both in mother and fetus. CONCLUSION: CHM for supplementing Kidney and Qi, and activating blood circulation was more effective in the improvement of placental function and amino acid transportation than those of amino acid.

Adult↗

PubMed1999

[Comparative study of ventilatory functions of smokers with MTTp and F-V curve configuration].

To assess the clinical significance of partial mean transit time(MTTp) and F-V curve configuration for testing small airway functions in smokers, routine ventilatory functions, MTTp and F-V curve configuration were studied in 116 passive and active light, heavy smokers. Routine ventilatory functions and F-V curve configuration were determined with routine methods. MTTp were calculated with "convenient calculation". The results showed that the distribution of convex type of F-V curve rose with the degree of smoking. The decrease of V50, V25 and prologation of MTT45%-55%, MTT70%-80% in convex type were just correspondent with heavy smokers. The range of prolongation of MTT45%-55% and MTT70%-80% was 110%-120% of predicted value both in heavy smokers and convex type. These showed there were light disturbances in small airways. Therefore, the convex type, the prolongation of MTT45%-55% and MTT70%-80%, and the decrease of V50 and V25 should all be used as sensitive indices for testing small airway functions. The value of MTTp calculated with "convenient calculation" is accurate and easy-to-do so the method is appropriate for clinical application.

Adult↗

PubMed1999

[Significance of specific time constant and F-V curve configuration in evaluating small airway function for smokers].

To evaluate the clinical significance of the specific time constant (S tau) and F-V curve configuration in testing small airway functions for smokers, routine ventilatory functions, S tau and F-V curve configuration were studied in 116 passive and light, heavy active smokers. Routine ventilatory functions and F-V curve configuration were determined with routine method. S tau were calculated from S tau = 1/V. The results showed that the distribution of convex type rose with the degree of smoking, and the decrease of V50, V25 and elongation of S tau 50, S tau 25 in convex type were just consistent with heavy smokers. The range of elongation of S tau 50 and S tau 25 was 120%-130% of predicted value in both heavy smokers and convex type. These showed there were slight disturbances in functions of small airways. Compared with COPD patients previously measured, their S tau 75, S tau 50, S tau 25 all prolongated to higher degree and meant both large and small airways were affected. Therefore, the convex type, elongation of S tau 50 and S tau 25, and decrease of V50 and V25 were sensitive indices for detecting small airway functions. They can be recommended for clinical application.

Female↗

PubMed1999

[Significance of apoptosis status and apoptosis-associated antigen expression in human colorectal adenocarcinoma sequence].

To investigate the effects of apoptosis on colorectal tumorigenesis and its possible biological significance, the apoptotic frequency in primary cultural cell of 9 normal mucosa, 4 adenomas and 9 adenocarcinomas in time period of 2, 12, 24, 48 hour was measured by flow cytometry. The apoptotic cells index (AI) in situ for 15 colorectal normal mucosa, 7 hyperplastic epithelial, 25 adenomas and 77 adenocarcinomas was identified by the terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end labeling technique (TUNEL). Ki-67 proliferate index (KI), wafl and p53 genes were immunostained with ABC method. The results showed that culture related apoptotic incidence was obviously decreased in cultural tumor cell when compared with mucosa cell after 24-48 hour in vitro. There was a directly positive relationship between the spontaneous apoptosis and Ki-67-index in vivo. The well differentiated or early stage lesions with intensive bcl-2 expression were significantly more likely to have low AI and KI. Both mp53 accumulation and wafl depression which mainly related to KI, had no apparent correlation with AI, bcl-2/bax expression and clinicopathological features statistically; elevatory bax/bcl-2 and pervasive wafl depression led to an increasing AI/KI both in adenoma with atypia and in advanced cancer with distant metastasis or embolus, comparatively. The data indicated that the reduction of susceptibility to inductive apoptosis may contribute to the early phase of tumorigenesis, that AI in vivo may reflect proliferative activity, and that bcl family was closely associated with spontaneous apoptosis and biological behavior of human colorectal cancer.

Adenocarcinoma↗

PubMed1999

[Characteristics of chord time constant and F-V curve configuration in testing small airway functions for smokers].

UNLABELLED: To study the significance of chord time constant (tau ch) and F-V curve configuration in testing small airway function for smokers, the routine ventilatory functions, chord time constant (tau ch) and F-V curve configurations were studied in 116 passive and active (light, heavy) smokers. The tau ch was calculated with Knudson's method, tau ch = V/V. RESULTS: The distribution of convex type rose with the degree of smoking. The tau ch50 in heavy smokers and convex F-V curve prolongated to 130% of predicted value; tau ch50 in light smokers prolongated to 120%. These reflected that there were different degrees of disturbance in small airway functions of smokers and suggested that tau ch50 should be a sensitive and specific index for testing small airway functions. On the other hand, the tau ch75, tau ch50 and tau ch25 by the present authors previously measured in patients with chronic obstructive pulmonary diseases (COPD) all prolongated with the severity of the disease, which reflected that both large and small airways were affected. Therefore it is concluded that in terms of clinical implications the specificity of tau ch50 for small airway functions and the prolongation of tau ch with the severity of COPD are useful indexes.

Adult↗

PubMed1999

[Expression of vascular endothelial growth factor and tumor angiogenesis correlates with biological behaviors of nasopharyngeal carcinoma].

OBJECTIVE: To study the correlation between tumor angiogenesis and biological behaviors of nasopharyngeal carcinoma(NPC). METHOD: Angiogenesis was assessed by the microvessel dinsity(MVD) and expression of vascular endothelial growth factor(VEGF) was detected using immunohistochemical S-P method. Paraffin-embedded specimens from 65 patients with NPC and 15 with benign nasopharyngeal tumors, 15 with tumor-free nasopharyngeal mucosac were detected. RESULT: Expressions of VEGF and MVD in NPC group were higher than that of nonmetastic carcinoma group. The relationship between expressions of VEGF and MVD and the clinical staging present statistic significance. CONCLUSION: MVD and expression of VEGF are highly correlated with biological behaviors of NPC, which may serve as a parameter for determining tumor biological metastatic potential and prognosis. Angiogenesis inhibitors might be helpful in preventing NPC from metastasis.

Adult↗

PubMed1999

[Relationship between expression of estrogen receptor progestrone receptor and the biological characteristics of nasopharyngeal carcinoma].

OBJECTIVE: To Clarity the relationship between the expression of the estrogen receptor (ER), progestogen receptor (PR) and the biological characteristics of nasopharyngeal carcinoma (NPC). METHOD: The immuno-histochemical LSAB method was used to analyse the ER and PR in paraffin section specimen of 71 cases of nasopharyngeal carcinoma. CT scaning and clinic presentation were also put into consideration. RESULT: The expression of ER with (-), (+), (++) and (+++) in 71 cases were: 4(5.63%), 19(26.76%), 27(38.03%) and 21 (26.76%), respectively. The expression of PR with (-), (+) and (++) in 71 cases were: 41(57.75%), 29 (39.44%) and 2(2.82%), respectively. The encroaching of tumor to the tissue of parapharynx, the metastasis rate of lymphonode and clinic stage were related to the expression of ER (P < 0.01). The encroaching of tumor to the tissue of parapharynx, the metastasis rate of lymphonode were also assciated with the expression of PR (P < 0.05). CONCLUSION: NPC is a tumor of hightly expression of ER and PR. The positive intensity of ER and PR were associated with the encroaching extent of NPC, and the metastasis rate of lymphonode. It is significant to detarmine ER and PR of NPC in assessing the biological characterisitics of NPC and predicting the prognosis of NPC. It also suggests that the endocrine therapy can be put into consideration.

Adult↗

PubMed1999

[The observation of mycology and clinical efficacy in 325 cases with otomycosis].

OBJECTIVE: To study otomycosis of pathogenic fungi, antifungal susceptibility test and clinical efficacy. METHOD: The study was performed as follows: 1) culture of pathogenic fungi and fungus identification in 110 cases. 2) antifungal susceptibility test with Etest strip by ketoconazole (KE), itraconazole (IT), Fluconazole (FC), Fluorocytosine (FL), and amphotericinum B (AP). 3) Topical application of doktarin ps cream (DC), ketoconazole cream (KEC), clotrimazole ung (CU), and thymol alcohol (TA) were performed in 4 groups and the intermittent impulsive treatment in who failed to respond to local treatment were performed too. RESULT AND CONCLUSION: 1) Chiefly, otomycosis of pathogenic fungi were aspergillus (79%) and candida albicans (8%) and penicillium citrinum (5%). 2) Antifungal susceptibility test showed that aspergillus were sensitive to KE and IT, but not sensitive to KE and FL, and candida albicans were sensitive to KE, IT, FC, FL and AP all; P. citrinum was sensitive to AP, FL and KE but not to FC. 3) Effective rate of topical application on 4 groups were 97.6%, 97.5%, 90.0% and 80.0% respectively. Comparing the results between compound azoles drugs and TA, there was a statistical significant difference (P < 0.05), the efficacy of compound azoles is better than that of TA. The effective rate of systematic intermittent impulsive treatment in 30 cases failed to respond to local treatment were 100%.

Adolescent↗

PubMed1999

[Study on relative factors in endoscopic sinus surgical procedure].

OBJECTIVE: In order to investigate the factors which affect the operative treatment effect of FESS. METHOD: 215 cases suffered functional endoscopic sinus surgery (FESS) from May 1994 to December 1997 were analysed. RESULT: The clinical curative rate was 75.8% and the improving rate was 96.3%. CONCLUSION: The effect shows that surgical procedure is closely related to following factors: mechanic of operation, drugs taken in pre-operation, kinds of anesthsia, clinical stage of chronic sinusitis, nasal poplys and skill of surgeons.

Adolescent↗

PubMed1999

[Experimental study on pharmacological action of fukean tablet on gastrointestinal tract].

The pharmacological effects of FuKean Tablet(FKT) on gastrointestinal tract were studied. The results showed FKT cured the diarrhea induced by rhubarb or senna, prominently reduced the quantity of feces and delayed the time of excreting charcoal powder in the diarrhea induced by rhubarb or senna model NIH mice; FKT inhibited intestinal propelling-movement in diarrhea induced by senna mmodel NIH mice. FKT inhibited the automatic contraction and Ach induced spasmodic contraction of isolated rabbit intestine(decreased the frequency and intensity of contraction). The above results revealed that the pharmacological base of curing the urgent disease in gastrointestinal tract may be curing the diarrhea, inhibiting intestinal propelling-movement and inhibiting the contraction of intestine.

Animals↗

PubMed1999

[Influence of radiotherapy on reconstruction flaps in oral and maxillofacial regions].

OBJECTIVE: To evaluate the influence of radiotherapy on reconstruction flaps in oral and maxillofacial regions and the radiation tolerance of flaps, and therefore provide clinical information for perioperative radiotherapy. METHODS: Sixty-six free flaps and 22 pedicled flaps were used in the reconstruction of oral and maxillofacial tumor defects. The success and healing of the total 88 reconstruction flaps(14 flaps in preoperative radiation group and 74 flaps in postoperative radiation group) were observed. The acute and late side effects of radiotherapy in the flaps of the postoperative group were studied as well. RESULTS: Of the preoperative 14 flaps, 12 were successful (85.7%), one partially successful and one failed. Of the postoperative 74 flaps, however, 73 were successful (98.6%) and only one partially successful. No total loss of the flap was noted in the postoperative group. The success and healing rates in the preoperative group were lower than those in the postoperative group significantly (P < 0.01-0.05). The rate of acute radiation response of flaps in the postoperative group was significantly lower than that in the surrounding normal tissues(35.1% versus 83.8%, P < 0.01). The late side effects of radiotherapy were rare. The radiation tolerance in different kinds of flaps was similar. CONCLUSION: Reconstruction flaps in oral and maxillofacial regions have a good radiation tolerance and routine postoperative radiation doses can be safely delivered.

Adult↗

PubMed1999

[Study on application of crosslinked chitosan to the speciation analysis of selenium].

The adsorption of Se(IV)/Se(VI) by crosslinked chitosan was studied and the adsorption mechanism was concerned. The results indicated that the adsorption of Se(VI) was 95% at pH=4, while almost no adsorption for Se(IV). The adsorbed Se(VI) was quantitatively desorbed with 1 mol/L HCl after adsorption of Se(VI). A new method for the determination of trace Se(IV)/Se(VI) and organic selenium in environmental samples with hydride generation and atomic absorption spectphotometry has been developed. The detection limit of this method was 20 ng/L, the recovery was 93%-106% and the coefficient of variation was 2.0%-5.0%.

Adsorption↗

PubMed1998

B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion.

Lymphotoxin (LT)alpha is expressed by activated T cells, especially CD4(+) T helper type 1 cells, and by activated B and natural killer cells, but the functions of this molecule in vivo are incompletely defined. We have previously shown that follicular dendritic cell (FDC) clusters and germinal centers (GCs) are absent from the peripheral lymphoid tissues of LTalpha-deficient (LTalpha-/-) mice. LTalpha-/- mice produce high levels of antigen-specific immunoglobulin (Ig)M, but very low levels of IgG after immunization with sheep red blood cells. We show here that LTalpha-expressing B cells are essential for the recovery of primary, secondary, and memory humoral immune responses in LTalpha-/- mice. It is not necessary for T cells to express LTalpha to support these immune functions. Importantly, LTalpha-expressing B cells alone are essential and sufficient for the formation of FDC clusters. Once these clusters are formed by LTalpha-expressing B cells, then LTalpha-deficient T cells can interact with B cells to generate GCs and productive class-switched antibody responses. Thus, B cells themselves provide an essential signal that induces and maintains the lymphoid microenvironment essential for GC formation and class-switched Ig responses.

Animals↗

PubMed1998

Negative regulation of granulocytic differentiation in the myeloid precursor cell line 32Dcl3 by ear-2, a mammalian homolog of Drosophila seven-up, and a chimeric leukemogenic gene, AML1/ETO.

The polyomavirus enhancer binding protein 2alphaB (AML1/PEBP2alphaB/Cbfa2) plays a pivotal role in granulocyte colony-stimulating factor (G-CSF)-mediated differentiation of a myeloid progenitor cell line, 32Dc13. In this article, we report the identification of a PEBP2alphaB interacting protein, Ear-2, an orphan member of the nuclear hormone receptor superfamily that directly binds to and can inhibit the function of PEBP2alphaB. Ear-2 is expressed in proliferating 32Dc13 cells in presence of interleukin 3 but is down-regulated during differentiation induced by G-CSF. Interestingly, AML1/ETO(MTG8), a leukemogenic chimeric protein can block the differentiation of 32Dc13 cells, which is accompanied by the sustained expression of ear-2. Overexpression of Ear-2 can prevent G-CSF-induced differentiation, strongly suggesting that ear-2 is a key negative regulator of granulocytic differentiation. Our results indicate that a dynamic balance existing between PEBP2alphaB and Ear-2 appears to determine the choice between growth or differentiation for myeloid cells.

Animals↗