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Biomedical subjects

G I Abelev

Publications and source records attributed to G I Abelev.

13 recordsLinked to original sources
PubMed1979

[Counter-flow isotachophoresis on cellulose acetate membranes. Role of electroendosmosis].

A variant of counter-flow isotachophoresis of proteins on cellulose acetate membranes is proposed. The liquid counter-flow is created by electroendosmosis in the membrane. Proteins are concentrated at the Kolrausch boundary during isotachophoresis in the presence of ampholytes. The method permits one to make microanalysis of proteinic mixtures in diluted solutions, and it can be used in combination with immunodiffusion and immunoelectrophoretic methods of antigenic protein detection.

Antigens↗
PubMed1979

[Two-layer rocket-electrophoresis on cellulose-acetate membranes].

Two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "Cellogel" has been developed. The method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. The procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitates with antiglobulin antibodies, the complement or their combination. An increase in the method sensitivity up to 30-60 ng/ml in the visible zone of the reaction is achieved by simultaneous reduction in the antibody concentration and the growth of the absolute quantity of the antigen, subjected to electrophoresis, up to 50-100 microliters. The method has been applied to human alpha-fetoprotein.

Electrophoresis, Cellulose Acetate↗
PubMed1978

[Immunoisotachophoresis on cellulose acetate film].

Acetate-cellulose strips of "Cellogel" type have been shown to be a suitable maintenance medium for performance of isotachophoresis. For immuno-isotachophoresis antigen (from 0.5 to 20 microliter) is applied to a strip of acetate-cellulose film. 1--2 microliter of ampholine solution is placed in front of the antigen zone. All the components present on the strip are made in 0.06 M tris-HCl buffer (pH 6.7), and 0.012 M tris-glycine (pH 8.3) is used as an electrode buffer. Electrophoresis produces migrating Kolraush boundary, which at first is the area of antigen concentration into a narrow starting zone, and then of antigens separation with ampholites. The antigens separated on a cellogel strip are subject to cross-electrophoresis on a film saturated with the respective antiserum, with formation of precipitation peaks for each individual antigen. The method permits to operate with low antigen concentrations since electrophoresis ensures their preliminary concentration and the width of the zones is independent of the time of separation.

Animals↗
PubMed1977

[In vitro synthesis of alpha-fetoprotein by individual hepatocytes and microcolonies of human hepatocytes].

Alpha-fetoprotein (AFP) produced by individual hepatocytes and hepatocyte microcolonies was detected with microelectrophoresis-precipitation in polyacrylamide gel. Hepatic cells of 6--13-week-old human embryos were cultivated in vitro for 2 to 5 days. 23 of 28 individual cells, and 89 of 91 microcolonies, built up of 2--35 cells, were demonstrated to produce AFP within the range of 70--800 pg per cell.

Cells, Cultured↗
PubMed1977

[Synthesis of alpha-fetoprotein and albumin human embryo hepatocytes].

Population aspects of specific secreted proteins (alpha-fetoprotein and serum albumin) were analyzed in the cultured human embryo hepatocytes (6 to 12 weeks' gestation). A method based on local hemolysis in gel of sheep erythrocytes conjugated with antibodies specific of proteins in question. The great majority of individual hepatocytes synthesized both proteins.

Cells, Cultured↗
PubMed1977

[Highly sensitive electroimmunodiffusion method of detecting antigens on cellulose acetate film].

A highly sensitive electro-immunodiffusion test suggested by the authors for antigen detection on cellulose-acetate films consists of three stages: antigen concentration in a discontinuous buffer system on cellulose-acetate films; antigen detection on the same films by immunodiffusion using standard test system; to detect the precipitation bands the washed films are stained with protein dyes in case the reaction takes place in the zone of vision, or subject to further treatment by means of "plating" the precipitates with antiglobulin antibody or by radioautography. The method permits one to reveal the nanogram levels of alfa-fetoprotein and it may be applied for detection of antigens with different molecular weights and electrophoretic mobility.

Acetates↗
PubMed1976

Specific antigen of murine erythroblasts.

A new antigan has been revealed by means of antisera against Rauscher virus in mice with Rauscher virus-induced leukemia. This antigen appears to be different from both Rauscher type-specific antigen and MULV-gs-1 (p-30), as shown by studies of electrophoretic mobility and immunochemical specificity. Except in leukemic mice it was also found in low levels in both serum and spleen extracts of healthy mice of a number of strains. Furthermore, this antigen was regularly demonstrated by immunofluorescence on the surface of erythroblasts, but not on the surface of erythrocytes, lymphocytes, polymorphonuclear cells and thymocytes, and was shown to be different from fetal hemoglobin. Therefore, it is referred to as antigen of erythroblasts (Ag-Eb), which seems to represent a surface marker for a certain differentiation stage of erythroid cells.

Animals↗
PubMed1976

[Detection of the base level of alpha-fetoprotein in the sera of donors by the electrophoresis-precipitation of polyacrylamide gel method].

Alpha-fetoprotein (AFP) was detected in the sera of adult healthy persons by the method of electrophoresis-precipitation in polyacrylamide gel; it was distinctly determined in about 50% of cases, and its concentration was not over 3ng/ml. AFP was determined all the 20 cases suffering from cirrhosis of the liver examined by the mentioned method.

Electrophoresis, Polyacrylamide Gel↗
PubMed1975

[Ultramicromethod of determining antigens].

A method of alpha-fetoprotein (AFP) ultramicrodetection, permitting to reveal up to 10(--8) mg of AFP with its concentration of 1--2.10(--6) mg/ml was suggested. The antigen ic concentrated in a capillary tube during the electrophoresis in polyacrylamide gel. The compressed zone moves into the gel plate, containing antiserum. Free diffusion of the antigen is followed by the formation of precipitation rings, whose area is in proportion to the amount of the antigen. The precipitins are revealed by the treatment with I125-labeled antibodies to gamma-globulin of the immune serum, with subsequent radioautography.

Animals↗