Human decidual CD7+ lymphocytes display a unique antigenic phenotype.
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Antigens, CD↗
Biomedical subjects
G I Bondarenko
Publications and source records attributed to G I Bondarenko.
Phenotypic characterization of CD7+, CD3+, and CD8+ lymphocytes from first trimester human decidua using two-color flow cytometry.
PROBLEM: There is increasing evidence that decidual lymphocytes play a major role in local interactions at the fetomaternal interface. METHOD: In this paper we use two-color flow cytometry to delineate the phenotype of lymphocytes obtained from human early decidua by mechanical dispersal technique. RESULTS: The most abundant decidual lymphocytes expressed CD7, CD38, CD56, and CD2 markers, relatively small proportions of CD3+, CD8+, CD4+, CD16+, CD45RA+, CD11b+, and Leu8+ cells were also present. The vast majority of decidual CD7+ lymphocytes expressed CD38, CD2, and CD56 markers and were CD3-, CD8-, CD16-, and CD57-. Decidual CD3+ lymphocytes were weakly staining for TCR alpha/beta, lacked T-cell receptor (TCR) gamma/delta molecules, and approximately 40% of them expressed HLA-DR. All decidual CD8+ lymphocytes were CD2+ and the majority of them expressed CD38, CD56, and CD7 markers and were CD3- at the same time CD8+CD7- lymphocytes were found in decidua. According to the expression of the CD45RA marker, decidual CD8+ lymphocytes could be divided into two subsets: CD8+CD45RA+CD56+ and CD8+CD45RA-. CONCLUSIONS: These data clearly demonstrate that decidual lymphocytes display phenotypical features different from those of their counterparts in peripheral blood.
Antigens, CD↗
[Reduction of nitro-substituted compounds by native and immobilized Escherichia coli cells].
Reduction of nitro-substituted compounds, 1,4-benzodiazepine-2-ones, dibenzo[b,f]-1,4-diazepines, quinolones, and quinoxalinones, by Escherichia coli cells was studied. Physicochemical methods demonstrated the formation of corresponding amines. 4-(p-Nitrophenyl)-1H-6-R-quinolones-2 were nor reduced by Escherichia coli cells. Regiospecific reduction of 2,4-dinitro-5H-11-(p-R-phenyl)-dibenzo[b,f]-1,4-diazepines and 4-(2'-R-3',5'-dinitro)-benzoyl-3,4-dihydroquinoxalinones-2 was shown to result in the formation of 2-nitro-4-amino-5H-11-(p-R-phenyl)-dibenzo[b,f]-1,4-diazepines and 4-(2'-R-3'-nitro-5'-amino)-benzoyl-3,4-dihydroquinoxalinones-2, respectively. Methods for microbiological reduction of nitro compounds and immobilization of Escherichia coli cells into carrageenan and its modified forms were elaborated.
Cells, Immobilized↗
[The selective reduction of nitro compounds by E. coli cells].
Nitroreduction of 2,4-dinitro-5H-11-p-R-phenyl-[b,f]-1,4-diazepines and 4-(2'-R-3',5'-dinitro)benzoyl-3,4-dihydroquinoxalinones-2 by E. coli with formation of 2-nitro-4-amino-11-p-R-phenyldibenzo-[b,f]-1,4-diazepines and 4-(2'-R-3'-nitro-5'-amino)-benzoyl-3,4-dihydroquinoxalinones-2 has been demonstrated using a set of physical and chemical methods.
Biodegradation, Environmental↗
[Reduction of nitro-substituted 1,2-dihydro-3H-1,4-benzodiazepine- 2-ones by E. coli cells immobilized in carrageenan].
Reduction of nitro-substituted 1,2-dihydro-3H-1,3-benzodiazepine-2-ones by E. coli cells immobilized in carrageenan was studied. The corresponding amines are the sole products with a 100% yield as compared to the native cells. Conditions for immobilization of E. coli cells in the home-produced carrageenan was worked out: the cell to carrageenan ratio is 1:10 (w/w), granulation in toluene at 0-(+)4 degrees, treatment with 0.3-0.4 M KCl. The carrageenan-immobilized cells are stable upon storage, repeated usage (after 10 cycles about 80% of the initial activity is retained), and when being used in column fermenters.
Benzodiazepinones↗
[Volume increase coefficients for solutions and suspensions of several pharmaceutical substances].
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Drug Compounding↗
[Mononuclear cells and specific proteins in the tissues of the fetoplacental complex during the physiological course of pregnancy and miscarriage].
Subsets of mononuclear cells, specific pregnancy proteins (SP-1, SP-3, PAPP-A) in the normal pregnancy decidua (NPD) and spontaneous abortion decidua (SAD), and trophoblast were studied. Significant decrease of lymphocytes infiltration was found in SAD. CD8+ cells rather than CD4+ cells predominated in NPD. CD4+ cells levels were lower in NPD in comparison with SAD. CD4+ lymphocytes rather than CD8+ cells predominated in SAD. Macrophages population was found to be increased in SAD in comparison with NPD. In blood vessels walls, cytoplasm of mononuclear cells and decidua stroma, PAPP-A and SP-3 were expressed in NPD and unexpressed or negative in SAD. In syncytiotrophoblast, SP-1 was expressed in normal pregnancy and unexpressed in spontaneous abortion. It is suggested that inadequate fetal-placental tissues counts of suppressor decidual lymphocytes, specific pregnancy proteins might be a cause of early spontaneous abortion.
Abortion, Spontaneous↗