Antibody formation to Babesia bovis and Anaplasma marginale in calves in Bolivia.
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Biomedical subjects
Publications and source records attributed to G Ibata.
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A monoclonal antibody based competition-ELISA is described for the detection of pestivirus antibodies directed against conserved epitopes on the p80 viral protein. The ELISA detected increases in serum antibody following experimentally induced infections of pigs, cattle and sheep with a wide range of pestiviruses, although the sensitivity of the test was not uniform for the different viruses studied. The ELISA was compared with virus neutralization tests for the assessment of porcine, bovine and ovine field sera. At a cut-off value of 50% inhibition, the ELISA showed a high specificity relative to virus neutralization tests, but appeared less sensitive for the detection of some weakly positive samples from pigs. Sera from both ruminants and pigs could be assessed without any modification of the test.
A pestivirus originally isolated from weaner pigs was shown to be capable of infecting weaners experimentally, but without inducing significant signs of disease. When inoculated into pregnant sows and ewes in early gestation, both the porcine virus and an antigenically similar ovine border disease isolate could induce congenital infections in both species.