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Biomedical subjects

G Ittrich

Publications and source records attributed to G Ittrich.

18 recordsLinked to original sources
PubMed1982

[Determination of HCG excretion for diagnosis of trophoblast vitality and assessment of age of early pregnancy (author's transl)].

A convenient technique for quantitative determination of human chorionic gonadotrophin in urine is described in this paper, Gravimun test kit reagents are used to establish inhibition of haemagglutination, using in glass tubes one tenth (Gravimun) or one fifteenth (Gravimun II) of the amounts of reagents which would be required for the original test. Erythrocytes undergo sedimentation as two longitudinal stripes in parallel on the bottom of the tubes. The spacing between them is taken as the measure of the HCG quantity added to the system and related to a specially prepared standard curve. The Second International Standard of HCG for Bioassay is used for comparison. - Relation c = t3 (c = HCG concentration, IU/L; t = days from nidation, 20 days from last menstruation) was established for this period of time from values which had been recorded from 500 normal early pregnancies up to the tenth week. That relation can be used as a rule of thumb by which to determine age of pregnancy or to calculate expected average HCG excretion up to the tenth week of pregnancy.

Chorionic Gonadotropin↗
PubMed1980

[On the reactivation of atrophic oral mucosa through topical application of 17 beta-estradiol (author's transl)].

Dental plastics are capable of absorbing therapeutically effective amounts of estrogens and giving them off continuously for a period of several months. Kallocryl C enriched with 17 beta-estradiol has the effect of triggering keratinization of atrophic oral mucosa of the dentural support. There is no fear of this form of application leading to hyperkeratosis. Subjective complaints such as, for example, oral mucosal burning are either alleviated or eliminated. Hormonal side effects have not been observed.

Aged↗
PubMed1980

A simple technique for quantitative measurement of erythrocyte settling patterns in hemagglutination inhibition tests for immunologic hormone assay.

A simple technique for direct quantitative evaluation of hemagglutination or hemagglutination inhibition tests used in immunologic proteo-hormone assay systems is described. The test is performed in calibrated glass capillaries (internal diameter 2.5...4 mm) at horizontal position. After sedimentation the non-agglutinated erythrocytes form two fine parallel stripes along the bottom of the capillaries. The distance between the stripes depends on the amount of additional free antigen brought into the system. It can be measured by the slit of a micrometer screw or a corresponding equipped microscope and might be used as y-variable for drawing calibration curves. At well standardized test conditions and for selected concentration ranges coefficients of variation between 10 and 15% can be achieved. So quantitative determinations can be made without serial sample dilutions to be prepared, covering concentrations ranges between 1 to 10.

Chorionic Gonadotropin↗
PubMed1979

[Release of ovulation by application of gonadotrophin as well as by radio-immunological assay of oestradiol and progesterone in serum (author's transl)].

The use of gonadotrophins to release ovulation is indicated for women with urgent desire for children and no positive response to clomiphene therapy. (Hence, a negative selection is made.) --The authors checked 100 therapeutic gonadotrophin series, with 69 patients involved. Oestrogens and progesterones were followed up by radio-immunological hormone analysis. --Ovulation occurred in 54 cases. Twelve patients developed ovarian reaction with oestrogen rise, while no ovarian response at all was recordable from 34 patients. Six women became pregnant under gonadotrophin therapy and two following the treatment. The results are compared with literature data.

Body Temperature↗
PubMed1978

A simple and rapid technic for radioimmunoassay and calculation of plasma steroid concentrations.

A simple RIA technic for plasma steroids is described: Extraction residues or reference standards are dissolved in 20 microliter ethyl alcohol, or 20 microliter of alcoholic plasma extracts are given directly into RIA tubes; 500 microliter tracer solution, containing a known tracer mass, and, after mixing, 500 microliter antiserum solution or buffer solution are added, followed by 20 min incubation at room temperature and 20 min standing in an ice bath. Free from bound activity is separated by charcoal coated florisil or silicagel. Plasma steroid levels are calculated by a simple equation. An immunologic equivalent to the tracer mass present in the reaction mixture serves as reference value. Drawing of standard curves is not necessary for every assay. The linear transformation used here allows the number of reference standards to be reduced. So also single samples can be analyzed with relatively low effort.

Estradiol↗
PubMed1977

[Studies on the excretion of estrogen in cows following oral and percutaneous (pour-on) administration of chlormadinone acetate].

The oestrogen levels were assessed in 180 urine samples taken from 20 cows. The animals had received over 15 days 40 mg one-per-cent oily solution of chloromadinone acetate. The solution had been applied orally or by pour-on methods. The curves of oestrogen excretion were identical for both methods of application, both during and after completed administration. In other words, the effect obtainable from pour-on application is just as good as that obtainable from oral administration.

Administration, Oral↗
PubMed1977

[A simple method for the radioimmunologic determination of sex steroids in plasma (author's transl)].

A simple method for the determination of plasma steroids by radioimmunoassay is described. Essential steps are: Dissolution of extraction residues in 20 microliter ethyl alcohol or direct addition of 20 microliter alcoholic plasma extract to the incubation tubes, addition of tracer solution with known tracer mass and antiserum dilution or gelatine phosphate buffer, incubation for 40 min and separation of free from bound radioactivity by charcoal-coated florisil. Plasma steroid levels are calculated by a simple equation, an immunologic equivalent to the tracer mass serves as the reference value.

Estradiol↗