Biomedical subjects
G J Marell
Publications and source records attributed to G J Marell.
Quantitative determination of non-haem iron and ferritin iron in bone marrow using flameless atomic absorption spectrophotometry. A comparative study on the cytological and chemical determination of the bone marrow iron content.
A method for non-haem iron analysis in bone marrow aspirates using graphite furnace atomic absorption spectrophotometry has been developed. Bone marrow aspirates were obtained from patients with various disorders. A good correlation is observed between chemical and cytological assessment of total non-haem iron in bone marrow. An intra-assay coefficient of variation of 9.0% was observed. The ferritin-iron concentration was also determined and a CVduplo of 11% was found. The ferritin iron concentration increased with an increasing total iron content until saturation of ferritin appeared to be reached at about 3 g ferritin per kg protein. It was concluded that the quantitative determination of bone marrow iron can be of value in the diagnosis and investigation of both hypo- and hyper-ferraemic disorders.
Rapid and sensitive immunochemical determination of immunoglobulins by centrifugal analysis.
The measurement of immunoglobulin concentrations in serum by a turbidimetric method using a centrifugal analyser (Cobas Bio) is described. In the procedure described it is possible to measure the absorbance of each sample in the buffer reagent in a preliminary run without antiserum (sample blank for correction of colour, turbidity, etc.) In the main run (after adding antiserum) a final measurement is made after 600 seconds reaction time and the difference between the final absorbance and the sample blank is corrected for the absorbance of the antiserum determined in the antiserum blank cuvet. The initial phase of the reaction can thus be taken into account so that high titre antisera can be used. Therefore the method covers a wide range of values with good precision.
Protein determination in the assay of hormone receptors.
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