Biomedical subjects

G J Mohr

Publications and source records attributed to G J Mohr.

3 recordsLinked to original sources

PubMed2001

Fluoro reactants and dual luminophore referencing: a technique to optically measure amines.

An optical sensor for aqueous 1-butylamine is presented which combines two novel techniques: A fluorescent indicator dye (fluoro reactand) embedded in a thin polymer layer performs a reversible chemical reaction with the analyte, causing changes in luminescence intensity. At the same time, inert phosphorescent beads dispersed within the polymer layer provide luminescence signals that act as an internal reference for the indicator dye. As a consequence, the optical sensor is independent of light source fluctuations, ambient light, drifts in optoelectronic setup, or optical fiber bending.

Journal Article↗

PubMed2001

Tailor-made dyes for fluorescence correlation spectroscopy (FCS).

Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.

Benzopyrans↗

PubMed1995

Enzyme biosensor for urea based on a novel pH bulk optode membrane.

A new, absorbance-based enzymatic biosensor membrane for determination of urea is described. A lipophilic, fully LED- and diode laser-compatible pH sensitive dye was incorporated into a plasticized, carboxylated poly(vinyl chloride) membrane and served as the optical transducer of the sensor. Urease was covalently linked to the surface of the pH bulk optode membrane to form a very thin cover. The resulting biosensor membrane allows rapid determination of urea over the 0.3 to 100 mM range. The reproducibility, stability, and effects of pH and buffer concentration on the response of sensor are reported. The preparation of the pH transducer and the immobilization of the enzyme are simple and may easily be adopted to other biosensor types.

Animals↗