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Biomedical subjects

G Jäger

Publications and source records attributed to G Jäger.

At least 73 records · Page 4Linked to original sources
PubMed1989

[Vascular purpura and other cardiovascular alterations in children with acute parvovirus B19 infection. Report of 3 personal cases].

Most frequent skin manifestation of parvovirus B19 infection is the erythema infectiosum (fifth disease; Sticker's disease). Vascular purpura (some cases of Henoch-Schönlein purpura) has been reported in virus infections such as infectious mononucleosis (Epstein-Barr virus), hepatitis B, cytomegalovirus etc., and human parvovirus B19 could be another cause. Clinical course, procedure of diagnostics and therapy in 3 own serologically established cases of vascular purpura or severe alterations of the blood circulation due to parvovirus B19 infection are presented. Parvovirus B19 (or other viruses) as a trigger for vascular purpura and alteration of the blood vessel wall and blood circulation could explain the childhood predominance and seasonal clustering of that disease. Furthermore an individual disposion (in form of special HLA type?) for the development of vascular purpura can be suggested.

Cardiovascular Diseases↗
PubMed1987

Myelo-monocytic differentiation potential of the CALLA-positive Nalm 6M1 cell line.

Nalm 6M1 cells, derived from an acute lymphoblastic leukemia of the non-T, non-B type, are known to possess characteristics of early B-lymphocytes. In order to determine whether these cells are able to differentiate along the granulocyte-monocyte pathway, they were cultured in diffusion chambers, and classified according to morphological, cytochemical and immunological criteria. On the basis of this characterization, Nalm 6M1 cells positively labeling with monoclonal anti-human granulocyte or monocyte-specific antibodies were observed in all cases. These immunological changes were accompanied by the appearance of cells positive for the naphthyl-AS-D-chloroacetate esterase reaction. However, no changes in the blast-like appearance of the cultured cells were seen. From these data, we conclude that Nalm 6M1 cells already committed to the B-cell lineage retain their capacity to develop cellular markers of the granulocyte-monocyte series.

Acute Disease↗
PubMed1983

Tritium autoradiography of cell surfaces in smear preparations.

Experiments were performed in order to find out whether tritium-labeled cell surface markers can be quantified at the single cell level in autoradiographs of smear preparations. Mouse thymocytes were incubated with 3H-concanavalin A and subsequently spread on microscopic slides. The spreading techniques, either by cytocentrifugation or by the use of cover slips, were performed in such a way as to achieve preparations in which the mean flatness of the cells varied. By means of incident light microphotometry, the cellular areas and the grain counts of individual cells were determined. The results show a strong dependence of the mean grain yield per slide on the mean cellular area. Cytocentrifuge preparations resulted in larger mean cellular areas and higher mean grain counts than cover slip preparations. With the use of cytocentrifugation, however, the differences in the flatness of the cells were of such a magnitude that a reproducible quantification of 3H-labeled cell surface markers was not possible. Conventional techniques of spreading cells on slides failed to provide a degree of flatness that could approach the saturation grain count per cell without completely destroying the cellular morphology.

Animals↗
PubMed1982

Auer-rod positive myelocytic leukemia cells in diffusion chambers: differentiation along the eosinophilic pathway.

Auer-rod positive acute myelocytic leukemia (AML) blasts from a 33-year-old patient were cultured in diffusion chambers (DC) in order to test their differentiation potential. The cells were labeled with anti-human granulocyte anti-serum known to be negative for eosinophils, and evaluated using the unlabeled peroxidase-antiperoxidase (PAP) method. Parallel to a decline in the number of leukemic blasts there was an increase of up to 86% in the number of granulocytic cells belonging to the eosinophilic series. Auer-rod bodies were found in the eosinophilic cells even after 20 days in culture. Staining with anti-granulocyte antiserum failed to demonstrate positive cells at any time during DC culture. Based on the negative reaction with the anti-granulocytic antibodies already at an early stage of development evidence is provided for the existence of a progenitor cell exclusively committed to the eosinophilic pathway.

Adult↗
PubMed1982

Induction of cALL antigen and terminal deoxynucleotidyltransferase in normal peripheral lymphocytes during PHA stimulation.

In PHA cultures of peripheral lymphocytes from three healthy donors the extent and time sequence of cALL and TdT positivity was followed over a period of seven days. In all cases significant percentages of cALL+ and TdT+ cells were observed. However, the period of positivity for both these markers tended to be short. In contrast to previous results with the diffusion chamber system which yielded a comparatively uniform pattern of positivity, PHA cultures of the present cases revealed a more heterogeneous pattern concerning the extent as well as the time sequence of positivity. These experiments provide a broader basis for the notion that those markers are expressed during blastogenesis of normal lymphocytes which are generally regarded as indicative of early lymphatic differentiation and of the stage of developmental arrest of acute lymphatic leukemias.

Antigens, Neoplasm↗
PubMed1982

Acute lymphoblastic leukaemia cells in the diffusion chamber: change of surface phenotype during the culture period.

Peripheral blast cells from patients with acute lymphoblastic leukaemia were cultured in the diffusion chamber system. On different harvesting days the cALL antigen, surface immunoglobulin and T-cell antigen were investigated by direct immunofluorescence. Each cell type investigated showed a different response throughout culture, the commonest change observed being development into the B-cell axis. Our data demonstrate that the differentiation stop of a leukaemic cell can be overcome by using the diffusion chamber system. The data therefore give some insight into the stage of developmental arrest in acute lymphoblastic leukaemia.

Antigens, Neoplasm↗
PubMed1981

A simple test for the terminal deoxynucleotidyl transferase using the peroxidase-antiperoxidase technique.

A technique has been worked out for an easy detection of terminal transferase in individual cells by means of the unlabeled antibody enzyme (PAP) method. Terminal transferase is an important enzyme marker for the classification of acute lymphoblastic leukemias that show a variable extent of differentiation into the T-cell series. The incubation procedure giving the best results is described.

DNA Nucleotidylexotransferase↗
PubMed1981

Appearance of B- or T-lymphocyte markers after diffusion chamber culture of acute lymphoblastic leukemia cells.

Blast cells derived from blood and marrow samples of a patient with acute lymphoblastic leukemia (ALL), as well as from the Reh line originally established from an ALL patient, were cultured in diffusion chambers implanted i.p. into preirradiated CBA mice. At different intervals over a period of up to 20 days, surface immunoglobulins, ALL antigen, and T-cell antigen were investigated by using direct immunofluorescence. Rosette formation was tested with sheep and mouse erythrocytes. On day 0, the cells expressed only ALL antigen at the surface, and no rosette formation was observed. During culture the patient's lymphoblasts, which originally had cytoplasmic IgM in addition to ALL antigen, expressed surface immunoglobulins as well as mouse erythrocyte receptors. The Reh line cells were ambivalent in two experiments developing T-cell antigens and sheep erythrocyte receptors as well as mouse erythrocyte receptors. Our data suggest that the differentiation arrest in leukemic lymphoblasts can be overcome, thus entailing a surface pattern similar to mature T- or B-lymphocytes.

Antigens↗
PubMed1981

Discrimination in immunofluorescence between labeled and unlabeled cells by quantitative microfluorometry and computer analysis.

An automated procedure for discrimination in immunofluorescence between antibody-labeled and unlabeled cells has been developed on the basis of microfluorimetric determination of intensity distributions. After smoothing the raw data for irregularities caused by the scoring statistics optimum fit of the negative distribution to the corresponding positive one was achieved. The procedure was tested in a model system by mixing various known proportions of immunofluorescence-negative and -positive plastic beads. In addition, variable mixtures of T-negative CLL cells and normal mononuclear peripheral blood cells were labeled with FITC-conjugated anti-T-antiserum. The expected percentage of T-positive peripheral blood cells agreed satisfactorily with the data measured and computed. Finally, the measured percentage of Ig-positive mononuclear cells from normal peripheral blood was in agreement with the values obtained by other techniques.

Automation↗
PubMed1981

Quantitation of the Pap reaction on single cells by absorption cytophotometry.

The reaction product of the unlabeled antibody enzyme method was quantitated at the single cell level by absorption microphotometry. Attempts were made to demonstrate that the amount of dye deposited on individual cells can be taken as a measure of the number of antibody molecules bound. In an experiment with prefixed sheep erythrocytes loaded in excess with amboceptor a close relationship was observed between cell size and the amount of dye measured. Furthermore, the known differences in A1 antigen density on human A1 and A2 erythrocytes were satisfactorily reproduced with this quantitative approach. As regards T antigen density on human lymphocytes of different origin, the results presented tally with data in the literature. It is therefore concluded that the method is suitable for quantitating antibody amounts bound to individual cells.

Absorption↗
PubMed1981

Expression of common acute lymphoblastic leukaemia antigen and terminal deoxynucleotidyl transferase in normal mononuclear blood cells during diffusion chamber culture.

Peripheral mononuclear blood cells of 2 healthy individuals were cultured over a 13 d period in diffusion chambers. A high percentage of cALL antigen positive cells and later on TdT containing cells appeared during culture. The cALL+ cells could be clearly distinguished into 2 groups: a population with weak and diffuse fluorescence and a small number of ring-like brightly dyed cells. The first group develops into TdT+ cells and therefore resembles immature T-cells. The interpretation of the ring-like stained cells is still preliminary, but it cannot be excluded, that these cells represent a cell population with more than lymphatic differentiation capacity.

Antigens, Neoplasm↗
PubMed1980

[Comments on stillbirth frequency in Suhl region, between 1970 and 1977 (author's transl)].

An analysis is presented in this paper of 525 stillbirths, related to 54,342 births, in the region of Suhl, between 1970 and 1977. The above number of stillbirths was subdivided by main groups of diagnosis and analysed by various parameters. Stillbirth rates were found to be somewhat increased with mothers aged 30 years and above, with the third child and more of one and the same mother, as well as with birth weights below 2,500 g. Stillbirths classified "avoidable" by competent district and regional commissions are critically appraised for their underlying causes. The following conclusions were drawn from the findings and established as demands: Intensification of general and specialised attention to pregnant women, early detection of retardation in embryonic development, high-accuracy control of labour and early detection of any risk to the "intra-uterine patient", early and properly indicated use of intrapartum surgery, and improvement in density of the network of obstetric control methods to include also smaller maternal centers as of 800 childbirths per annum.

Female↗
PubMed1979

Growth of the Reh cell line in diffusion chambers. Evidence for differentiation along the T- and B-cell pathway.

Cells of the Reh line, originally derived from an ALL of the 'Non-T, Non-B' type were cultured in diffusion chambers implanted intraperitoneally into perirradiated CBA mice. At different intervals over a period of 20 d changes in surface characteristics were examined by labelling the cells with AcALLG, ATCG as well as with polyvalent AIg. The evaluation was performed by using direct immunofluorescence. In addition, the ability to form rosetts with SRBC, AET-treated sheep erythrocytes, mouse red blood cells and EoxAC was tested. On day 0 of the diffusion chamber culture the cells only carried cALLA, and no rosette formation was observed. In the course of the diffusion chamber culture the cells unequivocally developed T-cell antigen, and in 1 of 2 experiments they further acquired a receptor for forming AET- and E-rosettes. Conversely, in the other experiment a receptor for mouse red blood cells was detected in a considerable portion of the cells. Our data show that the rather undifferentiated Reh line cells in vitro are able to develop features of mature T-cells and attributes of early B-cells during the diffusion chamber culture. In vitro they apparently retain a bivalent potentiality of lymphatic maturation. The diffusion chamber system proves to be a suitable tool for promoting differentiation in these cells.

Animals↗