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Biomedical subjects

G Jagiello

Publications and source records attributed to G Jagiello.

36 records · Page 2Linked to original sources
PubMed1977

Preliminary evidence for the intracellular localization of luteinizing hormones in mammalian oocytes.

Preliminary autoradiographic and immunofluorescent evidence is presented which suggests the localization of hLH beta, hCG and oLH within the oocytes of several mammalian species. Specificity of the response is supported by negative observations in the presence of cold hormone or antibodies to other proteins. The mechanism of action of these gonadotrophins in such a site remains to be defined.

Animals↗
PubMed1977

Further studies of inhibitors of in vitro mammalian oocyte maturation.

Studies of luteinizing hormone (LH)-mediated serum inhibition and of follicular fluid and granulosa cell inhibition of in vitro porcine, ewe, and cow oocyte meiotic maturation have been carried out. A porcine serum inhibitor or inhibitors with a molecular weight above 15,000, similar to the ewe and cow serum inhibitor(s), have been demonstrated. An LH-inactivated inhibitor or inhibitors in fluid from medium-sized ewe and cow follicles have been shown and a minimally effective porcine follicular fluid inhibitor has been confirmed. The estimated molecular weight of these inhibitors is below 1,000. Granulosa cells from the three species were not found to be dramatically inhibitory. It is postulated that the follicular fluid inhibitor(s) may be derived from the larger serum molecule(s) by an action of LH within an intermediate cell type.

Animals↗
PubMed1976

Cytological observations of deletions in pachytene stages of oogenesis and spermatogenesis in the mouse.

Cytological examination of pachytene stages of oogenesis and spermatogenesis revealed the presence of diagnositc loops in cells heterozygous for each one of six radiation induced lethal albino alleles in the mouse. Such loops were never found in any of the normal sibs or other controls. Such evidence supports the contention that all six of these mutations are deletions as indicated previously for five of them by complementation tests. An incidental finding was that of a pleiomorphic region on a medium sized pachytene bivalent in both sexes. This was not restricted to mutant cells but was also found in control cells. Whereas the pachytene deletion loops characterize lethal albino allele carriers, the observation of the pleiomorphic region reveals an apparently normal cytological phenomenon in the mouse, the significance of which remains to be determined.

Animals↗
PubMed1975

Stimulation and inhibition with LH and other hormones of female mammalian meiosis in vitro.

Utilizing some known physiological preovulatory hormone levels and specific cytological end-points, experiments were carried out with two different media to obtain a clearer understanding of meiotic maturation in vitro of ewe, cow, monkey and human oocytes. In the first series of experiments, it was shown that, in McCoy's medium, ovine LH, prostaglandin E1 and ovine prolactin increased the percentage of divisions of ewe oocytes, without cAMP as a demonstrable intermediate. Monkey oocytes incubated in McCoy's medium with fetal calf serum were stimulated by ovine LH, prostaglandin E2 or cAMP. Human oocytes were stimulated to divide in Diploid medium by ovine LH, oestradiol-17 beta with LH added later, or prostaglandin E1. Diploid medium containing added sheep serum markedly inhibited the meiotic activity of ewe oocytes when ovine LH, HCG, cAMP, prostaglandin E1 and E2 of prolactin were added. This effect was studied by separating the contained fetal calf and sheep sera. It was noted that when LH, HCG, or prostaglandins E1 and E2 were added to a system utilizing fetal calf serum, only spontaneous resumption of meiosis occurred: stimulated and spontaneous activity were reduced when cAMP or prolactin were added. The inhibition of ewe oocyte maturation in Diploid medium with sheep serum and additives was also seen with cow oocytes incubated in Diploid medium containing fetal calf serum and LH, HCG, cAMP or prostaglandin E1 and E2. Cow oocytes incubated in McCoy's medium with equivalent fetal calf serum and added LH were sharply inhibited but spontaneous maturation persisted in the presence of added HCG, cAMP or bovine prolactin.

Animals↗
PubMed1975

Aging and X chromosome loss in the human ovary.

A study of the incidence of X chromosome loss from the mitotic complement of human ovary has demonstrated a small loss beginning between ages 36 and 45 years and increasing additively to age 75. This was earlier and less than has been shown for blood. In ovarian tissue, autosomal chromosomal loss declined with age while X loss increased. Though no correlation of X loss could be demonstrated with the clinical state of the menstrual cycle, there was a greater loss of all chromosomes in normally cycling subjects than in perimenopausal and postmenopausal subjects. The possibility that the menopause is initiated in the ovary is raised by findings of these correlates of aging.

Adult↗
PubMed1967

Streptonigrin: effect on the first meiotic metaphase of the mouse egg.

Streptonigrin, an antibiotic and antitumor agent, alters the chromosomes of the mouse ovum during meiosis. Agglutination of bivalents or achromatic gaps and breaks occurred in the larger pairs both in vitro and in vivo. This newly detected cytogenetic effect suggests that such agents can gain access to developing mammalian ova and destroy the normal progress of meiosis.

Animals↗
PubMed1967

Unilateral fixation of the ossicular chain associated with G-group chromosome deletion.

Unilateral fixation of the middle ear ossicles and possible delayed pubescence were associated with a short-arm deletion of one of the G-group chromosomes in a 15-year-old Negro girl. A similar chromosomal abnormality was found in the mother and three of six siblings without any clinical evidence of middle ear disease. The association of G-group deletions with other hereditary disease of bone suggests, however, that a pathogenic relationship may exist between them.

Adolescent↗