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G Jonson

Publications and source records attributed to G Jonson.

At least 37 records · Page 2Linked to original sources
PubMed1989

Expression and detection of different biotype-associated cell-bound haemagglutinins of Vibrio cholerae O1.

The expression of two cell-bound haemagglutinins, one sensitive to L-fucose (FSHA) and the other to D-mannose (MSHA), on Vibrio cholerae O1 strains of both the classical and the El Tor biotypes was studied by (i) agglutination of chicken and human group O erythrocytes in the presence of L-fucose or D-mannose, (ii) binding of the bacteria to L-fucose- and D-mannose-coated agarose beads, and (iii) agglutination of the bacteria by 'biotype-specific' antisera. All of the 12 classical strains studied that were isolated before 1979 gave FSHA of human O erythrocytes whereas only 6 of 17 classical strains isolated during recent epidemics expressed FSHA; a few of the classical strains expressed MSHA in addition to FSHA. All the El Tor strains gave MSHA of chicken erythrocytes and one strain also expressed FSHA. Both the cell-bound HAs were optimally expressed during the exponential phase of growth; FSHA markedly decreased during the late exponential phase while the MSHA usually persisted into the stationary phase. The expression of FSHA and MSHA correlated very well with the direct binding of vibrios to fucose- and mannose-coated agarose beads, respectively. Antiserum 'specific' for classical vibrios agglutinated classical strains expressing FSHA and also the El Tor strain exhibiting FSHA. Similarly, the anti-El Tor serum agglutinated all El Tor strains and also classical strains expressing MSHA, suggesting that the 'biotype-specific' sera were specific for the biotype-associated cell-bound HAs.

Animals↗
PubMed1989

Vibrio cholerae expresses cell surface antigens during intestinal infection which are not expressed during in vitro culture.

Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. At the same time, several of the env proteins ordinarily expressed in vitro had disappeared or become much reduced. The infection-induced novel env protein were immunogenic. In immunoblot analyses, antisera raised against in vivo-grown vibrios and then absorbed with in vitro-grown bacteria of the same strain specifically stained at least eight infection-induced antigens ranging from 62 to approximately 200 kilodaltons; absorption with washed in vivo-grown bacteria, on the other hand, removed the antibodies reacting with these antigens, indicating that the antigens were present on the bacterial cell surface. Conversely, antiserum against in vitro-grown bacteria reacted with several env antigens in in vitro-grown bacteria that were missing in the infection-derived vibrios. These adaptational changes were strikingly similar for different strains of cholera vibrios of both classical and El Tor biotypes. Most of the in vivo-specific proteins (with apparent molecular masses of approximately 200, approximately 150, approximately 140, 92, 68, 62, 43, and 29 kilodaltons) were not induced during cultivation of bacteria in iron-depleted medium and are probably not related to the iron-regulated env proteins known to be involved in iron transport systems.

Animals↗
PubMed1988

The maltose-inducible 43 kDa major outer membrane protein in Vibrio cholerae is immunogenic and common to different isolates.

We have previously demonstrated the presence of a maltose-inducible major outer membrane protein of 43 kDa in an El Tor Inaba strain of Vibrio cholerae. The occurrence of similar proteins was examined in several isolates of V. cholerae 01. The results indicate that the 43 kDa protein is common to all of the isolates as evidenced by Western blotting analysis with antiserum raised against this protein. The 43 kDa protein was maltose-inducible in most isolates although some strains exhibited a constitutive production of the protein. This protein was present also on V. cholerae 01 organisms harvested directly from the small intestine of rabbits with experimental cholera and it gave rise to specific antibodies after immunization with in vivo grown vibrios.

Animals↗
PubMed1984

Value of serum-5-nucleotidase, alkaline phosphatase and gamma-glutamyl transferase for prediction of liver metastases preoperatively in colorectal cancer.

In 133 patients laparotomized for colorectal cancer the serum-5-nucleotidase, alkaline phosphatase and gamma-glutamyltransferase were analysed preoperatively. The presence of liver metastases was established at laparotomy by palpation (prevalence 19%). The serum enzyme levels were elevated in 10-18% of patients without liver metastases and in 48-64% of patients with liver metastases. A comparison of the estimated tumor volume in the liver and the serum enzyme levels was performed. The predictive values of the three tests were computed at different reference limits. It was concluded that none of the tests used had any advantage of the other. To increase the diagnostic yield another reference limit than the normal at the laboratory can be used.

5'-Nucleotidase↗
PubMed1977

Chronic relapsing pancreatitis in a child--endoscopic diagnosis. A case report.

A case of idiopathic, chronic relapsing pancreatitis in a 14-year-old boy is described. With the help of endoscopic, retrograde choledocho-pancreaticography (ERCP), the final diagnosis was made. The boy has been free from symptoms two years, after a pancreatico-jejunostomy side-to-side. The etiology, incidence, diagnostics and treatment of this rare disease are discussed.

Adolescent↗