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Biomedical subjects

G K Steigleder

Publications and source records attributed to G K Steigleder.

At least 19 recordsLinked to original sources
PubMed1991

PIXE analysis in uninvolved skin of atopic patients and aged skin.

PIXE (proton-induced X-ray emission) analysis was used to determine the elemental distribution in normal-appearing skin of patients suffering from atopic eczema and in the skin of elderly people. With this technique, elements with atomic numbers greater than or equal to 14 can be detected simultaneously in cryosections of skin biopsies down to a concentration of 1 ppm. Compared with a control group, the epidermal concentrations of Zn and Cu, which are constituent parts of a variety of enzymes, were increased in uninvolved skin of patients with atopic eczema. An increased concentration of these two metals might indicate that even in the epidermis of clinically normal skin of atopic patients, the content of certain enzymes is increased. In the epidermis of elderly people the level of K was lower and that of Ca was higher than in the epidermis of a younger age group. The decreased K level may reflect a reduction of the intracellular volume in the epidermis of aged skin. As high Ca concentrations inhibit the proliferation of and promote the differentiation of keratinocytes, elevated Ca levels may be of importance for the age-associated decrease in epidermal turnover rate.

Adult↗
PubMed1990

[Is involvement of skin appendages or thickness of the epidermis an aid in differential diagnosis between lentigo maligna melanoma and superficially spreading melanoma in situ?].

Neither the involvement of hairfollicles nor of the ducts of the sweat glands by melanoma cells nor the thickness of the epidermis are helpful in differential diagnosis of lentigo maligna melanoma and superficial spreading melanoma in situ. The epidermis was thinner in LMM, however, LLM is mostly localized in the face where the epidermis is thinner than in other areas of the integument.

Biopsy↗
PubMed1989

[Cytokinetics and keratins of keratinocytes from skin of the elderly].

Regarding the keratin pattern of non-exposed skin, we found no significant qualitative or quantitative differences between 6 old persons (mean age 85 years) and 4 young adults (mean age 20 years). There was, however, a slight increase of proliferation keratins (K6, K16) in aged skin. In non-exposed skin taken from 6 old (mean age 70 years) and 5 young persons (mean age 37 years), longterm primary submersion cultures of keratinocytes did not show any significant differences as far as the classical parameters of growth behavior were concerned (i.e. plating efficiency, cell count, and labeled thymidine incorporation). In accordance with these findings, daily measurements of the thymidine kinase activity in the supernatants revealed discrete but not significant differences between keratinocytes in aged people and those in young persons.

Adolescent↗
PubMed1989

[Malignant melanoma and HIV infection].

Among 1000 patients with HIV infection consecutively examined at our dermatological department during the last 3 years, we diagnosed 4 cases of malignant melanoma. This figure is clearly higher than the statistical incidence of 4-13 cases per 100.000 people a year observed in West Germany. The mean age of our 4 patients and 3 additional cases reported in the literature is 10 years less than the average age found in melanoma patients without HIV infection. These findings indicate an increased risk of malignant melanoma in patients with HIV infection. In most of our patients, the cellular immunity was only marginally impaired, corresponding to the stages WR1 and WR2 (Walter Reed classification). Tumor formation, therefore, does apparently not depend on the severity of decompensation of the immunity.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Epidermal cell cultures--significance for wound coverage in the human].

Epithelial sheets can be cultivated from isolated epidermal cells; in this way, it is possible to increase the cell number considerably. H. Green and co-workers were the first to make use of such epithelia for the autologous covering of burn wounds. We modified this method and report on our experiences with this technique in a patient with small skin defects.

Adult↗
PubMed1979

[Insect stings].

Explore the source record for details and available documents.

Anaphylaxis↗
PubMed1979

[Beneficial effect of oral retinoid in psoriatic hyperproliferation. Autoradiographic studies on involved and non involved skin (author's transl)].

In 16 patients with generalized psoriasis treated with oral retinoid (Ro 10-9359; 1 mg/kg body weight/(day) autoradiographic studies were performed before, and 24 h, 48 h, 1 week and 3 weeks after beginning of treatment on involved and non involved epidermis. During the first week no significant changes of all cellular parameters were found. However, after 3 weeks of continuing medication the increased 3H-thymidine labelling index was markedly lowered and the prolonged DNA synthesis time was shortened; whereas, the cell cycle time remained unaffected. The findings revealed that oral retinoid has a distinct influence on epidermal cell proliferation towards normalization in both involved and non involved epidermis in psoriasis. The time needed for this effect indicates that the drug does not directly interfere with the cell cycle. It seems reasonable, therefore, to combine oral retinoids as a basic adjuvant drug with other antipsoriatic techniques, such as retinoid + anthralin, retinoid + PUVA, retinoid + UVB.

Cell Cycle↗
PubMed1979

[Retinoid-selective ultraviolet phototherapy (SUP) in psoriasis].

27 patients with generalized psoriasis were treated with oral administration of Ro 10-9359 (50--75 mg/d) and daily radiations with a source emitting UVA and UVB light at 292.5--335 nm (so called selective UV-phototherapy, SUP). In 24 patients full remission was seen, partly with few remaining lesions at the predilection sites. The average number of sessions necessary for clearing was 21.6 in 5.3 weeks. The total energy required was 68 J/cm2. Only slight side effects, such as cheilitis and hair loss (4 cases) were seen. The clinical efficacy of this combined retinoid-UV-treatment is comparable to the therapeutic effect of systemic PUVA; however, its practicability seems to be higher, and its application on an out-patient basis better.

Carotenoids↗
PubMed1979

In vitro examination of cell proliferation in dermatofibroma and in the overlying epidermis.

In nine typical cases of dermatofibroma autoradiographic methods were used to examine the proliferative activity in the tumor and in the overlying epidermis. The number of DNA-synthesizing cells in the dermal nodular lesions was extremely low (0 -- 0.1%). In seven of nine skin lesions acanthosis was to be seen in the overlying epidermis. The quantity of DNA-synthesizing cells in the epidermis overlying the dermatofibroma was not related to the thickness of the epidermis and remained independent, whether the predominant element in the tumor is fibrillar or cellular. In this study we were able to demonstrate the existence of a direct relation between the degree of epidermal proliferation and the distance between the tumor and the epidermis. The duration of DNA-synthesis was constant.

Cell Division↗
PubMed1979

Acid nonspecific esterase in human Langerhans cells.

Epidermal dendritic cells showing a diffuse activity of acid nonspecific esterase were observed in three cases of mycosis fungoides stage I. As these cells by their appearance and localization resemble Langerhans cells, and dermal histiocytes and macrophages react in the same manner, this is another enzyme cytochemical suggestion of a relationship between Langerhans cells and the monocyte-macrophage-system.

Carboxylic Ester Hydrolases↗
PubMed1979

Cell proliferation in psoriatics.

In psoriasis the DNA synthesis time (ts) of keratinocytes in characteristically lengthened in unaffected skin, and in very early and also fully developed lesions. The prolongation of ts in unaffected psoriatic skin can be increased significantly by removal of the horny layer with adhesive tape. After the same procedure, ts remains unchanged in healthy skin and becomes shortened in the skin of patients suffering from atopic dermatitis. These variations of ts after a standardized irritation indicate that this phenomenon may be used in diagnosis of latent psoriasis.

Cell Division↗
PubMed1979

Oral retinoid and UVB radiation: a new, alternative treatment for psoriasis on an out-patient basis.

The combined application of an oral retinoid (Ro 10-9359) and phototherapy with predominantly UVB radiation (Selective Ultraviolet Phototherapy=SUP) is a new, highly effective method of treating psoriasis. It has few side effects and can be performed on an out-patient basis. With the aid of this combined treatment we achieved good or very good improvement in 19 out of 23 patients with generalized psoriasis (=82.6%). The average number of radiation sessions required to achieve this was 22.9, and the mean total therapeutic dose (TTD) was 73 J/cm2. In a control group of 40 psoriasis patients, who received only radiation therapy, we achieved good or very good results in only 60% with an average of 26 radiation sessions and 94 J/cm2 TTD. The effect of the oral retinoid and UVB radiation therapy is apparently additive, since the retinoid does not increase the sensitivity of the skin to light.

Administration, Oral↗