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Biomedical subjects

G K Yamamoto

Publications and source records attributed to G K Yamamoto.

5 recordsLinked to original sources
PubMed1979

Complement in tears from normal humans.

Tears from ten normal persons were used in hemolytic assays to test for activity of total hemolytic complement (tested in all ten subjects), activities of each of the nine complement components (tested in two subjects), and activity of the alternate pathway (tested in two subjects). A modified radial-immunodiffusion method was used to confirm the presence of C3 and C4 complement proteins in the tear samples from all ten subjects. Factor B protein was detected in tears from two of four normal subjects tested. In dilutions up to 1:4, hemolytic activity was observed in tears from five of the ten subjects. Samples from the remaining five subjects had CH50 activity at dilutions up to 1:2. In control tests, human serum had CH50 activity at 1:32. No lysis occurred in Veronal-buffered saline or in serum and tears heated to 56 degrees C for 30 minutes. Complement proteins C3 through C9 displayed hemolytic activity in tears from each of two subjects tested for complement components. The classic and alternate complement pathways in tears can be included among the defense mechanisms of the ocular surfaces.

Complement C3↗
PubMed1979

Fungal invasion of a therapeutic soft contact lens and cornea.

A fungal corneal infection occurred in a 66-year-old man who wore a therapeutic soft contact lens for 12 months during treatment for a metaherpetic corneal epithelial erosion. The infection was documented by finding positive cultures from both the contact lens and the cornea, and histologic evidence of fungal infiltration of the therapeutic soft lens. The fungus was identified as Cephalosporium acremonium. Pathogenic fungal invasion of soft lenses is unusual. Corneal infections associated with such conditions are rare. This case demonstrates histologically, a pathogenic fungal corneal infection arising from therapeutic contact lens wear. Factors that may influence soft lens infiltration by fungi are: (1) enzymatic activity produced by the fungus, and (2) lens material properties which provide a matrix and a nutrient source for fungal growth. Continuous-wear soft contact lens treatment with topical steroid and prophylactic antibiotics used in combination in an already compromised cornea were thought to be responsible in an already compromised cornea were thought to be responsible for this complication. A therapeutic penetrating keratoplasty was performed when the infection and its accompanying inflammation became clinically unresponsive to multifold therapy, and a corneal perforation was imminent. The eye was salvaged with a resulting clear graft and stable intraocular pressures.

Acremonium↗
PubMed1979

IgG in human cornea stained by various techniques.

Four eyes were divided into portions which were prepared by various techniques for immunofluorescence microscopy. One portion was frozen and processed by cryostat cutting, a second was fixed in alcohol-acetic acid and embedded in paraffin, and a third was soaked in saline for 2 days to elute IgG and was then processed by the alcohol-acetic acid technique. All tissues received stain for IgG by either the direct (one-layer) or indirect (two-layer) technique. In all instances IgG was demonstrated in the same areas of the cornea. We conclude that the presence of IgG in human cornea is not dependent on the method used for fixation or processing of the tissue.

Aged↗
PubMedDate not supplied

Long-term ocular changes in cystinosis: observations in renal transplant recipients.

Following renal transplantation in nephropathic cystinosis, an extension of retinopathy associated with a variety of subtle functional ocular abnormalities may occur. However, these abnormalities have not (except for photophobia) been of subjective significance to cystinotic patients. Our data, gathered from patients transplanted as long as nine years ago, indicate that cystinotic patients should not have renal transplant withheld because of the fear of subsequent progressive ocular deterioration.

Adolescent↗