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Biomedical subjects

G Kálmán

Publications and source records attributed to G Kálmán.

18 recordsLinked to original sources

Novel approach of corn fiber utilization.

The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, and 14% protein. Extraction of the highly valuable, cholesterol-lowering corn fiber oil is not feasible owing to its low (2% w/w) concentration in the fiber. The developed technology is based on simple and inexpensive procedures, like washing with hot water, dilute acid hydrolysis at 120 degrees C, enzymatic hydrolysis of cellulose, screening, drying, and extraction. The main fractions are sharply separated in the order of starch, hemicellulose, cellulose, lipoprotein, and lignin). The lipoprotein fraction adds up to 10% of the original dry corn fiber, and contains 45% corn fiber oil, thus yielding more oil than direct extraction of the fiber. It is concluded that the defined method makes the extraction of the corn fiber oil economically feasible. The fractionation process also significantly increases the yield of cholesterol-lowering substances (sterols and sterolesters). At the same time clear and utilizable fractions of monosaccharides, protein, and lignin are produced.

Cellulose↗

Axotomy, axocompression, axocontusion: effects of mechanical injuries on the catecholamine content of unmyelinated syC fibers.

Authors studied the effects of transection (axotomy) and crush of the rat sciatic nerve, by means of De La Torrés glyoxalic acid fluorescence histochemical technique. It was shown that a simple nerve crush (axocompression) did not induce any major change in the number and fluorescence intensity of unmyelinated axons in the distal stump of the sciatic nerve, even though myelinated axons underwent Wallerian degeneration. By contrast, axotomy resulted in a complete cessation of catecholamine-induced fluorescence; similar effect was exerted by an intense, controlled crush (axocontusion). Supposing that not only postganglionic (syC) but also sensory (drC) unmyelinated axons undergo similar alterations after various mechanical injuries, the apparently controversial results regarding the effects of axotomy and nerve crush, in producing transganglionally mediated depletion of marker substances in the upper dorsal horn, can easily be reconciled.

Animals↗

Development of the autonomic ground plexus in the atrioventricular valves of the rat.

The development of the adrenergic and cholinergic innervation of the rat atrioventricular valves was studied in whole mount stretch preparations. Specimens obtained from rats ageing 2-30 days were processed for the histofluorescence and enzyme histochemical demonstration of monoamines and acetylcholinesterase (AChE) activity, respectively. Adrenergic and AChE-positive nerve fibres could be detected from the 9-10th postnatal days onwards. Fluorescence microscopy showed the presence of many brightly fluorescent mast cells in the close vicinity of the ingrowing terminals. The presence of mast cells during early stages of development of the ground plexus may be related either to the preneural state of the tissue or the structural and/or functional maturation of the autonomic nerve terminals.

Acetylcholinesterase↗

[The role of mastocytes in the development of the adrenergic innervation of the iris].

During the development of the primary adrenerg-plexus in the rat iris presence of mastocytes showing intensive fluorescence can be observed using the method of Falck. After the formation of the primary plexus this phenomenon disappears. By electron microscopy in the early period of the postnatal life authors demonstrated the presence of mastocytes closely related to developing neurofibrils and Schwann-cells. In these mastocytes signs of degranulation were to be observed. On the base of the latter finding one can assume, that substances deliberated from the granules may play a part in the regulation of the development of innervation.

Animals↗

The role of mast cells in the development of adrenergic innervation in the rat iris.

In the rat iris, during the development of the adrenergic ground plexus, intensely fluorescent mast cells, not observable after maturation of the same, could be revealed with the Falck technique. According to the electron microscopic investigations the mast cells are in close connection with the developing nerve fibres and Schwann cells in the early stage of postnatal life. On the basis of signs suggesting degranulation it is concluded that the mast cells can play a role in the regulation of the development of innervation by means of substances released from their granules.

Adrenergic Fibers↗