Biomedical subjects
Publications and source records attributed to G Kai.
The AAc grafted porous PC film was prepared by simultaneous UV irradiation and the pH sensitivity of the grafted films was investigated with the simple controlled release device designed by the authors. The grafted porous PC films showed remarkable pH sensitivity and can be reused after proper treatment.
In this study, a continuous-flow UV-induced mutation (CUM) device and the CUM device coupled to a selector (CUMS) reactor were fabricated and tested for their ability to enhance the probability of obtaining populations capable of chlorinated organic biodegradation. A mixed culture of bacteria were used as the starting strain for both the CUM and CUMS processes. Populations were obtained from the CUM and CUMS systems capable of 4-chlorobenzoic acid, 2,4-dichlorobenzoic acid and chlorendic acid biodegradation. Non-UV irradiated population served as controls for the experiments and did not demonstrate chlorinated organic biodegradation over the test duration.
A continuous-flow UV-induced mutation device which incorporates starting strain cultivation, UV irradiation and mutant reproduction was conceptualized and tested in this study using streptomycin resistance as an indicator of mutant production. For the experimental conditions employed and populations used, the mutation frequency for streptomycin resistance ranged from 10(-4) to 10(-5) cfu/ml. These mutation frequencies are comparable with conventional batch UV mutation methods and represent a gain of 3 orders of magnitude over the spontaneous mutation frequency.