Biomedical subjects

G Kajimoto

Publications and source records attributed to G Kajimoto.

3 recordsLinked to original sources

PubMed1990

Novel pathways of oleic and cis-vaccenic acid biosynthesis by an enzymatic double-bond shifting reaction in higher plants.

The novel pathways of oleic acid formation from cis-vaccenic (cis-11-octadecenoic) acid and of cis-vaccenic acid formation from oleic acid by enzymatic positional isomerization have been proposed in higher plants, based on stable-isotope experiments using [2,2-2H2]cis-vaccenate or [2,2-2H2]oleate as an immediate precursor. A pulp homogenate and also pulp slices prepared from developing kaki (Diospyros kaki) fruit could catalyze these hitherto unknown isomerizations. This suggests the presence of a new type of isomerase responsible for the double-bond shifting reaction without cis-trans isomerization in the middle of fatty acid carbon chains.

Chemical Phenomena↗

PubMed1989

Effect of dietary vitamin E on the toxicity of autoxidized oil to rats.

Tocopherol, one of the antioxidants in vegetable fats and oils, was added to the autoxidized diet of male rats at the same tocopherol level as soybean oil before autoxidation. Autoxidized soybean oil was used as the test lipid; fresh soybean oil was the control. The diet containing autoxidized oil depressed the body weight gains and increased the tissue ratios to body weight, with the exception of testicle. Parallel with those, increased hemolysis, peroxide value and carbonyl value in the tissue lipids, and a decrease in linoleic or arachidonic acid was observed. The effect of dietary vitamin E on the tissue lipid peroxidation was different among the individual organs. From the results obtained by chemical analyses, the lipid peroxidation caused in the liver, heart and intestine was recognized to be suppressed effectively when the same tocopherol level as fresh soybean oil was added to the diet.

Animals↗

PubMed1986

Decrease of NADH in yeast cells by external ferricyanide reduction.

Ferricyanide reduction catalyzed by vitamin K-3 was accompanied by the decrease in intracellular (NAD(P)H concentration of yeast cells, and the rate of ferricyanide reduction depended on intracellular concentration of NADH rather than NADPH. The addition of glucose to the cell suspensions enhanced both ferricyanide reduction and intracellular NADH concentration. The catalytic action of vitamin K-3 on ferricyanide reduction was observed in the presence of NADH and plasma membrane preparations. As the toxic action of vitamin K-3 on cell growth of yeast was enhanced by addition of ferricyanide, ferricyanide reduction catalyzed by vitamin K-3 may inhibit cell growth by decreasing intracellular NADH concentration.

Ferricyanides↗