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Biomedical subjects

G Kalev

Publications and source records attributed to G Kalev.

7 recordsLinked to original sources
PubMed1975

[Characteristics of bull semen].

Studied were comparatively some morphologic, biochemical, and biological methods employed to characterize the biologic value of bull's semen. Particular attention was paid to the objective methods of evaluation. Reflected is the experience gained in this country as well as the author's personal experience in characterizing the ejaculates and storing 24-72 H semen at 0-4 degrees C and continuous storing at ultralow temperatures. Given are also the theoretic achievements on the problem, and a stress is laid on the applicability of the methods for evaluating the semen in the artificial insemination practice with cows.

Animals↗
PubMed1975

[Comparative studies of some methods of freezing bull sperm].

Comparative studies were carried out with a total of 27 ejaculates obtained from 20 bulls to establish the alleged advantages of some of the tested technological methods for freezing the semen in the form of pellets, ampules, straws, and minitubes. All four methods showed comparable results. The morphologic changes in the thawed semen, resp., spermatozoa varied within close limits--from 41 up to 42.5 per cent of the cells had secondary defects. So far as the extent of contamination was concerned the investigations showed that the four methods should be improved in terms of the hygiene standard in the collection and handling of semen, especially in the granulation of semen and its storage in the form of pellets.

Animals↗
PubMed1975

[Complex-forming factors in the dilution of bull sperm for deep freezing].

Micro- and ultramicroelectrophoresis on Cellogel were employed to study the formation of complexes of bull's semen and the constituents of Nagaze-Niva's extender in all possible combinations. It was found that a new glycoprotein complex is formed in mixing the semen with yolk. This complex was found to bind 82 +/- 1.5 per cent of all proteins in the sample. The addition of lactose to the yolk-diluted semen was shown to lead to the dispersion of this complex into 4--5 subunits. The cation fraction of the semen plasma and glycerin were established to take no part in the complex formation. Discussed are the problems associated with the dispersion effect of lactose and the higher (twice to three times) survival rate of semen trated with extenders containing saccharides.

Animals↗