Biomedical subjects

G Kh Georgiev

Publications and source records attributed to G Kh Georgiev.

5 recordsLinked to original sources

PubMed1986

[Effect of cryopreservation on the acrosomal enzyme activity of the spermatozoa of rams].

Studied was the effect of cryoconservation on the activity of acrosine and acrosomal acid phosphatase of spermatozoa of rams stored in the form of pellets with the use of a diluent after Nagaze-Niva. It was found that the activity of acid phosphatase dropped by 35 per cent when stored in the course of 24 hours and 7 days. Acrosine was shown to be more stable at cryoconservation--its activity did not change under the same conditions of storing, however, it dropped by 30 per cent for 7 days. Admittedly, one of the reasons for the drop of the fertilizing capacity of ram spermatozoa after freezing was the drop of acrosomal enzyme activity. Most probably, the noxious factors were two--ultralow temperatures, and storage duration of spermatozoa.

Acid Phosphatase↗

PubMed1983

[Activity and molecular forms of nicotinamide adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent malate dehydrogenase in bull spermatozoa].

Investigations were carried out on the activity and the isoenzyme composition of the NAD- and NADP-dependent malatedehydrogenase in the soluble and sedimental proteins of bull spermatozoa. It was found that the activity of the NAD-dependent malatedehydrogenase was higher than the activity of the NADP-dependent one both in the soluble and in the sedimental proteins. The difference in the activity was spectrophotometrically and electrophoretically demonstrated.

Animals↗

PubMed1983

[Acrosome--its protein composition and function].

In situ studies on total proteins and some hydrolytic enzymes extracted by acid treatment of the bull sperm acrosomes were carried out. The acrosomal proteins studied by cathodic disc electrophoresis (pH 4.3) were found to be polymorphic, with three phenotypes. The isoenzyme pattern of the acrosomal enzymes under study (acrosin, acid phosphatase and non-specific esterases) was identified as well.

Acid Phosphatase↗

PubMed1977

[Activity and isoenzyme pattern of alkaline and acid phosphatases in bull seminal plasma].

Investigations are carried out on the activity and isozyme pattern of the alkaline and acid phosphatases in bull's seminal plasma. To determine the activity of the two phosphatases, two different colorimetric methods are used. The electrophoretic separation of the isozymes of the acid and alkaline phosphatases is performed on polyacrylamide and starch gels.

Acid Phosphatase↗

PubMed1975

[Chloride levels in the estral secretion and the fertility of cows].

Studied was the chloride content of the estral secretion of cows at their insemination. It was found that chlorides vary from 351 to 1130 mg% on an average. In 90 per cent of the cows the chloride content varied within the 701-1200 mg% range. There is certain correlation between the chloride content and the conception rate of cows. The latter had highest values in cases where chlorides ranged from 701 to 800 mg%.

Animals↗