Biomedical subjects
Publications and source records attributed to G Kochetov.
Baker's yeast transketolase inactivated by tetranitromethane was digested with Staphylococcus aureus V8 protease. Four peptides absorbing at 360 nm were isolated by reverse-phase HPLC and sequenced. The modified tyrosines were identified as Tyr-184, Tyr-210 and Tyr-370.
The multiplicity of transketolase forms, differing in thermostability and separated by phosphocellulose chromatography, has been shown. Using SDS-PAGE, it was found that the mobility of yeast enzyme forms in all cases was identical. Certain forms of transketolase from yeast, rat or pig liver and from some organs of the rabbit were similar with regard to their chromatographic behaviour and thermostability. The form ratio seems to be determined by the physiological state of the organism.