Biomedical subjects

G Konwalinka

Publications and source records attributed to G Konwalinka.

77 records · Page 5Linked to original sources

PubMed1981

A micro-culture system for cloning human T lymphocytes in agar.

A simple and reproducible single-layer micro-agar culture system for cloning of human T lymphocytes has been described. The system consists of an agar layer, in which mononuclear cells from peripheral blood were suspended, and a liquid overlayer containing the mitogenic substance. The advantages of the described method are a low incubation volume (0.5 ml) and the liquid overlayer. The addition of different test substances to the liquid overlayer is simple and easily controllable. Depending on the agar concentration a different number of formed colonies can be found floating in the liquid phase. The morphological, cytochemical and immunological characteristics of the cells from those aggregates could be easily studied. The T-cell characteristics of formed clusters and colonies was confirmed by immunofluorescence and E rosette formation. The effects of agar, serum and cell concentrations, as well as the mitogenic activation caused by three lectins on the development and number of colonies were studied on day 7, 10 and 14 of incubation.

Clone Cells↗

PubMed1980

A new approach to the morphological and cytochemical evaluation of human bone marrow CFUC in agar cultures.

A simple procedure is described for the routine morphological examination of different types of colonies and clusters in agar cultures. The following cytochemical methods are used for the identification of cells: naphthol-ASD-chloroacetate esterase, naphthol-ASD-acetate esterase with and without addition of sodium fluoride, Biebrich scarlet and acid phosphatase. Based on their cytochemical staining properties neutrophils, eosinophils, monocytes and macrophages can be readily identified in their different stages of maturation and an assessment made of the cellular composition of colonies and clusters.

Agar↗

PubMed1979

[Bone marrow culture in aplastic anemia and preleukemias].

After incubation of bone marrow cells obtained from normal subjects and patients with pancytopenia in the double layer agar culture (Robinson-Pike), the clusters of different size and the colonies were counted on days 2, 3, 5, 7 and 10. Aplastic anemia and two types of preleukemia could be distinguished by a different growth pattern. A high incidence of small clusters (4-12 cells) was found in patients with aplastic anemia on days 2 and 3, whereas these aggregates were markedly reduced in preleukemia of type B. In preleukemia of type A an excessive number of small clusters was obtained. The morphology of cells in clusters and colonies was studied by cytochemical staining and electronmicroscopy. In preleukemia marked defects of maturation were demonstrable in the cells.

Anemia, Aplastic↗

PubMed1979

[Aplastic anemias].

In the first part of this review diagnostic criteria and prognostic factors are summarized. The results of bone marrow culture with particular reference to the pathophysiology of this disease, are discussed. Finally, the conventional therapeutic approaches are summarized and recent developments in experimental treatment modalities are discussed.

Anemia, Aplastic↗

PubMed1978

Detection of cytochemical and morphological anomalies in 'preleukemia'.

The present investigations confirm and extend previous reports on the occurrence of cytochemical anomalies among the hemopoietic cells in preleukemia. The cytochemical patterns of the hemopietic cells obtained from 19 patients suffering from a preleukemic disorder have been studied. The diagnostic value of the cytochemical examination using a panel of 10 different tests is emphasized. In a prospective study in 17 out of 25 patients, preleukemia had been correctly diagnosed. In 4 patients leukemia developed within 2-4 months from the diagnosis ('imminent leukemia'), in 13 patients leukemia or smouldering leukemia developed between 4 and 25 months after the diagnosis ('true preleukemia'). The cytochemical profiles of the hemopoietic cells of the latter have been compared with those of 14 patients suffering from aplastic anemia. In preleukemia the blasts did not exceed 3% of the nucleated bone marrow cells. The clinical value of the diagnosis 'preleukemia' is discussed as well as the prognosis and the possible therapeutic approaches.

Adult↗