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Biomedical subjects

G Kröning

Publications and source records attributed to G Kröning.

14 recordsLinked to original sources
PubMed1990

Prospective study on the influence of radiochemotherapy on pituitary function in children with acute leukaemia and NHL.

To assess effects of chemo- and radiotherapy on the endocrine system 31 children with acute leukaemia and NHL (3 AML, 24 ALL, 4 NHL) were investigated. Children were treated according to modified BFM protocols. 25 patients were before, 5 during and one after puberty (2 to 16 y.). Before treatment, during induction therapy, during cranial irradiation, 4-6 weeks later and during maintenance therapy the following hormone values were estimated: TSH and prolactin basal and 30 min. after TRH (5 micrograms/kg i.v.), LH and FSH basal. Final investigations included total T4 and T3. In conclusion, chemo- und radiotherapy lead to transient elevations of TSH and prolactin in a few patients, but without proof for permanent disorders. Due to the fact all 3 patients with hyperprolactinaemia showed high prolactin levels (700 to 770 mU/l) already before treatment it is unlikely therapy was the main cause of these observed alterations. Although basal LH and FSH values were in normal ranges for age the increasing values after cranial irradiation in prepubertal children may reflect a possible initiation of early maturation, reported by others. Furthermore a retrospective growth study was performed in children treated with 2 different protocols. Protocol LSA2L2 used in the past before 1981 resulted in a permanent reduction of the height. In contrast, the mean SDS for height in children treated with protocol VII declined only during the intensive period of treatment. A catch-up growth occured already during maintenance therapy. Prophylactic cranial irradiation with 18 Gy in our patients under protocol LSA2L2 did not affect growth during the first 5 years after diagnosis.

Acute Disease↗
PubMed1989

[A complex program for microcomputer-supported immunoassay analysis].

The increasing availability of analytical methods based on saturation analysis call for efficient assay calculation. A comprehensive computer program was developed, allowing the calculation of different kinds of assays. Due to the user friendly structure it can be used also by inexperienced laboratory staff without difficulties. Its large adaptability can be a usefull support to optimize various kinds of assays.

Immunoassay↗
PubMed1988

[Effects of dithiocarbamates on the mitotic activity of thymocytes].

The effects of dithiocarbamates on the mitotic activity of thymocytes were studied in vitro and in vivo. The mitotic activity was determined by counting of the metaphases blocked by colchicine. Using thymocytes of mice the mitotic activity in short-time cultures was inhibited (50%) by the following concentrations of the substances under study (EC50-values in mol/l): tetraethylthiuramdisulfide; (1; disulfiram) 4.2.10(-9); diethyldithiocarbamate (2) 3.2.10(-8), Cu-2, 2.6.10(-8), Zn-dimethyldithiocarbamate (3; Ziram) 1.9.10(-8), Zn-ethylene-bis-dithiocarbamate (4; Zineb) 2.3.10(-6). Using rat and guinea-pig thymocytes the ED50- values for 1 and 2 were found in the same concentration range as compared with thymocytes of mice. There was no impact on the vitality of cells by the mitosis inhibiting concentrations. In vivo the mitotic activity of thymocytes in mice was decreased only after application of high doses (500 mg/kg i.p.) of 2. After depletion of reduced glutathione (GSH) by the pretreatment of mice with diethylmaleate the effects of 2 were increased in vivo. In vitro GSH (10(-4) mol/l) decreased the mitosis inhibiting activity of 2 in mice thymocytes and the inhibition of the SH-enzyme ecto-ATPase in rat thymocytes. It is supposed that the inhibition of mitosis is due to the reaction of dithiocarbamates with SH-groups of microtubules and other functionally important proteines. The formation of tiyl radicals from dithiocarbamates may play a certain role in redox processes which can be influenced by GSH.

Adenosine Triphosphatases↗