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Biomedical subjects

G Krishnan

Publications and source records attributed to G Krishnan.

At least 37 records · Page 2Linked to original sources
PubMed1990

Are B lymphocytes involved in allograft/tumor rejection and GVH?

Several earlier observations by us and other investigators led us to propose a thesis that B lymphocytes, when activated by a virus, allo-antigen or bacterial protein would enter into effector arm of the immune response and cause allograft and tumor rejection or GVH-type of lesion in immunosuppressed animals. These effector cells may act directly in the process by contact with other cells or liberating cytokines. Such an hypothesis needs further support from experiments involving cancer and transplantation patients' B cells.

Animals↗
PubMed1986

DNA sequence analysis: a procedure to find homologies among many sequences.

SEQCMP, a program that analyzes and searches for homology among multiple nucleic acid sequences, is described. The sequences are compared by the dot matrix method and the consensus sequence is derived by superimposing all the dot matrices on one another. The program is written in MBASIC and runs on IBM-PC microcomputer. It is interactive and can be used by investigators with no computer background or experience.

Base Sequence↗
PubMed1986

Purification and characterization of malignancy associated alpha-1 protein from lung tumor of a patient who died of primary carcinoma of the lung.

Malignancy associated ALPHA-1 protein(MAP) was isolated from a kilogram quantity of a lung tumor obtained at an autopsy of a patient who died of primary carcinoma of the lung. Acid extraction, Millipore filtration on a 30,000 molecular weight cut off membrane, treatment of the retentate with 50% saturated ammonium sulfate and gel filtration on Sephadex G-75 yielded a fairly pure material as judged by PAGE and RP-HPLC.

Carcinoma↗
PubMed1983

Radioimmunoassay for human prothrombin.

I describe a radioimmunoassay for human prothrombin, with use of a double-antibody technique. Antiserum raised in rabbits was absorbed with Al(OH)3 and heated to 56 degrees C for 30 min. 125I-labeled prothrombin retaining more than 90% of its biological activity was prepared by the iodine monochloride method. The mean concentration of prothrombin in plasma of 12 normal individuals was 100 +/- 29.4 mg/L (2 SD). Prothrombin values were somewhat lower than those obtained by the Laurell electroimmunoassay or by two-stage biological assay of the same plasma, done the same day. The biological values were converted to protein on the basis of 1960 int. units/mg by comparison with the other two assays. The ability of activation fragments of human prothrombin to inhibit binding of labeled prothrombin to its antibody was evaluated by competitive radioimmunoassay. Although precipitin lines formed with undiluted antiserum against all the fragments tested (F-1, F-1.2, prethrombin-1, and thrombin), none of the fragments competed well with prothrombin, even in 10-fold molar excess. Evidently, the structural integrity of the prothrombin molecule is essential for its maximum binding to the antiserum, and antigenic sites are lost during its activation.

Animals↗
PubMed1980

Similarity between tryptic peptides derived from equine mu and rabbit gamma chains of affinity labeled antilactose antibodies of restricted heterogeneity.

Equine IgM and rabbit IgG antilactose antibodies of restricted heterogeneity were affinity labeled with two different bromoacetyl lactose reagents. Tryptic peptides derived from the heavy chains of the antibodies were analyzed for their amino acid compositions. Surprisingly they were quite similar. The possible genetic implication of this interesting observation is briefly discussed.

Affinity Labels↗
PubMed1977

A placebo-controlled, double-blind trial of Benorylate tablets in the treatment of bursitis and synovitis due to sports injury.

In this two-week study, Benoral tablets, at a 4.5 g daily dosage were compared with a matching placebo in 20 patients suffering from sports injury. Four assessments were made: pain at rest, pain on movement, tenderness and soft tissue swelling. In each case the active treatment group (benorylate) was statistically greater than that in the placebo group at one week and after two weeks' treatment.

Athletic Injuries↗
PubMed1976

Oxprenolol in the treatment of examination stress.

In a double-blind preliminary study in 32 students exhibiting symptoms of examination stress, treatment with 80 mg oxprenolol daily or 4 mg diazepam daily were found to be equally effective in relieving anxiety and tension, as assessed by both students and physician. Although students on diazepam became significantly more confident of success than those on oxprenolol, their results were worse than expected. In contrast, students on oxprenolol did not gain in confidence and they were significantly more successful than anticipated by their tutors.

Adolescent↗
PubMed1976

Comparison of two concentrations of tretinoin solution in the topical treatment of acne vulgaris.

In patients with facial acne good results were obtained by topical treatment with a 0-05 per cent solution of retinoic-acid (tretinoin) solution and with a 0-025 per cent solution. The improvement was judged by clinical assessment and by counts of comedones, papules and pustules before and after the 12-week trial. Side-effects such as irritation and erythema were less with the 0-025 per cent solution. The use of swabs impregnated with a constant volume of solution and sealed in a sachet proved to be a convenient method of application. In the placebo group, swabs impregnated with the solvent only showed virtually no effect on facial acne.

Acne Vulgaris↗