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Biomedical subjects

G Kuśnierz-Alejska

Publications and source records attributed to G Kuśnierz-Alejska.

17 recordsLinked to original sources
PubMed1999

NOR polyagglutination and Sta glycophorin in one family: relation of NOR polyagglutination to terminal alpha-galactose residues and abnormal glycolipids.

BACKGROUND: This report describes the characterization of polyagglutinable red cells (RBCs), identified in two generations of a Polish family. CASE REPORT: Untreated and modified RBCs of the proposita (TS) were tested by serologic methods, using human sera, antibodies, lectins, and inhibitors of agglutination. Moreover, glycophorins were characterized by sodium docecyl sulfate-polyacrylamide gel electrophoresis and Western blotting, and glycolipids were purified, fractionated by thin-layer chromatography, and detected with Ricinus communis agglutinin I (RCA-I, specific for galactose residues) and Griffonia simplicifolia IB4 lectin (GSL-IB4, specific for Gal alpha1-3Gal- structure). Some of the experiments were also performed on RBCs of members of TS's family. RESULTS: Polyagglutination, found in four members of TS's family, was identified as the second case of an earlier described NOR polyagglutination. The polyagglutination was decreased by treating the RBCs with alpha-galactosidase and was inhibited by a neutral glycolipid fraction from NOR+ RBCs. Detection of neutral glycolipids of TS's RBCs on the thin-layer plate by RCA-I and GSL-IB4 revealed the presence of components that were not detectable in control RBCs. Moreover, Western blotting of RBC membranes from five family members with glycophorin monoclonal antibodies and agglutination assays with anti-St(a) and anti-Dantu sera identified the presence of St(a) glycophorin in four members of the family, two of whom were NOR+ and two NOR-. CONCLUSION: Our results showed that two rare features of TS's RBCs, NOR polyagglutination and St(a) glycophorin, are inherited independently, and that NOR+ RBCs contain neutral glycolipids with an abnormal oligosaccharide structure, most likely terminated with alpha-galactosyl residues.

Adult↗
PubMed1997

Degradation of glycophorin A of human erythrocytes in patients with myelo- or lymphoproliferative disorders: possible role of neutrophil proteases.

We have previously reported that glycophorin A (GPA) of human erythrocytes (carrying blood group M and N determinants) was totally digested by incubation of erythrocytes with human neutrophil elastase (HNE) and cathepsin G (CathG). The membrane-bound GPA fragments fractionated by SDS-PAGE gave characteristic patterns of bands detected by immunoblotting with the monoclonal antibody PEP80. Erythrocytes were incubated with HNE and CathG at low enzyme concentrations, similar to those found in vivo. Characteristic electrophoretic patterns of bands derived from a partial GPA digestion were observed and these patterns were different for both enzymes and different from those obtained after total GPA digestion. GPA was also partially digested by incubation of erythrocytes with granulocytes in the presence of Ca2+ and calcium ionophore and electrophoretic pattern of digestion products was similar to that obtained with low doses of HNE. No GPA digestion products were detected after treatment of erythrocytes with plasmin and kallikrein. Untreated erythrocytes of 21 patients with various myelo- or lymphoproliferative disorders were tested by SDS-PAGE of RBC membranes and immunoblotting with the anti-GPA PEP80 antibody. GPA degradation products, resembling those formed by a mild CathG treatment of control RBC, were detected in nine patients. GPA fragmentation was in some cases accompanied by a reduced expression of blood group MN determinants. No distinct relation was observed between the occurrence of GPA degradation in erythrocytes and increases in plasma concentrations of HNE-alpha1-proteinase inhibitor (alpha1-PI) complex considered to be an indication of a release of neutrophil proteinases in vivo. However, the results suggested that a partial GPA degradation in haematological proliferative disorders may occur due to limited proteolysis by neutrophil proteinases, most likely by CathG.

Adolescent↗
PubMed1996

[RHnull syndrome--diagnostic and therapeutic problems].

A very rare Rhnull phenotype as a cause of haemolytic anaemia in a female patients was for the first time detected in Poland. The beneficial influence of splenectomy was demonstrated. It was proved that a single transfusion of Rh negative red cell concentrate, because of the lack of Rhnull blood, did not cause any acute adverse reactions. However, a transient, subclinical delayed haemolytic reaction due to the stimulation of anti-c and anti-e was observed.

Aged↗
PubMed1994

[Use of modified acid elution technique in laboratory diagnosis of autoimmune hemolytic anemia].

Three modifications of acid elution method were used for the diagnosis of autoimmune haemolytic anaemia. These procedures were adapted to obtain more potent eluates and to prepare intact antibody-free red blood cells for phenotyping and for autologous absorption of warm-reactive autoantibodies. From the results presented in this paper we conclude that the modifications of the acid elution method are better than the up to now used procedure with a mixture of proteolytic enzyme and a thiol reagent. Moreover, these procedures are quick and easy to perform as well as more efficient. The obtained colourless eluates can be investigated in a gel-lest.

Adult↗
PubMed1993

[Lan antigen of erythrocytes and clinical significance of anti-Lan antibody].

On the basis of the actual knowledge on Lan antigen, the first example of anti-Lan in Polish population was described. This alloantibody found in the serum of Lan negative mother was responsible for the haemolytic disease of her newborn. Family study revealed two Lan negative members: the father and the brother of the proposita. This paper emphasizes the importance of screening for immune alloantibodies, performed in the sera of all women independently of their ABO and Rh blood groups, at early period of pregnancy.

Adult↗
PubMed1993

[Use of microtest in gel for compatibility in serological testing before transfusion].

A gel technique for the detection of red blood cell antigen--antibody reaction was evaluated in pretransfusion compatibility tests. The evaluation was undertaken on 917 blood samples of recipients and 2450 samples of donors. Parallel study of microtest in gel and classical techniques revealed that gel technique was more sensitive than the classical one, because some antibodies of Rh, Kell and Kidd systems were found by this method exclusively. It is also more specific because a lot of unspecific reactions could be eliminated. Therefore, it was decided to use the microtest in gel for crossmatching of recipient serum against red cells from each intended donor--particularly when the patient had irregular erythrocyte antibodies. The results presented in this paper revealed that in gel is one of the most potent tests and it is the safest for routine use before transfusion.

Antigen-Antibody Reactions↗
PubMed1992

[Evaluation of microtest in dextran gel for blood group serology].

Microtest in gel was compared to the traditional, immunohaematological methods. Blood samples of 1.032 persons were tested. From the results presented in this paper we conclude that Gel-test is specific, clear, easy to perform and more sensitive than the traditional methods. It can be used for red cell phenotyping and antibody detection as well. By this technique it was possible to establish the specificity of antibodies of Rh system not found by any other method. The advantage of this test is that nearly all unspecific reactions could be eliminated. The disadvantage concerned the high sensitivity in the detection of cold, clinically insignificant allo- and autoantibodies. Therefore, some modifications for the elimination of these unfavourable reactions were introduced.

Adult↗
PubMed1992

Haemolytic disease of the newborn due to anti-Dia and incidence of the Dia antigen in Poland.

A strong complement-fixing incomplete anti-Dia was found in the serum of a Polish mother who gave birth to a newborn suffering from a severe haemolytic anaemia. The whole family was of Polish origin. 9,661 donor blood samples from different regions of Poland were tested against antiserum derived from that mother. Dia antigen was present on red cells of 45 (0.46%) individuals. All of them were of Polish origin. In some cases, family studies were undertaken.

Adult↗
PubMed1991

[Value of using polyethylene glycol in the indirect antiglobulin test with erythrocytes].

A series of comparative investigations was carried out for establishing the value of the indirect antiglobulin test (IAT) in its first phase. The results showed that PEG, in relation to NaCl solution of low ionic strength, increased the reactivity of various antibodies of different specificity present in 72 studied sera. A significant superiority of IAT-PEG to IAT-BISS was noted in 45.8% of these sera. In search for antibodies in sera of 1160 patients IAT-PEG detected 7 antibodies not reacting in the IAT-LISS, and 5 of them had Rh system specificity, one was anti-K and one anti-Jka. Although the IAT-PEG was more sensitive than IAT-LISS in the detection of anti-Rh antibodies, it was less sensitive in this range than the enzymatic LEN test with which not only 5 of these antibodies were demonstrated but which detected also 8 antibodies not demonstrated in IAT-PEG or IAT-LISS. All 5 antibodies not detected by the IAT-PEG but demonstrated in the IAT-LISS were specific for the Lewis system. The per cent of reactions regarded as non-specific in the IAT-PEG was 1.3%. The IAT-PEG modification may be recommended as a sensitive, simple and specific method extending the possibilities of immunohematological diagnosis, especially in cases of post-transfusion reactions and in difficulties of interpretation connected with the occurrence of weakly positive reactions in the IAT-LISS.

Blood Group Antigens↗
PubMed1991

[Studies of occurrence of antigen Dia from the Diego blood-group system in the Polish population].

In 9661 blood donors from seven regions of Poland studies were carried out for detection of the Dia antigen in erythrocytes. This antigen was found in 45 (0.46%) subjects not consanguinous. The prevalence of Dia in various regions of the country varied from 0.25 to 0.91%. Family studies in 23 cases with 94 subjects participating demonstrated inheritance of the antigen. It was tried to explain the presence of the Dia in the Polish population, since in the pertinent literature it is regarded as a characteristic trait of Mongoloid peoples.

Blood Donors↗
PubMedDate not supplied

Auto-anti-D blocking D determinants on red cells in pregnancy.

Positive direct antiglobulin test due to IgG1 autoantibody of anti-D specificity on red cells of an apparently healthy pregnant woman was found. At the period of the highest autoantibody activity D epitope were completely blocked and even by special methods used for Rh typing in patients with AIHA the detection of D antigen was not possible. Free auto-anti-D caused benign HDN in the infant. The autoantibody production was transient and stopped after delivery. D variant included into category V on the red cells of the patient under study and her two siblings was recognized. This finding, confirmed by in vitro study, gives the explanation of the complete blocking of D epitopes by anti-D autoantibody.

Adult↗
PubMedDate not supplied

The first example of anti-Dia antibody in Poland.

A strong incomplete and complement-fixing anti-Dia within Diego blood group system was found in the serum of a Polish mother who gave birth to a newborn suffering from a severe haemolytic disease. In family studies of three generations Dia antigen in three persons was detected and its hereditary character was confirmed. The whole family is of Polish origin.

Blood Group Antigens↗
PubMedDate not supplied

Alloantibody anti-Gerbich: blood transfusion problems and family studies.

In a patient with myelodysplastic syndrom complement fixing IgG1 anti-Ge three weeks after Ge positive serologically compatible blood transfusion was found. The patient and one of his children were Ge-1, -2, -3. Red cells were elliptocytic. The patient's nonconsanguineous wife and other family members were Ge positive. In the red cell membranes of Ge negative persons glycophorin C was lacking. Abnormal glycophorin was present in the red cell membranes of all family members. Anti-Ge appeared to be of no clinical significance. Transfusion of serologically incompatible Ge positive blood provided beneficial effect. After this transfusion anti-Ge disappeared from the serum and was not detected at the whole follow-up, although the patient received Ge positive blood several times. Immunological tolerance towards Ge antigen is suggested.

Blood Group Antigens↗
PubMedDate not supplied

[Anti-G antibodies from the RH system. I. Diagnostic antibodies and their clinical significance].

The complex diagnosis of anti-G antibodies in a subject with a not yet described phenotype Rh CcDEeG-is described. The presence of this phenotype made difficult finding of blood for transfusion. On the basis of the available literature the observations are discussed on anti-G antibodies and the views on the genetic determination of the G antigen on the erythrocytes in the light of the phenotypes described as yet.

Aged↗
PubMedDate not supplied

[Value of the LEN (LISS-enzyme) technic in detection of alloantibodies to erythrocytes].

The reported study was done for assessment of the value of the enzymatic technique LSE/LISS Spin Enzyme proposed by Odell et al. using a solution of NaCl of low ionic strength (LISS) for detection of incomplete alloantibodies to erythrocytes, especially those from the Rh system. The results of the study demonstrated that the LSE test, called by the authors LEN (LISS-Enzyme) is a sensitive and specific method comparable in its effectiveness to the two-step papain test (2-Pap) used widely in this country. In routine studies the LEN technique is simple, easy and rapid. Washing of papain treated erythrocytes is not necessary. The time from enzyme addition to erythrocytes to result reading is about 15 minutes, that is it is four times shorter than the time in the 2-Pap technique (60 minutes). A significant was the preparation of one type of erythrocyte suspension in LISS solution for two tests: enzymatic and antiglobulin, carried out in parallel. For these reasons the LEN test may be recommended in place of 2-Pap for detection and identification of antibodies. It is also a practical methods for matching test in place of the one-step papain test. The LEN test should be widely introduced in laboratories of transfusiological immunology in this country. Attention is called ti various technical details necessary for obtaining of unequivocal and reproducible results.

Erythrocytes↗
PubMedDate not supplied

[Anti-M antibodies in the pathogenesis of hemolytic disease of the newborn].

Two cases are presented of serological fetomaternal incompatibility in the MNSs group system diagnosed for the first time in Poland. Anti-M antibodies of the mother caused in one newborn haemolytic disease with acute anaemia requiring blood transfusions. In the other newborn clinical signs of the disease failed to appear despite a positive direct antiglobulin test. The results are presented of immunohematological studies calling attention to difficulties connected the diagnosis of haemolytic disease of the newborn caused by anti-M antibodies. This problem is discussed more extensively in the light of available literature.

Erythroblastosis, Fetal↗