PubMed Health⌕ Search

Biomedical subjects

G Kudesia

Publications and source records attributed to G Kudesia.

At least 19 recordsLinked to original sources
PubMed1992

Serological cross-reaction between Legionella pneumophila and campylobacter in the indirect fluorescent antibody test.

Sera from 50 patients with culture-proven campylobacter gastroenteritis were examined for the presence of antibodies to Legionella pneumophila. Ten patients (20%) had a positive titre (> or = 16) as measured by indirect immunofluorescence. Antibodies were detected in only 1 of 36 acute sera but in 10 of 14 (71%) sera obtained more than 10 days after the onset of symptoms. All positive sera contained specific IgM antibodies but specific IgG or IgA could not be detected in any sample. No legionella antibodies could be detected in sera from 42 similar patients with salmonella gastroenteritis. These results were shown to be due to serological cross-reaction between L. pneumophila and campylobacter.

Antibodies, Bacterial↗
PubMed1992

Diagnosis of cytomegalovirus infection in heart transplant recipients.

In a prospective study eight (47%) heart transplant recipients had recurrent cytomegalovirus (CMV) infection. Of these, five had received hearts from seropositive donors. Neither serology nor virus isolation from urine was reliably associated with clinical CMV infection, but a high CMV IgM index correlated with CMV infection.

Antibodies, Viral↗
PubMed1991

Clinical rubella reinfection--a case of arthropathy.

We describe a case of arthropathy due to rubella reinfection. Clinical rubella reinfection with rash and lymphadenopathy has been reported but as far as we are aware this is the first report of a case with arthropathy as the sole presentation. Rubella IgG subclass and avidity tests confirmed the reinfection.

Adult↗
PubMed1991

Comparison of ultracentrifugation and polyethylene glycol precipitation for concentration of hepatitis B virus (HBV) DNA for molecular hybridisation tests and the relationship of HBV-DNA to HBe antigen and anti-HBe status.

A 32P-labelled DNA probe was used to examine 50 hepatitis B surface antigen (HBsAg)-positive sera for the presence of hepatitis B virus (HBV) DNA. HBV-DNA was detected in all 21 HBeAg-positive samples, in one out of 21 anti-HBe-positive samples and in three out of eight HBeAg- and anti-HBe-negative samples. The results of this DNA hybridisation test correlated well with HBeAg status and could be used to determine infectivity in HBeAg- and anti-HBe-negative samples. Ultracentrifugation was marginally superior to polyethylene glycol precipitation for concentrating HBV-DNA from serum.

Chemical Precipitation↗
PubMed1991

Comparison of cell culture with an amplified enzyme immunoassay for diagnosing genital herpes simplex infection.

An amplified enzyme immunoassay (EIA) for herpes simplex virus (Novo Nordisk) was compared with cell culture in 853 genital specimens from a genito-urinary medicine clinic. The sensitivity of the EIA was 86% and its specificity 99.6%. The sensitivity increased to 94% for lesion swabs but decreased to 68% for cervical swabs. Sensitivity for urethral and vulval swabs was 83% and 82%, respectively. It is concluded that the EIA is specific and quick and easy to perform. It will be suitable for testing for genital herpes simplex infections in laboratories without access to local cell culture facilities.

Cells, Cultured↗
PubMed1990

Enzyme linked immunosorbent assay for detecting adenoviruses in stool specimens: comparison with electron microscopy and isolation.

A commercial enzyme linked immunosorbent assay (ELISA) for detection of adenoviruses in stool samples was compared with the use of electron microscopy and isolation in Graham 293 cells. Although specific, the ELISA was less sensitive than both electron microscopy and isolation. The ELISA had an overall sensitivity of 78% and a specificity of 100%. The sensitivity was related to the amount of virus particles present in the stool sample, increasing to 90% with about 10(7) viral particles/ml of stool. The ELISA was easy to perform, requiring no instrumentation, and is a useful first line test for detection of adenoviruses in stool samples, especially in laboratories without access to an electron microscope. Wider use of ELISAs should help in evaluating the role of adenoviruses in viral gastroenteritis.

Adenoviruses, Human↗
PubMed1990

Incidence of herpes simplex virus type-1 and type-2 from patients with primary (first-attack) genital herpes in Sheffield.

In a study to determine the herpes simplex virus (HSV) type isolated from patients with primary (first-attack) genital herpes and to identify any clinical or epidemiological features that might influence the reported incidence of any particular viral type, there was almost an equal incidence of HSV-1 and HSV-2 isolated from women (48% versus 52%). In the case of women, the initial clinical diagnosis was correct in 97% with HSV-1 and 95% with HSV-2 infection. Amongst men, HSV-2 was isolated more frequently than HSV-1 (71% versus 29%). However, the initial clinical diagnosis in men was correct in only 53% with HSV-1 and 83% with HSV-2 infection. These results suggest that in primary genital herpes the incidence of HSV-1 and HSV-2 is probably equal, while in men the reported incidence of HSV-1 may be affected by underdiagnosis giving a falsely high incidence of HSV-2 infection.

Adult↗
PubMed1988

Hepatitis A in Scotland--is it a continuing problem?

The prevalence of anti-HAV in blood donors in West Scotland and in children admitted to a Glasgow hospital was determined. By the age of five years, 16% of the children had antibody and at 15 years, 30%. In adults, prevalence continued to rise with age, with 78% of those over 40 years showing evidence of past infection. Cases of acute hepatitis A in West Scotland have declined since 1983. At present the peak age group for infection is 25-34 years whereas in the early 80s the peak group was five to 14 years.

Acute Disease↗