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Biomedical subjects

G L Hart

Publications and source records attributed to G L Hart.

7 recordsLinked to original sources
PubMed1994

Conformation polymorphisms and targeted marker development.

Genetic markers were developed for three targeted bovine loci using both single-stranded and double-stranded DNA conformation polymorphisms. Eight of nine DNA fragments exhibited single-stranded conformation polymorphisms while only one of nine exhibited a double-strand conformation polymorphism. All but one of the polymorphic fragments exhibited two allelic forms, with the exception being a single-stranded conformation polymorphism with three alleles. Utility of conformation polymorphisms relative to microsatellite markers for linkage map development and quantitative trait loci (QTL) mapping was assessed by comparing frequency of heterozygote parents from reference and resource families. Heterozygosity was greater for microsatellite markers (P < 0.01) and reference family parents (P < 0.01), though the disparity between marker types tended to be less dramatic for the reference family parents (P < 0.09). These results suggest conformation polymorphisms will be a useful tool for targeted marker development in linkage map and comparative map development provided genetically diverse families are studied.

Animals↗
PubMed1985

Standards for the assessment of visual evoked potentials in an ethnically heterogeneous adult population.

Visual evoked potentials (VEPs) were recorded by chequerboard pattern-reversal stimulation in 276 normal adult subjects aged 15-73 years. The sample comprised comparable white, coloured and black groups of both sexes. Significantly shorter latencies of the major positive component were found in both male and female black subjects compared with those in whites. No significant differences were found between the amplitudes in the different population groups, but females in each population group showed significantly higher amplitudes of the major positive component than males. There was a close relationship between latency and amplitude for both right and left eyes. Except in coloured females, a non-linear relationship of the major positive component with age was demonstrated, the shortest latency being at about 35 years of age. It is suggested that the following criteria be used in the evaluation of VEP recordings: latency as well as the difference between latencies and amplitudes of the two sides of the major positive component (P1) as compared with standardized values for ethnic, sex and age groups.

Adolescent↗