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Biomedical subjects

G L Powell

Publications and source records attributed to G L Powell.

At least 91 records · Page 5Linked to original sources
PubMed1981

Inservice training for part-time dental faculty.

Part-time faculty outnumber full-time faculty in clinical dentistry departments. They spend many hours in contact with students and often serve as role models of dental practitioners. Yet, part-time faculty have had very little formal training in instructional techniques. This article describes an inservice training program for part-time faculty involving workshops, weekly meetings, and practice sessions. The program was developed to provide training in some basic teaching techniques. Part-time faculty were given an instructional manual and copies of student syllabi and the opportunity to participate in the development and use of the student criteria sheets. The results of the program indicate that the faculty felt the program was an effective use of their time and they will accept, and even look forward to, future inservice training opportunities.

Educational Measurement↗
PubMed1980

Retention of part-time faculty.

Part-time faculty members are a large and important part of teaching programs at dental schools across the nation. Retaining part-time faculty members is important in order to provide consistency of instruction and because training new faculty requires time and effort that otherwise would go into building a stronger teaching program. This paper discusses methods used at the University of Washington School of Dentistry department of restorative dentistry to keep the part-time faculty actively engaged in dental education year after year. These methods include involving the faculty members in the entire teaching program, providing them with inservice training, and doing some personal things for them. The methods have proved successful as shown by the retention rate in the last three years.

Education, Dental↗
PubMed1980

Dental faculty training in clinical instruction.

Most dental faculty have had no formal training in education prior to their clinic instructor role and many report a need for training in educational methods and tutorial skills. Organized methods for establishing student performance criteria and improving instructional skills presented in a well-designed inservice training program utilizing prepared training materials, workshop learning, and practice sessions can increase teacher effectiveness in clinic instruction.

Education, Continuing↗
PubMed1978

Cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis.

The spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. The stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) of Trimeresurus flavoviridis. The results of treatment of the spin-labeled cardiolipin with the cardiolipin-specific phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) (Hemophilus parainfluenzae) of known specificity and with phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) of Bacillus cereus are consistent with the assigned structure. The spin-labeled cardiolipin is further characterized and the unique features of this diastereomer are discussed in the context of the unusual stereochemistry of the natural phospholipid.

Cardiolipins↗
PubMed1976

Acyl carrier protein metabolism and regulation of fatty acid biosynthesis by Lactobacillus plantarum.

Endogenous fatty acid biosynthesis in the bacterium Lactobacillus plantarum is greatly decreased upon addition of exogenous fatty acids (Henderson, T.A., and McNeil, J.J. (1966) Biochem. Biophys. Res. Commun. 25, 662-669). We have demonstrated the presence of five pantothenate-containing compounds in L. plantarum which have been identified by co-chromatography with authentic samples: pantothenate, 4'-phosphopantetheine, 3'-dephosphocoenzyme A, coenzyme A, and acyl carrier protein (ACP). The concentrations of the above pantothenate-containing compounds were found to be: 0.009, 0.13, 0.067, 0.69, and 0.22 nmol/mg of protein, respectively. L. plantarum ACP was shown to have a molecular weight near that of Escherichia coli ACP but to have a lower isoelectric point (pI = 3.75). Oleate in the presence of Triton X-100 was found to reduce the concentration of ACP by 80% with little effect on the concentrations of the other pantothenate-containing compounds. The synthesis of ACP apparently ceased soon after the addition of oleate and the rate of decrease in concentration of ACP was quanitatively consistent with the previously observed rate of decrease in the initial rate of fatty acid biosynthesis in this organism (Weeks, G., and Wakil, S.J. (1970) J. Biol. Chem. 245, 1913-1921). Thus, the change in rate of fatty acid biosynthesis in L. plantarum upon addition of oleate to the medium can be quantitatively related to the concentration of ACP (and probably to the concentration of co-repressible enzymes of fatty acid biosynthesis).

Biological Transport, Active↗
PubMed1975

Inhibition of citrate synthase by oleoyl-CoA: a regulatory phenomenon.

Fatty acyl-CoAs are good detergents (dritical micelle concentrations = 3-4 muM) and can inhibit a number of enzymes, including some involved in fatty acid biosynthesis. The regulatory significance of fatty acyl-CoAs as negative effectors has been questioned largely because of the difficulties in distinguishing possible nonspecific detergent effects from more specific regulatory interactions with these enzymes. A new analogue of oleoyl-CoA, oleoyl-(1, N6-etheno)-CoA, is a better detergent (critical micelle concentration = 3.2 muM) than oleoyl-CoA (critical micelle concentration = 4.7 muM). This new analogue is not as good (by an order of magnitude) an inhibitor of citrate synthase [citrate oxaloacetatelyase (pro-3S-CH2-COO-vectoracetyl-CoA); EC 4.1.3.7] nor is it bound as well oleoyl-CoA. Since the only difference between these two compounds is substitution of 1,N6-ethenoadenine for the adenine of CoA, the difference in inhibition and binding implies a specific interaction between the adenine moiety of oleoyl-CoA and citrate synthase. Moreover, since oleoyl-(1,N6-etheno)CoA is a better detergent than oleoyl-CoA, the detergency of oleoyl-CoA is not the sole cause of the fatty acyl-CoA inhibition of citrate synthase. These results support a physiological role for oleoyl-CoA as a negative effector for citrate synthase. An analogous physiological role for fatty acyl-CoA as negative effectors for other enzymes seems reasonable.

Animals↗