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G L Stoffels

Publications and source records attributed to G L Stoffels.

11 recordsLinked to original sources
PubMed1981

Nuclear size and nuclear binding of tritiated actinomycin D into epithelial cells of colon cancer patients with apparently normal colorectal mucosa.

The nuclear binding of tritiated actinomycin ([3H]AM) is clearly linked to the structural organization of the chromatin. [3H]AM decreases in cells which differentiate and becomes very low in fully differentiated cells. [3H]AM nuclear binding and nuclear size were concomitantly measured in normal-appearing flat mucosa of 10 patients bearing a colorectal cancer and compared with the colon mucosa of 10 normal individuals. In the colon of these normal subjects, there is a decreasing gradient of labeling in the upper third of the gland, with heavy labeling in the lower two-thirds and light labeling in surface epithelial cells. The ratio (R) of grain count in cells in the bottom of the glands to grain count in the surface epithelial cell varies in normal subjects from 4 to 14. There is no correlation between nuclear size and [3H]AM binding. In normal-appearing flat mucosa of cancerous patients, there is no decrease of labeling [3H]AM in the upper third of the glands; superficial cells are as labeled as those at the bottom (R = 1). There is a correlation between nuclear size and [3H]AM in these cells. Normal-appearing cells of colorectal cancer patients are probably not involved in a normal process of differentiation.

Autoradiography↗
PubMed1979

Cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.

The nuclear binding of H3 actinomycin, which is closely linked to the differentiation phenomenon, was studied in human normal gastric mucosa. Actinomycin binding decreases in cells which differentiate and becomes very low in fully differentiated cells. In the gastric pits, there is a decreasing gradient of labelling from the deeper stem cells to the well-differentiated superficial cells. This indicates that migration and renewal of the surface epithelium occurs following a 'pipe-line' system. All the undifferentiated stem cells are labelled. Where the parietal cells are concerned all degrees of labelling are observed at various levels with a decreasing proportion of labelling from the surface to the bottom of the gland. With mucous cells and chief cells in the upper part of the gland a great number of poorly labelled mucous neck cells is observed. In the middle and lower part of the gland there is a new growth of heavily labelled cells. This means that in the normal human stomach chief cells probably do not originate from mucous cells.

Adult↗
PubMed1978

Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

Triated actinomycin binding to DNA is closely linked to the degree of repression in chromatin. 3H-AM binding to DNA is the most pronounced in nuclei of cells committed into cycle. Inversely, in cells in the last steps of their differentiation or (and) in the resting state (non-dividing cells), 3H-AM binding for DNA is diminished down to a baseline since it is limited by the deoxynucleoproteins. Epithelial cells of stomach mucosa and duodenum demonstrate an increased cell uptake of tritiated actinomycin from the surface to the bottom of the pits. In severe gastritis and in intestinalysed metaplasia this was abolished: with a uniform enhancement of 3H-AM binding. These findings seem to indicate that these cells are derepressed.

Atrophy↗