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Biomedical subjects

G Lischka

Publications and source records attributed to G Lischka.

At least 19 recordsLinked to original sources
PubMed1988

[Anaphylactic reaction to human insulin. Successful desensitization].

A 48-year-old woman with diabetes mellitus (type IIb) had to be treated with insulin because of poor blood-sugar control. Infiltrates at the sight of injection occurred with two different porcine insulin preparations, necessitating a switch to human insulin. After the third injection of the latter an anaphylactic reaction developed within minutes. During a pregnancy in 1968 the patient had briefly received bovine insulin. Tests now revealed an immediate-type allergy against all three available species insulins, which was successfully treated by hyposensitisation.

Anaphylaxis↗
PubMed1979

Lymphocyte proliferation during PUVA therapy.

The lymphocyte proliferation of 15 psoriatic patients was sutdied after stimulation with tuberculin, trichophytin, varidase, concanavalin A (Con A), and pokeweek mitogen (PMW) during the first and the eighth PUVA treatment, 2 h after oral intake of 8-methoxypsoralen (8-MOP), immediately after UVA-irradiation, and 24 h later. There was a significant decrease of the lymphocyte proliferation, after stimulation with Con A (P less than 0.05), trichophytin (P less than 0.01) and varidase (P less than 0.05), not with PWM and tuberculin, during the first PUVA treatment by 8-MOP alone. This decrease was not significantly changed by the following UVA irradiation and could not be demonstrated after 24 h. No dark effect of 8-MOP on the lymphocyte proliferation could be seen during the eighth treatment. 8-MOP seems to influence lymphocyte proliferation in the dark by interfering with the cell surface, not with the nucleus. The disappearence of this effect during PUVA therapy could be explained by a modification on the surface structure of lymphocytes.

Adolescent↗
PubMed1978

Dark effect of 8-methoxypsoralen (8-MOP) on lymphocytes.

In order to find, whether 8-MOP has an inhibiting effect on the proliferation of lymphocytes in the dark, we studied in vitro the lymphocyte proliferation by a mitogen (HgCl2) and by an antigen (tuberculin) from 16 psoriatic patients during the first oral PUVA treatment immediately before 8-MOP intake (03.--0.5 mg/kg body weight), 2 h later (time of maximal serum concentration) and after 24 h. The percentages of B- and T-lymphocytes were measured from 15 other psoriatic patients at equal times. There was a significant decrease (P less than 0.05) of the lymphocyte proliferation after stimulation with HgCl2 in culture, 2 h after 8-MOP, returning completely to the starting range after 24 h. On the contrary, neither the proliferation capacity of lymphocytes after stimulation with tuberculin nor the percentages of B- and T-lymphocytes were significantly changed. Obviously, 8-MOP, even without UVA irradiation, has a direct inhibiting effect on lymphocyte proliferation in concentrations therapeutically used. However, this effect is seen only with stimulation by a mitogen, not by an antigen. It cannot be explained by a change in the ratio between B- and T-lymphocytes.

Cell Count↗
PubMed1977

[Effects of UV-C and of 8-MOP + UV-A on the T- and B-population of human lymphocytes in vitro (author's transl)].

The transformation of UV-C irradiated leucocytes and lymphocytes by the mitogens Con A, PHA and PWM is measured by the 3H-Tdr. incorporation after 72 h incubation. Furthermore T- and B-cells are determined by the method of rosette formation. A clear inhibition of the cell activity is seen after irradiation of leucocytes with 450 mJ/cm(2) and of a lymphocyte suspension with 5-10 mJ/cm(2). There are no significant differences between the effects of the various mitogens. The number of T-cells decreases proportionality to the various intensity, the number of B-cells remain constant. Irradiation with UV-A + 8-MOP cause, equally for all mitogens, a dosis dependent inhibition of thymidine incorporation.

B-Lymphocytes↗
PubMed1977

Effects of 8-methoxypsoralen (8-MOP) and UVA on human lymphocytes.

Peripheral lymphocytes of 37 psoriatic patients are tested before and under PUVA treatment using as parameter the non specific stimulation effect of HgCl2 (10 microgram/ml) in culture, measuring the 3H-thymidine incorporation after the last 16 h of a 5-days culture. Oral 8-MOP in therapeutic doses is decreasing the lymphocyte stimulation as well as 8-MOP together with UVA irradiation during the first week of treatment. After 1 week, the stimulation is, on the contrary, significantly enhanced after irradiation. Lymphocytes isolated by centrifugation over Lymphoprep are submitted to PUVA conditions in petri dishes (Hank's solution 8-MOP 1 microgram/ml, irradiation with 350 nm, 93-372 mJ/cm2). The total cell number, the E-rosette formation (as marker for T-lymphocytes) and the EAC-rosette formation (as marker for B-lymphocytes) are determined. PUVA conditions have an energy dependent decreasing effect on the cell number, while the T- and B-cell proportions remain constant. UVA irradiation alone has such an effect only with high energies. 8-MOP without UVA has no significant influence on the cell number.

Humans↗
PubMed1977

[Pityrosporum orbiculare in acneiform eruptions].

Six patients with acneiform eruptions in the predilection sites of pityriasis versicolor are briefly described. Histologically there were abscesses below the acroinfundibulum wherein yeasts could be found with the characteristics of pityrosporum orbiculare. The possible pathogenity of these fungi is discussed.

Acne Vulgaris↗
PubMed1976

[Cutaneous granulomatous cladosporiosis].

The case of a 41 year old man with a tumorlike cutaneous cladosporiosis on the fifth right finger is described. Histologically seen there was an unspecific granulomatous inflammatory cellular infiltrate in the upper and middle corium, whereas in the deeper corium there were found big macrophages phagocytizing fungical elements pointing to cladosporiosis. The fungi grew up very slowly at a temperature of 30 degrees C. Since 1971 the patient was suffering from a "symptomatic" epilepsy medicamentally controlled. Though a neurotropic action of some cladosporion species is well known there could not be found any hint for a cerebral abscess.

Adult↗
PubMed1976

[Lymphokine activity in mercury stimulated leucocyte cultures (author's transl)].

Blood-lymphocytes from 42 persons reacted, as expected, in the leucocyte-culture with an increased DNA-synthesis, measured by the uptake of tritiated thymidine during the last 16 h of a five days culture, if mercurybichloride was added at the onset of culture. The logarithms of the activity-indices were nearly symmetrically distributed. When the lymphokine-activity in the culture supernatants was studied with the indirect leucocyte-migration-test, using autologues leucocytes, there was a bimodal distribution of the logarithmic migrationindices with a high top in the negative area (migration-inhibition) and a lower one in the positive area (migration-stimulation). These phenomena had no detectable correlations to sex, age, or dermatosis of the patients.

Cells, Cultured↗
PubMed1975

[Mitogenic activity of melanoma extracts in leucocyte cultures (author's transl)].

From a mixed cell suspension of five primary melanomas an aqueous sterile extract was made and added to homologue leucocyte cultures of eight melanoma patients and seven controls. The extract had a blastogenic and mitogenic effect in all cultures. There was no significant difference between the responses of patients and controls. In experiments with an equally prepared extract of normal skin, added to homologue leucocyte cultures of nine persons, none but one had a positive reaction. This indicates, that the motigenic principle of the melanoma extract is not related to the histocompatibility antigen nor caused by chemical or bacterial contamination of the primary cell suspensions. It seems to be derived from the cytoplasmic fraction of melanoma cells. Other experiments to solve the problem were impossible because of lack of material. There are several reports in the literature which by using different methods indicate, that there might be an immunological reaction to melanoma associated principles in healthy persons, too.

Cell Transformation, Neoplastic↗