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Biomedical subjects

G M Butenko

Publications and source records attributed to G M Butenko.

At least 19 recordsLinked to original sources
PubMed2004

Effect of epithalamin on circadian relationship between the endocrine function of the thymus and melatonin-producing function of the pineal gland in elderly people.

The effect of epithalamin on circadian rhythms of thymic serum factor titers and melatonin concentrations in the blood of elderly people was studied. Course treatment with epithalamin modulated the rhythm of the thymic endocrine function. The increase in the titer of thymic serum factor at night coincided with the increase in blood melatonin concentration and shift of its acrophase to 3.00, which was characteristic of young people. In elderly people with preserved nocturnal peak of the thymic serum factor titer before therapy the nocturnal (3.00) concentration of melatonin was high and did not change after drug therapy. The correlation between melatonin concentration and titer of thymic serum factor increased after epithalamin treatment.

Aged↗
PubMed2004

Effects of bioactive factors of the pineal gland on thymus function and cell composition of the bone marrow and spleen in mice of different age.

The effects of factors from the pineal gland on the titer of thymic serum factor in the supernatant of 3-h thymus stroma cultures, number of stromal precursor fibroblasts and CD4+ cells in the bone marrow, and CD8+ cells in the spleens of adult and old CBA mice were studied in vitro. Epithalamin, Epithalon, and melatonin appreciably increased the titer of thymic serum factor in the supernatant of thymus stroma cultures from mice of different age and increased the percentage of CD4+ cells in the bone marrow suspension from old animals in vitro. The percentage of CD8+ lymphocytes decreased after incubation of splenic cells from old mice with melatonin. The percentage of bone marrow fibroblast precursor cells from adult and old mice did not appreciably change after incubation with the preparations.

Animals↗
PubMed2004

[The pineal gland's peptides factors and the rhythms of functions of the thymus and bone marrow in animals during aging].

It was investigated the influence of pineal peptides on the aging changes both circadian rhythm of thymic serum factor (FTS) titer in male rats Wistar and circannual rhythms of FTS, the amount of stromal cells-precurcors (CFC-F), granulocyte-macrophage cells-precurcors (CFC-GM), CD4+, Mac-1+ and CD19+-cells in bone marrow of male mice CBA. Epithalamin was injected chronically beginning from 6 month in rats and 4 month in mice. In old animals the rhythmical disturbancers of the indices characterized by loss of fluctuations, displacement of seasonal acrophase, increased or diminished amplitude, inversion of rhythm and desynchronization. After epithalamin injections in rats of 11, 17, 27 months the FTS titer at night increased and in old mice of 23-24 months appeared the peak of hormone level in summer-autumn, restored the difference between the amount of CD4+, Mac-1+-cells in bone marrow in spring and autumn, increased CFC-GM amount in spring, diminished CFC-F in autumn and increased this indice in winter. Epithalon also diminished of CFC-GM and CFC-F amount in old mice in autumn. The retarding age-related disturbances of suprachiasmaticus nucleus of hypothalamus structure, the diminishing corticosterone and testosterone levels at night, the increasing hormones level in summer and the falling in winter plays role in the improvement of thymus and bone marrow rhythmical function in old animals, reseaved epithalamin. The rhythms of most indices rhythms in old animals showed a pattern of adult.

Aging↗
PubMed2003

[Regulating effect of pineal gland peptides on development of T-lymphocytes in CBA aging mice: role of microenvironment of immune system organs and neuroendocrine factors].

Studies were undertaken on the development of T-lymphocytes in adult and old CBA mice and its changes at aging after injections of pineal gland peptides. It was shown that in old mice the disturbances of T-cells differentiation are registered in bone marrow, thymus, spleen and characterized by the changes of lymphocyte markers expression, migration and proliferation of cells. In old mice FTS titer, melatonin and testosterone levels decreased, the balance of noradrenalin and serotonin in hypothalamus and the cell composition of microenvironment immune systems organs impaired. After chronic (18 mo) administration of the pineal gland preparation epithalamin the amount of stromal cells-precursors, CD4+ and Mac-1(+)-cells in old bone marrow increased, improved the migration of T-cell precursors from bone marrow to thymus and their proliferative potential. The proportion of CD3+, CD4+CD8-, CD4-CD8+, Mac-1(+)-cells in old thymus increased, while that of CD44(+)-cells decreased. The proportion of CD4-CD8(+)-cells in spleen increased. The most number of indices and their balance showed a pattern of adult mice. In old mice after epithalamin the balance of amines in hypothalamus improved, concentration of melatonin in pineal gland, testosterone and FTS titer in blood increased. Epithalon has also the possibility to increase of thymic endocrine function.

Aging↗
PubMed1992

[Effect of antihypertensive therapy with captopril on gluco- and mineralocorticoid receptors of peripheral blood lymphocytes in hypertensive patients of various age].

Binding of 3H-dexamethasone and 3H-aldosterone by peripheral lymphocyte receptors was investigated in healthy persons and hypertensive patients before and after 2-week captopril treatment. The number of glucocorticoid and mineralocorticoid binding sites was increased in hypertensives vs normotensives. The treatment with the ACE inhibitor captopril led to activation of hormone-receptor interactions. There was a more marked rise of the number of receptors in middle-aged (44-55 years) hypertensives vs elderly (61-80 years) subjects after captopril treatment.

Adult↗
PubMed1990

Stromal hemopoietic microenvironment in aging.

The bone marrow content, proliferative potential and proliferative activity of precursor cells for stromal fibroblast colony forming cells (CFC-F) were investigated in young and old CBA mice. The relationship between CFC-F and the number of bone marrow nucleated cells and granulocytic-macrophagal precursors (GM-CFC) was studied as well. The results obtained showed increased CFC-F contents in old animals. The proliferative potential of old mice CFC-F did not appear to differ from that of young animals. The proliferative activity of bone marrow CFC-F and hemopoietic stem cells--spleen colony forming cells (CFC-S) was studied by determining the sensitivity to hydroxyurea administration. The responses were almost the same in young and old mice. A direct correlation between CFC-F and nucleated cells and GM-CFC precursors was found in young mice, but not in the old animals. The results of the present study have pointed to the reorganisation of the stromal tissue microenvironment in bone marrow in old age.

Aging↗
PubMed1990

Increased glycation and pigmentation of collagen in aged and young parabiotic rats and mice.

Changes of non-enzymatic collagen glycosylation were followed in 2- and 24-month-old rats and mice and in parabiotic animals of the same age. With advancing age increased glycation of collagen was observed in both old male Wistar rats and white mice. Further it was demonstrated that both aortal and skin collagen of young animals is rapidly non-enzymatically glycosylated in the common milieu created by parabiotic animals and the proportion of non-enzymatically incorporated glucose approaches in the young counterparts the level found in old individuals. Similar trends as with non-enzymatic glycosylation were found with a fluorescent (370/440 nm) product present in both categories of collagen preparations. This fluorescence was higher in old animals and was considerably increased in the young counterpart of the parabiotic couple 6 weeks after operation. The nature of the fluorescent product appears different from pyridinoline and remains to be elucidated.

Aging↗
PubMed1990

[Active mechanisms of dysfunction in the process of aging].

Current views on the causes and mechanisms of aging are discussed. Evidence for active mechanisms which are regulatory in their origin and operate at the body and its systemic levels is presented. These mechanisms are believed to be capable of inducing changes in the organism thus bringing its functioning to the level which is characteristic of old age. Such phenomena have been observed in the immune and reproductive systems, connective tissue, skin, and liver. Examination of heterochronic parabionts revealed that these mechanisms affect the general viability of a young organism paired to an old one causing a marked reduction of the young parabiont's life-span up to the life limit of his old partner. This suggests a possible role played by aging in a species' adaptation to the environmental conditions.

Adaptation, Physiological↗
PubMed1990

[Effects of the age of the recipient on the outcome of transplantation of lymphoid organs from newborn donors].

The comparison of developmental capacities of simultaneously grafted thymus and spleen taken from newborn CBA mice in young and old macroenvironment was attempted using the method of heterotopic chimera construction. The perceptible augmentation of immune response in both host and donor spleens was obtained in case of transplantation into the young animals, and completely opposite effect, i. e. well-marked immunosuppression in case of old recipients. Preliminary removal of host spleen results in abolition of this suppressive effect in old animals. Moreover, immune response in donor spleen following thymus transplantation into the old splenectomized recipients was significantly higher compared to the same one without additional thymus graft. Taken together, these findings indicate that despite the existence of some potency for autonomous development the eventual effect of neonatal lymphoid organs maturation strongly depends upon the age of system in which this maturation does take place.

Age Factors↗
PubMed1987

[Cessation of ovarian cycles in a young mouse while in parabiosis with an older one].

Female mice 3 and 22-24 months of age were jointed in heterochronic parabiosis. That led to permanent estrus in young mice and maintenance of intact anestrus in old mice. After 8-9 weeks of parabiosis there appeared morphological signs of accelerated ageing in the ovaries of young animals, while no changes in the ovaries of old mice were observed. Serum progesterone of young partners decreased to the levels of old animals and estradiol levels remained unchanged, while prolactin content in adenohypophysis was the same as in young single animals but exceeded the level noted in young mice in parabiosis with young partners. Hormone content in old parabionts remained unchanged, as compared to the control. An old organism is thought to be the source of unidentified factors suppressing the ovarian function in young animals.

Aging↗
PubMed1986

Functional rearrangement of lymphohemopoietic system in heterochronically parabiosed mice.

This study examines some characteristics of the lymphohaemopoietic system of heterochronically parabiosed CBA mice. It was found that a decline of the primary immune response in a young mouse sutured with an old one is accompanied with the diminution of: bone marrow and thymus cellularity; thymus weight; haemopoietic stem cells (CFC-S) and granulocyte-macrophage precursor cells (CFC-C) contents in the femur, and stromal precursor cell (CFC-F) contents both in the femur and thymus. All the indices tested, including the immune response level, in old parabionts vs. old single mice remained unchanged. In the spleen, the considerable variations of splenic weights in the mice of different control and experimental groups have been found. At the same time, no significant changes in the cellularity and CFC-F number in the spleen of all examined groups of animals have been observed. The findings strongly indicate the considerable rearrangement of lymphohaemopoietic and stromal tissues in the central organs of the immune system, namely, bone marrow and thymus of young mice due to the parabiosis with old ones.

Aging↗
PubMed1985

Effect of thymus grafts of various ages on the immune system formation in CBA mice.

An attempt has been made to govern the development of immunological capability in CBA mice by means of transplantation at an early postnatal period of thymus from donors of various ages. The results have shown that in thymectomized animals, apart from a well known gradual decline with the donor's age in restorative functions of the transplanted thymus, there was at the age of 22 months a phase of inhibitory influence on the recipient's immune system. In intact animals, transplantation of the thymus from a 5-day-old donor caused a decrease and that from a 22-month-old donor caused an increase in the development of the capacity for immune response. Taken together, our findings indicate an active role of the thymus both in the formation of the immune system and in its alteration with aging.

Aging↗
PubMed1985

Ageing of the immune system and diseases.

Age-associated changes in immunity, which are manifested by a reduced immune response and a greater frequency of autoimmune events, are closely linked to the pathology of ageing. Further indications that this is so are the more marked changes in immunity indices in old subjects suffering from cardiovascular, nervous and bone and joint diseases; using other indices, however, such changes appear to be less pronounced. Ageing of the immune system is accompanied by suppression of cellular and humoral elements of older individuals, as demonstrated clearly by experiments involving heterochronic parabiosis and heterochronic transplantation of cells and organs (thymus, spleen and bone marrow). Our findings may suggest that changes in the immune system with ageing are not a consequence of loss or decreases in certain cells or substances; rather, they are due to active suppression of the regulation of cell differentiation and of cooperative interaction between various types of cells. Such effects may be of a systemic or local character and reflect the expression of the organism's ontogenetic programme. In turn, primary disturbances of the immune system can cause several of the secondary ageing phenomena, dominated by various forms of pathology.

Adult↗
PubMed1985

[Ultrastructure of human blood T-lymphocytes marked with monoclonal antibodies].

The ultrastructure of different human blood T-lymphocyte subpopulations was studied by immunoelectron microscopy using "Orthoclone" monoclonal antibodies (OKT-3, OKT-4 and OKT-8) and peroxidase-antiperoxidase (PAP) complex. It was shown that T-lymphocyte subpopulations, distinct in their surface markers and function, possessed some specific features of submicroscopic structure. OKT-4+ cells (helper/inductor) were characterized by small size, high nucleus/cytoplasm ratio and few cytoplasmic organelles. OKT-8+ cells (suppressor/killer) were larger, had low nucleus/cytoplasm ratio and bean-like nucleus, with their cytoplasm containing numerous mitochondria, well-developed Golgi complex and osmiophilic granules.

Antibodies, Monoclonal↗