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Biomedical subjects

G Manuel

Publications and source records attributed to G Manuel.

6 recordsLinked to original sources
PubMed1996

Quantification of phenylbutazone in equine sera by use of high-performance liquid chromatography with a nonevaporative extraction technique.

OBJECTIVE: To develop a sensitive, rugged high-performance liquid chromatography (HPLC) method for the measurement of phenylbutazone (PBZ) in equine sera, using a rapid, nonevaporative extraction technique. SAMPLE POPULATION: Sera from 5 nonexercising adult horses. PROCEDURE: After addition of sodium chloride and acetonitrile to serum samples, reverse-phase HPLC analysis for PBZ and oxyphenbutazone (OXY) was performed directly on extracts, using diode array UV spectrophotometric detection. Probenecid was used as an internal standard. Data were evaluated by standard means of statistical analysis. RESULTS: Recoveries of PBZ, OXY, and probenecid from spiked samples were acceptable (ie, > or = 80%) and within run retention times were reproducible. Chromatograms were free of interfering substances, and linearity of calibration curves was observed throughout operational ranges. Coefficients of variation at each fortified PBZ concentration were in the 5 to 10% range. The method was applicable to analysis of PBZ and OXY in serum extracts from horses dosed with PBZ (4.4 mg/kg of body weight, IV) in a controlled environment. Track samples analyzed by use of this method and a conventional liquid/liquid extraction method gave comparable results (mean deviation, 1.6%) for PBZ concentrations. CONCLUSION: The HPLC protocol described is suitable for measuring PBZ and OXY in equine sera to regulate PBZ administration in horses involved in pari-mutuel racing.

Animals↗
PubMed1982

Improved survival mediated by Corynebacterium parvum following allogeneic bone marrow transplantation in rabbits.

The effect of Corynebacterium parvum on survival was studied in aplastic rabbits transplanted with allogeneic bone marrow cells. Twenty four hours after receiving 1200 rad of total body irradiation, NZW males were reconstituted from unrelated R females using bone marrow combined with spleen cells or with marrow alone. The effects of treating donor, or both donor and recipient, with C parvum were contrasted. Rabbits in Group I were infused with a mixture of bone marrow and spleen cells. In control aminals (Group Ia) none of the 14 animals survived to 40 days. Similarly, there were no survivors among 17 animals in group Ib where only the donors were pretreated with C. parvum. When both donor and recipient received this agent (Group Ic) three of the 13 animals (23%) survived beyond 40 days. Animals in Group II were infused with marrow cells alone. Of the controls (Group IIa), 3 out of 24 animals (12.5%) survived to 40 days; pretreatment of the donor with C. parvum (Group IIb) resulted in survival of 8 of the 18 animals (44%); when both donor and recipient received this agent (Group IIc), 16 of 26 animals (62%) survived beyond 40 days. Both the incidence and the severity of histologically demonstrable graft-versus-host disease was markedly reduced in the surviving animals. These observations demonstrate a significant effect of parenteral C. parvum on the prolongation of survival in the irradiated rabbit following transplantation with allogeneic bone marrow cells, which is related to reduction in GVHD.

Anemia, Aplastic↗
PubMed1976

Bone marrow reconstitution using a block grafting technique.

Experiments in rabbits with radiation-induced bone marrow aplasia have demonstrated that intramedullary block grafting is a feasible procedure. This technique requires surgical interference and offers no advantage over intravenous reconstitution. We therefore suggest that it has no value in therapeutic bone marrow transplantation.

Animals↗
PubMedDate not supplied

Pain in the head and neck cancer patient: changes over treatment.

The incidence, severity, and location of pain was evaluated in 30 head and neck cancer patients prior to treatment after the first phase of their treatment and upon the completion of treatment. The incidence of pain was relatively high (40%-70%) and tended to increase slightly over treatment. Patients having advanced disease (stage III or IV) had a higher incidence of pain. Pain severity ratings were stable over treatment. Pain was located close to tumor or incision sites, and a trend for patients to report a greater number of pain sites over treatment was noted. While medical status variables (disease stage and site) were found to predict pain status after the initial phase of cancer treatment, initial pain measurements were more likely to predict pain status at the completion of treatment.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Evaluation of a genus-specific monoclonal antibody in an amplified enzyme-linked immunoassay in the detection of Chlamydia in urine samples from men.

In this study, the authors used amplified enzyme-linked immunoassay (IDEIA Chlamydia test) on urine samples of 623 male patients attending a sexually transmitted disease clinic. They compared the results with routine urethral cell culture isolation, which showed a sensitivity and specificity of 72.6% and 98.6%, respectively. The sensitivity was significantly higher (79.7%) in patients with clinical evidence of urethritis as compared with those without such evidence (59%). The use of first-catch, early-morning urine may improve the sensitivity of the test.

Antibodies, Monoclonal↗