PubMed Health⌕ Search

Biomedical subjects

G Marais

Publications and source records attributed to G Marais.

13 recordsLinked to original sources
PubMed2005

Steps in the evolution of heteromorphic sex chromosomes.

We review some recently published results on sex chromosomes in a diversity of species. We focus on several fish and some plants whose sex chromosomes appear to be 'young', as only parts of the chromosome are nonrecombining, while the rest is pseudoautosomal. However, the age of these systems is not yet very clear. Even without knowing what proportions of their genes are genetically degenerate, these cases are of great interest, as they may offer opportunities to study in detail how sex chromosomes evolve. In particular, we review evidence that recombination suppression occurs progressively in evolutionarily independent cases, suggesting that selection drives loss of recombination over increasingly large regions. We discuss how selection during the period when a chromosome is adapting to its role as a Y chromosome might drive such a process.

Animals↗
PubMed2004

Effects of spontaneously chosen crank rate variations on electromyographic responses in sub-maximal arm exercise in inexperienced subjects.

The aim of the present study was to compare electromyographic responses during arm exercises with a crank rate chosen spontaneously ( T(S)) or set at 20% below or above ( T(-20), T(+20)) the spontaneously chosen crank rate (SCCR). Ten male physical education students performed arm exercises with intensities ranging from 20% to 80% of maximal power. Muscular activity levels were analysed for the biceps brachii and the triceps brachii muscles using integrated rectified surface electromyography (iEMG). All values were presented as the mean and standard deviation. During T(S), the sum of iEMG for the two muscles studied was significantly ( P<0.05) lower than during T(+20) for each power output. No significant differences were observed in iEMG values between T(S) and T(-20). The hypothesis that SCCR relates to a minimisation of muscle activation during an upper body exercise was not confirmed. Variations superior or inferior to a 20% increase of the iEMG responses do not influence it. Moreover, the selection of crank rates depends on the power output and the SCCR increased significantly ( P<0.05) with increasing power output.

Adult↗
PubMed2004

Recombination and base composition: the case of the highly self-fertilizing plant Arabidopsis thaliana.

BACKGROUND: Rates of recombination can vary among genomic regions in eukaryotes, and this is believed to have major effects on their genome organization in terms of base composition, DNA repeat density, intron size, evolutionary rates and gene order. In highly self-fertilizing species such as Arabidopsis thaliana, however, heterozygosity is expected to be strongly reduced and recombination will be much less effective, so that its influence on genome organization should be greatly reduced. RESULTS: Here we investigated theoretically the joint effects of recombination and self-fertilization on base composition, and tested the predictions with genomic data from the complete A. thaliana genome. We show that, in this species, both codon-usage bias and GC content do not correlate with the local rates of crossing over, in agreement with our theoretical results. CONCLUSIONS: We conclude that levels of inbreeding modulate the effect of recombination on base composition, and possibly other genomic features (for example, transposable element dynamics). We argue that inbreeding should be considered when interpreting patterns of molecular evolution.

Arabidopsis↗
PubMed2002

Spontaneously chosen crank rate variations in submaximal arm exercise with inexperienced subjects. Effects on cardiorespiratory and efficiency parameters.

The aim of the present study was to compare the physiological responses during arm exercises when the crank rate was chosen spontaneously (TS) or set at +/- 20 % (T-20, T+ 20) of the spontaneously chosen crank rate (SCCR). Eight physical education male students, aged 22 +/- 3.2 years, performed an upper body exercise in which intensities ranged from unload to 80 % of maximal power. No significant difference was observed in oxygen uptake, ventilation, gross and net efficiency values between TS and T+ 20 or T-20. Nevertheless, oxygen uptake and ventilation were significantly (p < 0.05) lower and gross and net efficiencies higher (p < 0.05) during T-20 than T+ 20. No significant difference was noticed for heart rate, delta and work efficiency between TS, T-20 and T+ 20. The hypothesis that SCCR is the most economical one according to the efficiency parameters was not quite verified. However, crank rates lower than SCCR could be interesting because they increase gross efficiency compared to higher crank rates. Moreover, the selection of crank rates depends on power output. Indeed, SCCR increased significantly (p < 0.05) with power output. In the physical reconditioning of injured or handicapped subjects, the latter are very sensitive to the power output, and the crank rate could be another constraint.

Adult↗
PubMed2002

Plasma lactate recovery from maximal exercise with correction for variations in plasma volume.

BACKGROUND: To compare plasma lactate concentrations and plasma lactate kinetics during recovery, for measured and corrected values for changes in plasma volume, after a maximal aerobic exercise. METHODS: Sixteen male subjects performed an incremental and maximal exercise in order to reach maximal aerobic power. Prior to the exercise, at the end and during recovery (2, 5, 12 and 30 min), blood samples were collected through an antecubital catheter. Samples were analysed for lactate, hematocrit and hemoglobin in order to calculate changes in plasma volume. Plasma lactate concentrations ([La]p) were corrected for changes in plasma volume. Plasma lactate kinetics was estimated through the ratio between [La]p after 5 min recovery minus [La]p after 30 min to time (25 min) and expressed in percentage per minute. RESULTS: Maximal changes in plasma volume (-19.7 +/- 3.8%) were correlated to maximal measured [La]p (r=0.66, p<0.01). Maximal measured [La]p values (14.9 +/- 2.6 mmol x l-1) were 17.3% higher (p<0.001) than corrected values (12.7 +/-2.0 mmol x l-1). The kinetics of [La]p decrease was significantly higher (p<0.001) for measured values (2.38 +/- 0.29 % x min-1) than for corrected values (2.22 +/- 0.33 % x min-1). CONCLUSIONS: These results suggested that changes in plasma volume must be taken into account when peak postexercise plasma lactate concentration or lactate recovery curves are analysed.

Adult↗
PubMed2001

Does recombination improve selection on codon usage? Lessons from nematode and fly complete genomes.

Understanding the factors responsible for variations in mutation patterns and selection efficacy along chromosomes is a prerequisite for deciphering genome sequences. Population genetics models predict a positive correlation between the efficacy of selection at a given locus and the local rate of recombination because of Hill-Robertson effects. Codon usage is considered one of the most striking examples that support this prediction at the molecular level. In a wide range of species including Caenorhabditis elegans and Drosophila melanogaster, codon usage is essentially shaped by selection acting for translational efficiency. Codon usage bias correlates positively with recombination rate in Drosophila, apparently supporting the hypothesis that selection on codon usage is improved by recombination. Here we present an exhaustive analysis of codon usage in C. elegans and D. melanogaster complete genomes. We show that in both genomes there is a positive correlation between recombination rate and the frequency of optimal codons. However, we demonstrate that in both species, this effect is due to a mutational bias toward G and C bases in regions of high recombination rate, possibly as a direct consequence of the recombination process. The correlation between codon usage bias and recombination rate in these species appears to be essentially determined by recombination-dependent mutational patterns, rather than selective effects. This result highlights that it is necessary to take into account the mutagenic effect of recombination to understand the evolutionary role and impact of recombination.

Animals↗
PubMed2001

Synonymous codon usage, accuracy of translation, and gene length in Caenorhabditis elegans.

In many unicellular organisms, invertebrates, and plants, synonymous codon usage biases result from a coadaptation between codon usage and tRNAs abundance to optimize the efficiency of protein synthesis. However, it remains unclear whether natural selection acts at the level of the speed or the accuracy of mRNAs translation. Here we show that codon usage can improve the fidelity of protein synthesis in multicellular species. As predicted by the model of selection for translational accuracy, we find that the frequency of codons optimal for translation is significantly higher at codons encoding for conserved amino acids than at codons encoding for nonconserved amino acids in 548 genes compared between Caenorhabditis elegans and Homo sapiens. Although this model predicts that codon bias correlates positively with gene length, a negative correlation between codon bias and gene length has been observed in eukaryotes. This suggests that selection for fidelity of protein synthesis is not the main factor responsible for codon biases. The relationship between codon bias and gene length remains unexplained. Exploring the differences in gene expression process in eukaryotes and prokaryotes should provide new insights to understand this key question of codon usage.

Animals↗
PubMed2001

RPE responses during arm and leg exercises: effect of variations in spontaneously chosen crank rate.

The aim of this study was two-fold. First, the rating of perceived exertion (RPE) was compared between two different upper and lower body exercises. Subjects (n = 12) performed with spontaneously chosen crank or pedal rates: (i) incremental maximum power tests (Test 1), with an initial work rate of 50% of maximal power followed by increases of 10% at each 120-sec. work stage and (ii) tests (Test 2) with exercise bouts set at 20, 40, 60, and 80% of maximal power separated by passive recovery periods. Second, the effects of variations in spontaneously chosen crank rate on RPE was analysed using the second test performed only with upper body. Subjects performed Test 2 three times with crank rates spontaneously chosen by the subjects, set at plus or minus 20% of spontaneously chosen crank rate. During both Tests 1 and 2 for upper or lower body, RPE increased linearly (p<.01) with power output. No significant difference was noticed between upper and lower body tests; however, RPE was significantly different (p<.05) between Test 1 results for upper and lower body at 70, 80, 90, and 100% of maximal power. The greater RPE at high power output could be linked to the important effect of fatigue during upper body exercise. Among the three crank-rate conditions, no significant difference in RPE was noticed. The choice of crank rate does not seem to influence the perception of exertion in upper body cycling exercise.

Adult↗
PubMed2000

Transposons but not retrotransposons are located preferentially in regions of high recombination rate in Caenorhabditis elegans.

We analyzed the distribution of transposable elements (TEs: transposons, LTR retrotransposons, and non-LTR retrotransposons) in the chromosomes of the nematode Caenorhabditis elegans. The density of transposons (DNA-based elements) along the chromosomes was found to be positively correlated with recombination rate, but this relationship was not observed for LTR or non-LTR retrotransposons (RNA-based elements). Gene (coding region) density is higher in regions of low recombination rate. However, the lower TE density in these regions is not due to the counterselection of TE insertions within exons since the same positive correlation between TE density and recombination rate was found in noncoding regions (both in introns and intergenic DNA). These data are not compatible with a global model of selection acting against TE insertions, for which an accumulation of elements in regions of reduced recombination is expected. We also found no evidence for a stronger selection against TE insertions on the X chromosome compared to the autosomes. The difference in distribution of the DNA and RNA-based elements along the chromosomes in relation to recombination rate can be explained by differences in the transposition processes.

Animals↗
PubMed1999

Relationship in humans between spontaneously chosen crank rate and power output during upper body exercise at different levels of intensity.

The aim of this study was to assess the relationship between spontaneously chosen crank rate (SCCR) and power output during two upper body exercise tests: firstly, an incremental maximal aerobic power test (T1), with an initial intensity of 50 W followed by 15-W increases at each subsequent 90-s stage and secondly, a test (T2) with consecutive exercise periods set at 50%, 60%, 70%, 80%, 110% and 120% of maximal power (Pmax) separated by passive recovery periods. Eight nationally and internationally ranked kayakers, aged 20 (SD 2) years, performed the tests. During both T1 and T2, mean SCCR values were correlated (r = 1) and increased significantly (P < 0.05) in association with the increases in power output. The finding that the subjects consistently increased their crank rate as the power output increased in different tests, i.e. at submaximal, maximal and supramaximal intensities, strongly suggests that SCCR depended on power output and not on the type of exercise (incremental or rectangular exercise). Moreover, the equation relating crank rate and power output determined from T1 suggests that it may be used to predict the crank rate which will be chosen in upper body exercise, whatever the intensity. Finally, the results of testing at 110% and 120% of Pmax would suggest that a high crank rate (>90 rpm) should be used during the test procedure using supramaximal exercises where accumulated oxygen deficit is calculated, and more particularly when exercise is performed using the upper body.

Adult↗
PubMed1999

Physiological effects of variations in spontaneously chosen crank rate during sub-maximal and supra-maximal upper body exercises.

The aim of the present study was to compare the physiological responses when the crank rate was chosen spontaneously (Ts) or set at +/- 10% (T-10%, T+10%) of the freely chosen rate, during two upper body exercises: i) a sub-maximal test (T(SUB)) in which intensities ranged from 50 to 80% (118.4 +/- 10.2 to 189.5 +/- 16.3 watts) of maximal power (MP) and ii) a supramaximal test (T(SUPRA)) in which power output was set at 110 and 120% (260.5 +/- 22.4 and 284.2 +/- 24.4 watts) of MP. Eight nationally and internationally ranked kayakers, aged 20 +/- 2 years, performed these tests in which power outputs were normalised in relation to the maximal power output determined during T(MP). In T(SUB+10%), oxygen uptake and ventilation were significantly (P< 0.05) higher than during T(SUBxS). In T(SUB+10%) and T(SUB-10%), energy expenditure was significantly (P<0.05) higher and gross and net efficiencies lower than during T(SUBxS). During T(SUPRA-10%) when the power output was set at 110% of MP, time to exhaustion was significantly higher (P<0.05) than during T(SUPRAxS). The findings of the present study suggest that upper body exercise performed on an ergocycle should be conducted using the freely and spontaneously chosen crank rate.

Adult↗
PubMed1997

Physiological effects of variations in spontaneously chosen crank rate during incremental upper-body exercise.

The aims of the present study were: first, to assess the interindividual variations of a spontaneously chosen crank rate (SCCR) in relation to the power developed during an incremental upper body exercise on an arm ergometer set at a constant power regime, and second, to compare heart rate (HR) responses, expired minute ventilation (V[E]) and oxygen consumption (VO2) when the pedal rates were chosen spontaneously (T[SCCR]) or set at +/- 10% of the freely chosen rates (T[+10%] and T[-10%], respectively). The mean pedal rate values were linearly related (P < 0.01) with the power developed during arm cranking (r = 0.96), although large variations of pedalling rate strategies were observed between subjects. Maximal power (MP) and time to exhaustion values were significantly higher (P < 0.05) during T(SCCR) than during T(+10%) and T(-10%). Peak VO2 values were significantly higher (P < 0.05) in T(+10%) than in T(SCCR) and T(-10%). The increase in HR, V(E), and VO2 mean values, in relation to the increase in the power developed, was significantly higher (P < 0.05) when the pedal rate was set at plus 10% of the SCCR (T[+/-10%]) than in the two other conditions. The findings of the present study suggest that the use of an electromagnetically braked ergometer, which automatically adjusts the resistance component to maintain a constant work rate, should be used in order to achieve the highest MP values during an incremental upper body exercise. A 10% increase of the SCCR should be used in order to provide the highest peak VO2 value.

Adult↗