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Biomedical subjects

G Marinelli

Publications and source records attributed to G Marinelli.

At least 37 records · Page 2Linked to original sources
PubMed1981

Substantial amounts of gonadotrophin-like materials in sera of normal subjects.

Ultrafiltration of human sera resulted in increased levels of human chorionic gonadotrophin (hCH), LH and FSH measured by radioimmunoassay. The effect was greatly enhanced by chromatography of serum through Sepharose-protein A (which specifically binds immunoglobulin G) followed by ultrafiltration of the unretarded fractions. Serum from a normally menstruating women was treated by ultrafiltration and the retained fraction from 200 mul serum further chromatographed on a Sephadex G-150 Superfine column. The amounts of LH and FSH measured in the eluate by radioimmunoassay were higher by three orders of magnitude than those found in the untreated serum. Comparable amounts of hCG were also found in the eluate by two specific radioimmunoassays, and a value of 45 i.u. hCG was recovered in the eluate by bioassay. Serum from another normally menstruating women was applied to DEAE-cellulose and eluted stepwise using an elution gradient. The ultrafiltrate of one eluted fraction led to separation of a low molecular weight material which was able to neutralize the biological activity of a large dose of hCG. The data support the hypothesis that substantial amounts of gonadotrophins are normally present in serum but that binding to inhibitor(s) prevents their assay in untreated sera.

Biological Assay↗
PubMed1977

[The surgery of atrial septal defect in adults (author's transl)].

33 cases of A.S.D. whose age ranged between 30 and 50 years are presented. Surgical indications, post-operative complications and arrhythmias are described. Atrial fibrillation due to pulmonary hypertension in the post-operative period is particularly emphasized. The authors point out that surgical indication for A.S.D. in adults is closely related to the hemodynamic of pulmonary circulation.

Adult↗
PubMedDate not supplied

Microbiological characterization of freatobiont organisms: trial of an analytical protocol and hygienic implications.

The objective of the present study lies in verifing the applicability of an analytical protocol to characterize the microbiology of the surface and gut of freatobiont organisms. The fundamental hypothesis of the study is that the subsurface microinvertebrates represent a protective condition for bacteria, whose presence in potable spring water is rigorously excluded by european standards of quality. The study was conducted between August 1996 and March 1998. Sediment samples were collected from the spring system of "Presciano", whose waters come from the regional aquifer of the Gran Sasso Massif in Italy (L'Aquila, Abruzzo). Drawings of samples were conducted at two different depths (-70 cm and -150 cm). The extration of the fauna (including copepodes) from sediments were achieved with the aid of a Wild M3 microscope. Isolate freatobiont organisms were placed in a sterile Petri plate and subjected to three washings. The liquids of the second and third washings were microbiological analyzed proceeding, in the course of the third washing, to squash the organisms with the aid of a sterile iron handle. Among the microinvertebrates, the copepod Harpacticoida Nitocrella pescei is the most represented species from the two levels of depth. In all, were isolated and identified 106 bacterial species; of these species only 12 (11.3%) did not show any adaptation to the temperature of 35 degrees C. The analytical protocol permitted to make evident a different distribution of microbial species between the two kinds of washing liquids, and this leads to considerate appropriate the adopted protocol. Even if the number of sediment samples analyzed was a few it was identified a great variety of microbial genera and species the greater part of which were mesophilic. This is a consequence of an adaptability that could be favoured by the presence of freatobiont fauna and that concern even microorganisms usually considered "faecal contamination indicators".

Adaptation, Physiological↗
PubMedDate not supplied

[One hundred years old and under haemodialysis treatment for 5 years...a case to ponder].

BACKGROUND: The average age of the patients, introduced in the dialytic programme, is progressively increasing in relation to the prolonging of life and to the technological progresses. All this places the nephrologist to ask himself if the age has to be considered a limiting factor for the introduction of a patient in the dialytic programme. CASE REPORT: We report on a patient who started her dialytic programme when she was 95. After 5 years, at the age of 100, she uneventfully completed 60 months of haemodialytic treatment. Right from the start we decided to use as vascular access a double Tesio catheter, which was never replaced because it was the most appropriate for the patient's age. During the follow-up period the patient was not hospitalised and her clinical condition was satisfactory. DISCUSSION: According to the latest epidemiological and clinical data, this case emphasises the concept that it would be wrong to refuse the haemodialytic treatment to such patients only because of their frailty. Moreover, the lack of either univocal prognostic indications, based on scientific criteria, or special national laws confirm that the decision to treat the patient depends exclusively on the physician's ethical and professional background as well as the patient's autonomy and conscience.

Aged↗
PubMedDate not supplied

[Radiculopathy and the organization of health services: applicability verification of a technic for analyzing time factors in diagnostic procedures].

The PERT is a SPR (Reticular Programation System) based on statistic-mathematic models. Since some years they are applied to productive processes to increase the efficiency and effectiveness. They all have a same base structure which is the net that is composed by the logical succession of the event and the activity which has the part of the project. Determining the minimum time (to), the maximum one (tp) and the more frequent time (tm) of each activity and applying the statistic method PERT, one gets the probable duration (te) of every activity and the critical path of the net is placed in evidence. This is formed by the chain of those activities whose duration determines the total duration of the project. The Authors have desired to verify the applicability of SPR even to the "medical industry" and, more precisely, to the diagnostic process for the verification of radiculoneuropathy. Such pathology affects a large part of the active population. The diagnostic process in this case comprises besides an accurate neurological and clinical examination of the patient, also the x-ray exam, the electromyography, the Computed Tomography (CT) and the Magnetic Resonance Imaging (MR) exam. These last two investigations gives an increase of diagnostic accuracy. With the collaboration of the physicians of the diagnostic Service, the Authors have measured in every step of the diagnostic procedure the waiting time of 48 patients with low back pain. Applying the PERT method, it has been possible to put in evidence in the net the critical activities. They are such that their duration time determines the probable duration of the whole diagnostic process. Such duration in this case it corresponds to 91 days with a standard deviation of 33 days. The delay of any critical activity causes a lealy of the whole route. This delay influences negatively on the patient's health. Besides it determines an economic damage to the system because a relation cost/time exists. The systems of reticular programmation have as objective not only the one of improving the programmation and the control of the processes, but also the attainment of an optimum cost/time ratio, varying in a way that the total cost of the realization of the process is minimum. They represent a useful criterion to direct Quality Assurance (Q.A) in the local political sanitary context, within the bounds of organization of technical - scientific quality. An accurate application of the Q.A. should modify besides the duration of the critical activities.

Adult↗
PubMedDate not supplied

[Type of newborn infant feeding and an epidemiological approach to SIDS].

There are many etiopathogenetic theories that hypothesize several causes or factors supporting the onset of Sudden Infant Death Syndrome (SIDS). Among the SIDS factors there are bacterial endotoxins which can be conveyed into the organism in large amounts, by contaminated milk. An epidemiologic research was carried out on a sample of 258 mothers of children and boys attending some schools of L'Aquila district in 1988. It supplied data on the nursing procedures and about diseases with unknown aetiology related to nursing periods. The results obtained refer to the years 1974-84: 33.6% of sample was normal breast-fed infants. We found increasing percentage values referred to bottle-fed infants the first month of life (45% of sample in 1984); 22.5% of sample was bottle-fed infants only. Cows' milk was less and less used and it reached the 5% value in 1984. A case of hypothetic near-SIDS (0.4%) was found and another case which can be defined at SIDS-risk. It concerns two bottle-fed infants whose milk was diluted with simple drinking water. In addition a case of SIDS in a family was found: she was a girl aged 23 weeks who had begun drinking neat cows' milk only twenty days before the disease.

Animals↗
PubMedDate not supplied

Surgical approach to combined carotids and coronaries lesions.

A preoperative carotid evaluation by a Duplex Scanner ATL Mark V has been carried out in 338 patients candidates to CABG. When a critical lesion was detected a digital angiography was subsequently performed. In 19 patients an operation was done because the coexistence of lesions in the carotids and the coronaries. The indications, the timing of the operations and the surgical approach are discussed. In the Author's minds the combined procedure seems to be advisable.

Carotid Artery Diseases↗