PubMed Health⌕ Search

Biomedical subjects

G Medrano

Publications and source records attributed to G Medrano.

25 records · Page 2Linked to original sources
PubMedDate not supplied

Metabolic enzymatic activities in the diaphragm of normal men and patients with moderate chronic obstructive pulmonary disease.

The activities of hexokinase (HK), lactate dehydrogenase (LDH), citrate synthase (CS) and 3-hydroxyacyl-CoA-dehydrogenase (HAD) were measured in the costal diaphragm (DI) and in the latissimus dorsi (LD), a non respiratory muscle, of 21 middle aged men: 10 normal subjects and 11 patients with moderate chronic obstructive pulmonary disease (COPD). In the normal subjects, all the enzymatic activities were significantly higher in the diaphragm (p less than 0.01) than in the latissimus dorsi, the mean increase ranging from 61 to 118%. Chronic obstructive pulmonary disease failed to cause any further increase in enzymatic activities in the DI; on the contrary, a significant decrease of HK and LDH (30%, p less than 0.01) was observed. Enzymatic activities of LD were unaffected by COPD. These data show that the metabolic enzymatic activities in the diaphragm of normal middle aged subjects is similar to that of the vastus lateralis of highly trained young athletes, suggesting that DI is a highly trained muscle in normal man.

3-Hydroxyacyl CoA Dehydrogenases↗
PubMedDate not supplied

Suppression of bronchoalveolar lymphocyte antibody secretion by alveolar macrophage: an in vitro study in the rabbit.

The effect of rabbit alveolar macrophage (AM) on the antibody secretion of bronchoalveolar lymphocytes was investigated in vitro, using a plaque-forming cell assay. Animals were intratracheally primed and reimmunized with 10(10) sheep red blood cells (SRBC). Free alveolar cells were obtained by lung lavage and broncho-alveolar lymphocyte-enrichment was achieved through Sephadex G-10 columns. Cell suspensions with various macrophage-to-lymphocyte ratios (AM:L from 1:25 to 3:1) were prepared using appropriate numbers of plastic adherent alveolar cells. After a 4 day in vitro co-culture of 5 X 10(6) alveolar cells (AM plus lymphocytes) with 3 X 10(6) SRBC, IgM and IgG antibody-forming cells (AFC) were counted. A significant suppressive effect (p less than 0.05) of AM on both IgM and IgG-AFC was observed when AM:L ratio was increased from 1:25 to 1:10. This effect was slightly accentuated with higher AM:L values, required viable AM, and was not affected by adding indomethacin in the culture medium. Moreover, a preliminary co-culture of AM with bronchoalveolar lymphocytes and the antigen was necessary for the expression of this suppressive effect. These data suggest that the suppressive activity of primed AM on alveolar lymphocytes may be of physiologic significance in vivo in the regulation of one of the pulmonary immune responses to airborne antigens, namely local antibody production.

Animals↗
PubMedDate not supplied

[Idiopathic mitral subvalvular aneurysm. Report of a case and review of the literature].

An unusual type of annular, subvalvular left ventricular aneurysm is described for the first time in a 64 years-old mexican woman. The pathological characteristics are similar to those cardiac aneurysms of unknown etiology described in African negroes. In this paper, we review the clinical features, diagnostic difficulties and discuss possible etiologic factors related to this condition.

Female↗