G Mernitz | PubMed Health

PubMed1996

Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis.

The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA of A. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.

Base Sequence↗

PubMed1984

[Right ventricular hemodynamics and lung function following amrinone injection].

UNLABELLED: Amrinone is a positive inotropic agent which has been widely used for treatment of left heart failure. As this drug has a relaxing effect on smooth muscle cells, we considered that hemodynamics and ventilatory mechanics could be simultaneously improved in patients with right heart failure caused by obstructive lung disease. Swan-Ganz catheters were used in 11 patients. Following injection of 1.5 mg/kg Amrinone, cardiac output increased from 6.1 to 6.9 l/min (p less than 0.05). Stroke volume showed only a slight increase; pulmonary vascular resistance was reduced from 407 to 308 dynes . s . cm-5 (p less than 0.01). Body plethysmographic examination revealed a decrease of specific airway resistance from 33 to 24 cm H2O . s (p less than 0.01). CONCLUSION: In patients with right heart failure due to obstructive lung disease Amrinone application can result in an improvement in both hemodynamics and ventilatory mechanics.

Airway Resistance↗