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Biomedical subjects

G Mu

Publications and source records attributed to G Mu.

8 recordsLinked to original sources
PubMed2006

Nonlinear imaging of embedded microstructures inside transparent materials with laser-induced ionization microscopy.

We present a novel nonlinear imaging method that utilizes femtosecond laser-induced plasma emission to probe microscopic structures embedded inside transparent materials. This nonlinear diagnostic tool can resolve either elemental or structural variations of the sample of interest and provide significant improvements over the ordinary linear microscopes by having much higher contrast ratios for the observed areas of different refractive indices. Examples of using this technique to examine the microstructures fabricated by ultrashort laser pulses inside optical glass are presented.

Journal Article↗
PubMed2001

Detection of human herpesvirus 8 DNA in acute leukemia patients.

OBJECTIVE: To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemia (AL) patients. METHODS: The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined using polymerase chain reaction (PCR). Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included. RESULTS: HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AML). The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive. None of the normal donors and human hematopoietic cell lines showed evidence of HHV-8 DNA. CONCLUSION: The results suggest that the prevalence of HHV-8 is low in AL in China.

Acute Disease↗
PubMed2000

[Relationship between human herpesvirus 6 infection and idiopathic thrombocytopenic purpura].

OBJECTIVE: To investigate the pathogenic role of human herpesvirus 6 (HHV-6) in idiopathic thrombocytopenic purpura (ITP). METHODS: HHV-6 DNA was examined by polymerase chain reaction (PCR) in bone marrow mononuclear cells (BMMNC) of 105 ITP patients. Human cytomegalovirus (HCMV) and parvovirus B(19) DNA were also examined in some cases. Platelet-associated antibodies (PAIg) were measured by competitive ELISA in 66 ITP patients. Serum IgG titer to HHV-6 was observed by indirect immunofluorescence assay in 19 ITP patients. RESULTS: (1) HHV-6 DNA positivity was 41.0% for ITP patients, significantly higher than that for control group (P < 0.05). HHV-6 DNA positivity for adult ITP patients, especially adult chronic cases was significantly higher than that for childhood patients (P < 0.025). Positivities for parvovirus B(19) and HCMV DNA were 24.1% and 9.7%, respectively. (2) HHV-6 DNA positivity for patients with abnormal level of PAIgG was significantly higher than that for patients with normal level of PAIgG (P < 0.05). (3) Patients coinfected with HHV-6 and B(19) or HCMV had more severe symptoms or poorer prognosis. CONCLUSION: HHV-6 infection might be associated with excessive PAIgG. Coinfection with HHV-6, B(19) or HCMV may cause more severe symptoms in ITP patients.

Adolescent↗
PubMed1997

Seroprevalence of human herpesvirus-6 in healthy population in two provinces of north China.

BACKGROUND: Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with seroprevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population, this seroepidemiological study was conducted. METHODS: Sera were collected from 430 healthy persons and donors living in North China, and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer equal or higher than 1:10 was regarded as seropositive. RESULTS: Of the 430 serum samples tested, 297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males, with X2 = 7.05, P < 0.01 and F = 7.23, P < 0.01, respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes, with X2 = 20.08 and 20.28, P = 0.04, respectively, and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes. CONCLUSIONS: This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China, and its importance should be further studied.

Adolescent↗
PubMed1997

Detection of human herpesvirus 6(HHV-6) DNA in salivary glands by the polymerase chain reaction.

To assess the presence of HHV-6-Specific DNA in human salivary glands, eighteen specimens of salivary gland tissue were investigated using the polymerase chain reaction. Eight of nine parotid glands, five of seven submandibular glands and one of two sublingual glands were found to have amplification of the HHV-6-specific sequence. The findings suggest that salivary gland tissue is one of the potential sites for HHV-6 persistence following primary infection and that saliva is a vehicle for transmission of the virus.

DNA, Viral↗
PubMed1995

[Interspecific protoplast fusion between Bacillus thuringensis Bt-3701 and Bacillus megaterium Bm-107].

The results of the interspecific protoplast fusion between B. thuringensis sub. kurstaki Bt-3701 which has pesticide ability, and B. megaterium var. phosphaticum Bm-107 which has decomposing phosphate activity, were reported. High frequency of protoplast formation and regeneration was obtained with 4h activated Bm-107 treated by 100 micrograms/ml lysozyme, and with 2h activated Bt-3701 treated by 3% glycin and mild temperature. Using 40% PEG and 5% nascent Ca2+ to treat the parential protoplast mixture for 3 min at 37 degrees C, 4 stable fusants were obtained. Biological tests show that they have both pesticide ability and decomposing phosphate activity, but which are weaker than that of parential strains.

Bacillus megaterium↗