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G N Godson

Publications and source records attributed to G N Godson.

At least 91 records · Page 5Linked to original sources
PubMed1974

Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.

A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.

Alleles↗
PubMed1973

Isolation and partial characterization of temperature-sensitive mutants in ten loci that affect cell membrane synthesis in Escherichia coli: isolation and genetic sorting.

Four hundred temperature-sensitive (ts) mutants were isolated by the (3)H-glycerol-3-phosphate membrane suicide procedure of Cronan, Ray, and Vagelos and were sorted into 13 groups by the rapid mapping procedures of Low. Recombination and complementation studies on representative members of each group suggested that the ts mutations of all 13 groups are present in genetically different complementation groups. Biochemical studies suggested that 10 of these ts mutations affected cell membrane synthesis. In this paper, the genetic data are presented in detail so that the limits of the Low rapid mapping procedures can be assessed, and in an accompanying paper the partial biochemical characterization of the ts mutations is described.

Cell Membrane↗