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Biomedical subjects

G N Moskovkin

Publications and source records attributed to G N Moskovkin.

22 records · Page 2Linked to original sources
PubMedDate not supplied

[Muscarinic-acetylcholine and beta-adrenaline receptors of the retinal and pigment epithelium cells during eye regeneration in tritons].

The development of M-choline and beta-adrenoceptors of retina and pigment epithelium cells has been studied in regenerating newt eye by radioligand analysis. The data obtained have been compared with the histological data. High content of M-choline and beta-adrenoceptors has been observed both in retina, in pigment epithelium of retina-ectomized and control eyes. 7-10 weeks after the operation, incomplete receptors have been observed in the regenerate with incomplete differentiation: they had low binding constants and had non-characteristic bending saturation curves. Pigment epithelium cells could interact with ligands properly. 10-13 weeks after the operation, the values and pattern of binding became similar to that of control retina. The data obtained can be explained by gradual increase in the share of morphologically differentiated cells in retina regenerate.

Animals↗
PubMedDate not supplied

[Adrenoreceptive structures in the early embryos of the clawed toad (Xenopus laevis)].

Studies have been made on the binding of labeled ligands to beta-adrenoreceptor--3H-dihydroalprenolol (DHA) and 125I-iodocyanopindolol (ICP--in crude homogenate and subcellular fractions of toad embryos during cleavage divisions. Specific binding of these ligands was observed which is confirmed by a decrease of the activity after addition of cold propranolol or adrenalin. The value of Kd for DHA is equal to 3 x 10(-9) M for ICP--to 1.5 x 10(-9) M. In addition to this high-affinity pool of binding with a low capacity, a low-affinity and high-capacity pool was also found. Cooling of the preparation up to 4 degrees C for 2 h increases the specific binding simultaneously with a decrease of the affinity of adrenoreceptive structures of the embryo. The data obtained are discussed in relation to the criteria of intracellular transmitter process.

Animals↗
PubMedDate not supplied

[The characteristics of the binding of M-cholinergic blockers and beta-adrenoblockers by the brain synaptosomes of 15-day-old and adult rats].

Significant difference in values of basic parameters, Bmax and Kd which characterize binding of 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol, specific blockers of m-cholino- and beta-adrenoreceptors, has been established for brain synaptosomes from 15-day and adult rats. It was shown that phospholipase A2 (1 microgram/ml), a membrane active component from the venom of the cobra Naja naja oxiana, reduced Bmax and Kd for binding 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol by synaptosomes. It is suggested that functional modifications in membrane during animal development or induced by phospholipase A2 result in shifts in the specific activity of adreno- and cholinoreceptors and, as a result, in disturbances of adrenergic and cholinergic responses.

Adrenergic beta-Antagonists↗