Biomedical subjects
Publications and source records attributed to G N Zatsepina.
An investigation was made of fundamental biophysical principles of the intrinsic electric system functioning in different conditions. This system was shown to govern the vital functions of separate cells and of the entire live organism. We developed a method enabling the correction of an affected intrinisic electric system. This method is based on the application of an external electric current.
Unloading of the hindlimbs' weight--bearing muscles induced considerable changes in animal's static electrical field on the 5th--8th day of experiment.
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It is shown that carcinogenesis can be identified from the first day of development of the process against the background of inflammation response by measuring the electric resistance of the peritoneum.
Special features of changes in human electrical field in some diseases were studied. Methods for the correction of the disease-induced changes in the intrinsic human field using an external current generator were developed.
Seasonal changes of the constant electric field of healthy people of various age groups were studied. The constant electric field was shown to be characterized by a fine structure of distribution of electric potential differences (EPD) along the skin in relation to a referent point located on the neck in the intersection region of trapeziform and clavicular-nipple muscles. The constant electric field of the people of all ages undergoes seasonal changes involving displacement of all EPD values to the positive region during autumn-winter period as compared to the spring-summer one. It is suggested that such EPD change is conditioned by a change of the organism metabolic activity. The discovered differences in EPD displacement values of various age groups can serve as physiological characteristics of transitional processes in the growing organisms.
The kinetics of adaptation of the volume of T-lymphocytes and their membrane potential in a hypotonic medium is investigated. It is shown to have a quasiperiodic character. The shortest adaptation period T = 2 min characterizes the adaptation of T-lymphocytes test animals, the most complete adaptation of the volume and the membrane potential in hypotonic medium. Upon adaptation their values are only 6% higher than respective values in an isotonic medium. If an aseptic inflammatory process develops in a mammalian organism, the adaptation period increases to T = 2.5 min, while the volume and the membrane potential remains 28% higher than in an isotonic medium. In the course of development of induced cancerogenesis, 10 min after the cells have been placed in a hypotonic medium, their size is equal to that at the point of maximal swelling, while the membrane potential is 20% lower than that in an isotonic medium. The reason for the varying adaptability of T-lymphocytes is, in our opinion, that, as the inflammation develops, the concentration of corticosteroids increases, the concentration of intracellular K+ decreases, the concentration of macromolecules increases and the potential of the plasmatic membrane of the cell rises, thus leading to the slowing down of the process of adaptation and its decreased extent. The lack of regulation of the volume by T-lymphocytes of mammals in the organisms of which cancerogenesis develops, testifies to the fact that, in such a case, cells lose their adaptability to changing external conditions. Therefore, the disappearance of adaptability of T-lymphocytes on the third day of development of cancerogenesis represents an early test of the process.
Present work shows that changes in the surface potential (SP) are linearly connected with alterations of the cell membrane potential (MP) at three physiological states of the organism: control (C), induced cancerogenesis (IC) and total reaction of the organism to damage (TRD). In the control greater SP changes are corresponded with the least MP changes. When SP is changed almost by 100%, MP change is about 20%. In the course of TRD development a reverse relationship is observed: 20% change of the charge is corresponded by a practically 100% change of MP. The relationship indices of MP and SP change in the course of TRD and in the control are equal: with a decrease of MP the lymphocyte SP is also decreased. At the initial stages of IC development the relationship index of MP with SP is changed. In this case a 20% decrease of SP is accompanied by a 60% increase of MP.
It has been shown that due to the effect of helium-neon laser-633 nm a variable potential propagates along the human basal complex. The duration of the signal leading front equals approximately 1 sec, while that of the trailing one approximately 3 sec, the signal amplitude approximately 60 mV. As a result of the depolarization signal propagation the membrane complex is depolarized for a long time approximately (30-60 min). Thus it is shown that the laser ray acts as a damaging agent on the basal complex--the control system of living processes.
The authors' findings helped develop schedules of osteogenesis stimulation in cases when this process was shifted towards resorption (periodontosis), as well as develop a method for determining periodontitis stage and the ability of the body to regeneration.
Studies were carried out of changes of NMR-relaxation of water protons bound to blood plasma proteins of linear animals in the course of induced cancerogenesis and total reaction of the organism to damage. It has been shown that the ratio between the times of spin-lattice and spin-spin relaxation can serve as a criterion of the development of induced cancerogenesis at histologically determined stage of its development.
Fine structure of distribution of electric potential difference (EPD) in human skin of some points on arms, body and face relative to a referent point on the neck was investigated. Diurnal variations of EPD fine structure for individuals and seasonal differences expressed in an increase of the amplitude of variations and shift of EPD mean values to the positive region in winter as compared with summer were observed. The indicated peculiarities are connected with external factors and reflect changes of metabolic activity of organism living activity processes.
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The paper states that restraint of an animal causes transition from one quasistationary level of the static electric field to another.
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An ability to measure the electric conductivity of tissue in physiological potential gradient is proposed. It has been shown that the resistance of plant leaf during the development of the injured potential is constant.