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G Naevdal

Publications and source records attributed to G Naevdal.

4 recordsLinked to original sources
PubMed1994

Genetic variation in the hooded seal, Cystophora cristata, based on enzyme polymorphism and multi-locus DNA fingerprinting.

The genetic population structure of hooded seal, Cystophora cristata, was examined by electrophoretic analysis of allozymes and with multilocus DNA fingerprinting. Samples were collected in the Jan Mayen area and off Newfoundland. Allele products were resolved by isoelectric focusing. Only five of 32 protein-coding loci investigated were polymorphic at the 95% level. The proportion of polymorphic loci was estimated to P = 0.233, and average heterozygosity to H = 0.047. Tissue distribution, genotype distribution, and approximate pI (4 degrees C) of the proteins are reported. The allele frequencies of the AAT-2, GPD-2, and GPI-1 loci, were used in genetic comparisons of samples from the two stocks. Chi-square and G-tests showed no significant difference among the samples from the two groups. Highly variable profiles of HaeIII, HinfI and MboI digested genomic DNA were revealed using the human minisatellites 33.15 and 33.6 (HinfI digests only) as hybridization probes. Comparisons of band-sharing coefficients from HinfI and MboI digest were carried out. We were unable to detect significant differences in band-sharing between Newfoundland and the Jan Mayen area. The hypothesis that there is a considerable degree of intermixing between the stocks cannot be rejected.

Animals↗
PubMed1994

Using isoelectric focusing to discern enzyme variation in northeast Atlantic stocks of the harp seal (Phoca groenlandica).

Liver and/or muscle samples were collected from harp seals on the breeding and moulting grounds in the White Sea area (N = 316) and in the Greenland Sea (N = 225). Of a total of 25 presumptive loci detected by isoelectric focusing on polyacrylamide gels (IFPAG), 11 were variable. Of these, 4 were polymorphic at the 95% level, another 2 at the 99% level. The study shows that the higher resolving capacity obtained by IFPAG, as compared to starch gel electrophoresis, increases the number of electrophoretically detectable polymorphic loci in this species. The paper also compiles a set of methodical modifications which adapt IFPAG to large scale multilocus screening. The study indicates no marked differences between the White Sea and Greenland Sea stocks in frequency distributions of the consistently resolved enzyme systems. Other evidence suggests that potential exchange of genetic material between Northeast Atlantic harp seal stocks may still be so limited that for management purposes the stocks may be regarded as separate and self-sustaining units.

Animals↗