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Biomedical subjects

G Nanni

Publications and source records attributed to G Nanni.

At least 109 records · Page 6Linked to original sources
PubMed1984

Induction of cytochrome(s) P450-dependent drug metabolism in cultured MH1C1 hepatoma cells.

A cell line derived from a Morris hepatoma, MH1C1, was examined for its in vitro expression of monooxygenases. These cells were found to contain different forms of cytochrome P450, as shown by the response to inducers, namely phenobarbital (PB), 3-methylcholanthrene (MC) and metyrapone (MP). MH1C1 cell monolayers exposed to PB or MC showed an increase in the concentration of two spectrally distinct forms of cytochrome P450. The PB and MC treatments elicited enzyme activities towards the substrates aminopyrine and benzo(a)pyrene, respectively. The cell treatment with metyrapone led to a simultaneous stimulation of aminopyrine demethylase and benzo(a)pyrene hydroxylase activities, so underlining the peculiar features of this inducer.

Animals↗
PubMed1984

A 10% mannitol solution effective for colonic preparation for X-ray.

Hydroelectrolytic changes, the time of action and complications induced by oral administration of 10 per cent mannitol solution in the colonic preparation for X-ray examination were examined. Twenty-six patients were examined using two different solution volumes: one liter in 15 patients and two liters in 11 patients. No significant biochemical changes were noted. X-ray study of the colon revealed an insufficient evacuation in 3.8 per cent, sufficient in 7.6 per cent, and good in 88.6 per cent of cases. In 6 per cent nausea and vomiting occurred, in 11.7 per cent abdominal distention, and in 7.6 per cent hypotension. This approach was well tolerated, particularly when one liter of the solution was used.

Barium Sulfate↗
PubMed1984

Phospholipids, vitamin A and ubiquinone of the Golgi apparatus subfractions from rat liver after acute ethanol intoxication.

Previous investigations from our laboratory have shown that during acute ethanol intoxication the Golgi apparatus seems involved in impaired dismission of lipoproteins. In the present paper the phospholipid distributions of Golgi subfractions have been analyzed in livers of normal and ethanol intoxicated rats. No significant differences in the phospholipid classes have been observed in this study. On the contrary, a decrease of vitamin A and ubiquinone in Golgi subfractions is evident. The results are briefly discussed in view of the role played by these endogenous antioxidants in the Golgi membranes and in the pathogenesis of ethanol induced fatty liver.

Alcoholic Intoxication↗
PubMed1984

Increased lipid fuel dependence in the critically ill septic patient.

The effect of sepsis in modifying post-surgical fuel utilization in critically ill patients was determined from 374 observations (246 septic [S] and 128 nonseptic [N] in 12 intubated ICU patients studied serially. Patients received TPN (values/24 hrs: Septic, N2, 9.1 +/- 2.2 gm; glucose, 543 +/- 211 kcal/m2, Nonseptic, N2, 8.3 +/- 3.6 gm; glucose, 550 +/- 346 kcal/m2). In some periods, intravenous lipid (L) was given to raise total caloric intake to 826 +/- 223 kcal/ 24 hr/m2. The VO2, VCO2, respiratory rate, minute volume, and blood gas levels were measured, and respiratory quotient (RQ) and metabolic rate (MR) computed. Statistics were performed by 2-way ANOVA and analysis of covariance. Without lipid, mean VCO2 for S (126 ml/min/m2) and N (128 ml/min/m2) were not significantly different, but VO2 in S (146 ml/min/m2) and N (132 ml/min/m2), and the RQ values S (0.88) and N (0.97), were different (p less than 0.0001). In 360 studies RQ was shown to be increased by the total caloric intake, but reduced in the presence of sepsis: RQ = 0.00014 (kcal/m2) - 0.09 (sepsis effect + 0.878 N = 360; r2 = 0.304; F2,357 = 78; p less than 0.0001; but both administered glucose and lipid calories contribute to the RQ in sepsis: RQ = 0.00017 (glucose kcal/m2) + 0.266 X 10(-3) (lipid kcal/m2) + 0.732 n = 114; r2 = 0.260; F2,111 = 19.5; p 0.0001. Sepsis increased VO2 with little change in VCO2, thus RQ fell, suggesting increased use of lipid fuels for oxidation. During hypercaloric lipid infusion in septic patients (SL) VO2 and VCO2 increased but VO2 was still greater, so RQ remained low (SL RQ = 0.89). As sepsis worsened VO2 remained high but VCO2 fell producing RQ less than 0.8, while plasma glucose levels were increased. These data suggest that septic patients are more dependent than nonseptics on lipid fuels for oxidative metabolism, and that IV lipids can be used to increase oxidative metabolism in sepsis at a time when glucose metabolism appears reduced.

Aged↗
PubMed1984

Retrograde nephrostomy for stone removal: combined cystoscopic/percutaneous technique.

The retrograde percutaneous nephrostomy, a new technique for access to the urinary tract for stone removal, involves cystoscopic placement of a 9 French catheter into a selected minor calix, through which a sheathed 20- or 21-gauge needle is then advanced to exit at the skin. The tract is then dilated over this needle. This technique has been used successfully in 38 patients with only three complications. The technique is more reliable and less time-consuming than the standard antegrade percutaneous approach, particularly in the nondilated pelvicaliceal system. However, to avoid damage to the liver, spleen, or pleural cavity, care must be taken to ensure that the needle does not exit in a cranial direction.

Cystoscopy↗
PubMed1983

Hawkins-Hunter retrograde transcutaneous nephrostomy: a new technique.

Retrograde nephrostomy, a new technique to aid in stone management, has been performed successfully in dogs. It has also been used without complication in a human patient to help remove a renal stone percutaneously under local anesthesia. The technique consists of placing a coaxial catheter over a guidewire under fluoroscopy into the exact calyx desired and advancing a long needle out to the skin to establish a transcutaneous tract. The advantages of the technique include increased control and precision of tract placement, efficient working angles for percutaneous stone removal, and the ability to perform the procedure under local anesthesia.

Animals↗
PubMed1983

Cardiorespiratory and metabolic adequacy and their relation to survival in sepsis.

Cardiorespiratory and metabolic data were collected in 36 septic patients and 80 patients critically ill after nonseptic general surgery or trauma. Septic patients, particularly nonsurviving septics, showed early and sustained increases in metabolic rate, oxygen consumption, cardiac work, and minute ventilatory volume. They had lower levels of respiratory quotient and total peripheral resistance than nonseptic patients. High cardiac work was related to increasing oxygen transport. Both increases in metabolic utilization with high CO2 production, and abnormal increases in VD/VT appeared responsible for the higher minute ventilatory volumes. Respiratory quotient was negatively related to metabolic rate; this relation was modulated by the rate of caloric intake and, in nonseptic patients, was also influenced by the hemodynamic state. These data suggest that poor prognosis in sepsis is characterized by an early sustained stress response with more severe abnormalities in cardiovascular, metabolic, and respiratory function than is seen in the nonseptic stress response. The need for early support of physiologic functions and early and aggressive nutritional intervention is emphasized.

Carbon Dioxide↗
PubMed1982

Ogilvie's syndrome (acute colonic pseudo-obstruction): review of the literature (October 1948 to March 1980) and report of four additional cases.

Four additional cases of Ogilvie's syndrome (acute colonic pseudo-obstruction), representing the first cases described in Italy, are reported. The medical literature concerning the subject is also thoroughly reviewed. Ogilvie's syndrome is an acute massive dilatation of the large bowel without organic obstruction of the distal colon. Three hundred and fifty-one cases have been described in the literature to date. Eighty-eight per cent of the cases were associated with various extracolonic affections (metabolic and organ dysfunctions, postoperative and posttraumatic states, etc.). Twelve per cent of cases were not associated with known disorders and were defined as idiopathic. The pathophysiology of the syndrome is still unknown. Ogilvie, who first described the syndrome in 1948, suggested an imbalance between the sympathetic and parasympathetic innervation of the colon: this neurogenic hypothesis has been shared by other authors, although explanations may differ slightly. The clinical and radiologic picture closely resembles mechanical obstruction of the large bowel. The most marked dilatation usually takes place in the right colon and cecum: if the distended cecum reaches a diameter larger than 9 to 12 cm, perforation is likely to occur; if perforation occurs, the mortality rate increases from 25 to 31 per cent to about 43 to 46 per cent. If conservative management fails to control the dilatation and cecal rupture is impending or suspected emergency surgery is indicated, the surgical procedure of choice is dictated by the general conditions of the patient as well as by the intestinal findings: operation may consist of cecostomy, colostomy, or right hemicolectomy or simply emptying the bowel.

Adult↗
PubMed1982

Analysis of rat liver Golgi apparatus proteins and apolipoproteins in acute ethanol intoxication.

Protein patterns of rat liver secretive (F1, F2) and formative (F3) Golgi fractions and their corresponding VLDLs content have been analyzed by disc-electrophoresis in normal conditions and after acute ethanol intoxication and compared with serum proteins and isolated lipoproteins. The major bands of the normal nascent VLDLs isolated from both formative and secretive Golgi fractions migrate in the same area of serum apo-C and apo-ARP apolipoproteins; in the same density interval of VLDLs another major lipoprotein band, called U, may be separated showing a slower electrophoretic mobility of apo-ARP. After ethanol intoxication apo-C bands seem to be concentrated in secretive and decreased in formative fractions, while apo-ARP and U bands show an overlapping in both Golgi fractions. The possible mechanisms of these behaviours are discussed.

Alcoholic Intoxication↗