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Biomedical subjects

G Nicoloff

Publications and source records attributed to G Nicoloff.

7 recordsLinked to original sources
PubMed1992

Age-related changes in the levels of IgG, IgM and IgA antibodies to type I collagen in the sera of normal human subjects.

Serum samples from healthy subjects of different ages (within the age range of 1-75 years) were tested for the presence of anti-type I collagen (anti-CI) IgG, IgM and IgA by enzyme-linked immunosorbent assay (ELISA). All the tested sera showed anti-CI antibodies from the three immunoglobulin classes with the following age-related regularities: 1. Anti-CI IgG and IgM showed statistically non-significant changes up to the age of 60 decreasing thereafter. 2. Anti-Ci IgA changed negligibly up to the age of 40 increasing with age thereafter. The established age-related changes in the levels of anti-CI antibodies may serve as a basis for future studies of normal and pathological turnover of type I collagen.

Adolescent↗
PubMed1991

Age-dependent changes in the level of antielastin antibodies of different immunoglobulin classes (IgG, IgM, IgA and IgD) in the human serum.

Serum samples from healthy subjects of different ages (within the age range 1-75 years) were tested for the presence of antielastin IgG, IgM, IgA and IgD by an enzyme-linked immunosorbent assay, utilizing insoluble human aortic elastin. All the tested sera showed detectable levels of antielastin antibodies of the four classes with the following regularities in changing their level with age. Antielastin IgG and IgM showed relatively high levels in the serum of children, growing even higher in the serum of subjects 18-20 years old. Then their levels were stabilized in the serum of 30-60 year olds for IgG and of subjects 30-50 years old for IgM and gradually decreased thereafter. The antielastin IgA showed non-significant changes with age up to the age of 40 and then its level gradually increased. The antielastin IgD showed statistically non-significant changes with age and a tendency to decrease after the age of 60.

Adolescent↗
PubMed1989

Immunological investigations on the antigenic changes of human aortic elastin in aging and atherosclerosis.

Alpha-elastin of human aorta was investigated by an enzyme-linked immunosorbent assay (ELISA) on the material isolated from aborted human fetuses and healthy subjects killed by accident, assigned to 7 age groups. Samples up to the age of 55 were taken only from regions without detectable changes in the arterial wall, in the 60-75-year age group--both from normal areas and atherosclerotic plaques of the same aortas. An immune serum against alpha-elastin isolated from the normal aortic areas of a 61-year-old subject was produced in sheep. Testing with this serum showed the existence of some antigenic changes in the elastins from different age groups. A prevalence of the species-specific antigenic determinants was observed in alpha-elastin from fetal aorta while in alpha-elastin from the atherosclerotically altered human aorta the cross-reacting antigenic determinants prevailed in its antigenic structure.

Adolescent↗
PubMed1988

Detection of elastin-antielastin circulating immune complexes (CIC) in diabetic patients with vascular damage.

Healthy subjects aged between 25 and 60 (20 cases) and between 61 and 65 (5 cases), and diabetic patients with vascular damage, aged between 24 and 62 (6 cases), were tested by a new method for the detection and identification of elastin-antielastin circulating immune complexes (CIC) in human sera. Such immune complexes were found in all patients' sera and only in one of the controls (at the age of 65). Among different patients, the elastin-antielastin CIC varied in size and elastin content, showing some correlation between these two characteristics and the existence of microvascular complications, as proved by the clinical and paraclinical investigation of the patients.

Adult↗
PubMed1987

Age-related changes in anti-elastin antibodies in serum from normal and atherosclerotic subjects.

A modified version of the enzyme-linked immunosorbent assay (ELISA), utilising human insoluble aortic elastin, was used for determination of anti-elastin antibodies in serum from normal and atherosclerotic subjects. The age-related changes in their level among healthy persons were investigated. Anti-elastin antibodies were found in all the tested human sera, showing the highest level at the age of 18-20 and the lowest at the age over 60 and especially among atherosclerotic patients. The possible role of the immune system in the turnover of elastin is discussed.

Adolescent↗
PubMed1987

Age-related changes in the level of circulating elastin-derived peptides in serum from normal and atherosclerotic subjects.

The level of the circulating elastin-derived peptides (CEDP) in the serum is believed to reflect the activity of the degradation of the elastic structures. This paper reports a new method, based on the 'sandwich' version of the enzyme-linked immunosorbent assay (ELISA), for the detection and quantification of CEDP in human serum. By this method we investigated the age-related changes in their level among healthy subjects within the age range of 1 and 75 years and among atherosclerotic subjects aged 50 to 75 years. The highest level of CEDP was found in the serum of the atherosclerotic patients, and the lowest, among the healthy subjects between 18 and 50 years of age.

Adolescent↗