Lysozymuria and gentamicin nephrotoxicity.
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Biomedical subjects
Publications and source records attributed to G Nicot.
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The proteinuria of fifteen patients treated with just aminoglycoside or aminoglycoside and either penicillin or cephalosporin was studied. The proteinuria was analysed by means of immunoelectrophoresis, acetate cellulose electrophoresis, thin-layer polyacrylamide gel electrophoresis and sodium dodecylsulphate acrylamide gel electrophoresis. We observed a urinary excretion of free immunoglobulin light chains and an increased urinary excretion of lysozyme in all cases. The increase in urinary excretion of beta-2-microglobulin and retinol-binding-protein appeared only in patients treated with aminoglycoside and cephalosporin. These disturbances disappeared a few days after the treatment was discontinued.
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Studies on red cell arylesterase activity were performed for two years by potentiometric titration using phenylacetate as substrate. From April to October enzymes had higher arylesterase activity than the rest of the year. During that period an increase of arylesterase activity by L-phyenyl-alanine had not been shown.
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