PubMed Health⌕ Search

Biomedical subjects

G Nowicka

Publications and source records attributed to G Nowicka.

At least 37 records · Page 2Linked to original sources
PubMed1987

Influence of bezafibrate and colestipol on LDL-cholesterol, LDL-apolipoprotein B and HDL-cholesterol in hyperlipoproteinaemia.

A significant increase of LDL-apolipoprotein B by 13% and LDL-cholesterol by 19% was observed in a group of 9 patients with hyperlipoproteinaemia Type III after bezafibrate treatment. Additional administration of colestipol caused a significant decrease of both LDL-apolipoprotein B by 18% and LDL-cholesterol by 25%. In 10 patients of hyperlipoproteinaemia Type IIb a significant decrease of both LDL-apolipoprotein B by 28% and LDL-apolipoprotein B by 18% was observed after bezafibrate therapy. When bezafibrate was given together with colestipol a further decrease of both LDL-cholesterol by 17% and LDL-apolipoprotein B by 16% occurred. HDL-cholesterol concentration increased significantly in both groups of hyperlipaemic patients during therapy. This may be the effect of both bezafibrate and colestipol. It is concluded that bile acid resins may effectively prevent the LDL-cholesterol concentration increase observed sometimes after clofibrate analogues.

Adult↗
PubMed1987

The effect of thermally oxidized soya bean oil on metabolism of chylomicrons. Increased uptake and degradation of oxidized chylomicrons in cultured mouse macrophages.

Oral administration of thermally oxidized soya bean oil (TO) increased the level of lipid peroxides in human plasma, mainly in chylomicrons. No changes were observed after fresh oil (FO) intake. Human chylomicrons obtained after TO ingestion were rich in lipid peroxides and degraded more rapidly by cultured mouse macrophages than chylomicrons after FO. The uptake of TO chylomicrons by macrophages occurred via a saturable process and was partially inhibited by beta-very low density lipoprotein as well as by acetyl-low density lipoprotein and fucoidin. A 48-h incubation of macrophages with TO chylomicrons caused a 10-fold higher accumulation of cholesterol ester mass in the cells than the incubation with FO chylomicrons. These studies suggest that chylomicrons containing lipid peroxides may be taken up by mouse macrophages by mediation of beta-VLDL receptor as well as by acetyl-LDL receptor, and show a potential pathway by which chylomicrons obtained after ingestion of heated oil could contribute to accumulation of cholesterol esters in macrophages.

Adult↗
PubMed1987

Hair zinc and copper concentration in survivors of myocardial infarction.

Increased Zn/Cu ratio in the diet, and consequently in the body, was suggested to be of importance in the pathogenesis of atherosclerosis. Head hair of 29 male survivors of myocardial infarction and of 23 control males was studied for the concentration of Zn and Cu. The Zn hair concentration and Zn/Cu ratio in survivors of myocardial infarction was significantly higher in comparison with controls. The inclusion of the Zn/Cu ratio into the discriminant analysis using total cholesterol and HDL cholesterol considerably improved the coefficient R2 and decreased the number of cases not properly classified.

Adult↗
PubMed1984

Serum triglycerides, cholesterol, low density lipoprotein-apo B and high density lipoprotein cholesterol subfractions after moderate body weight loss.

Low calorie diet (4.18 MJ daily) was given to 48 (35 female, 13 male) obese patients during 24 days followed by 7 days of normal diet (9.61 MJ daily). Patients were treated in sanatorium under steady medical control. The average body weight decrease in women was 5.3 kg and in men 6.2 kg. A significant decrease in serum triglycerides, total cholesterol and apolipoprotein B in low density lipoproteins (LDL-apo B) was observed after low calorie diet. No influence on high density lipoprotein cholesterol (HDL-chol), HDL2-chol and HDL3-chol in men and on HDL3-chol in women was observed. The HDL2-chol decreased in women. The LDL-apo B/HDL-chol ratio decreased in both sexes, however, it was significant only in women. After 7 days of normal diet no further significant changes in serum lipids and lipoproteins were observed.

Adult↗
PubMedDate not supplied

Comparative analysis of plasma lipoprotein components assessed by ultracentrifugation in primary biliary cirrhosis and chronic hepatitis.

UNLABELLED: The evaluation of lipid and lipoprotein profiles in liver diseases has important cognitive aspects and provides practical information contributing to the diagnosis of liver pathology. There are few studies of this problem using the ultracentrifugation method. AIM OF THE STUDY: A comparison of lipid profile (obtained by plasma ultracentrifugation) in patients with primary biliary cirrhosis (PBC) and chronic hepatitis (CH). MATERIAL AND METHODS: 103 percutaneous liver biopsies were routinely performed from 1994 to 1997. Blood samples were taken from all the patients at the time of biopsy for further evaluation of lipid profile. 15 patients with PBC and 15 patients with CH (of HBV or/and HCV etiology) were studied. RESULTS: In patients with CH mean total, esterified and free cholesterol levels (166 mg%, 117.6 mg% and 48.1 mg%, respectively) were significantly lower (p < 0.002; p < 0.004; p < 0.006, respectively) than in patients with PBC (237.5 mg%, 165.7 mg% and 71.8 mg%, respectively). The phospholipid concentration in sera of patients with PBC were significantly higher (271.1 mg%, p < 0.0004) than in patients with CH (187.6 mg%), whereas apolipoprotein B and apoAII were significantly lower. Total, esterified and free cholesterol levels in LDL fraction were significantly higher in patients with PBC (175.3 mg%, p < 0.007; 117.9 mg%, p < 0.02; 57.6 mg%, p < 0.01, respectively) than in patients with CH (113.7 mg%, 78.7 mg% and 35 mg%, respectively). The concentration of phospholipids in LDL fraction in patients with PBC was significantly higher (166.8 mg%; p < 0.003) in comparison with patients with CH (96.3 mg%). The differences in other lipoprotein fractions (VLDL and HDL) were not statistically significant. CONCLUSIONS: High levels of total, free and esterified cholesterol as well as phospholipids, apolipoprotein B and AII were observed by us in patients with PBC in comparison with patients suffering from CH. The increase of cholesterol (total, esterified and free) and the phospholipid concentration in serum, are a manifestation of their higher concentration in LDL fraction.

Adult↗
PubMedDate not supplied

Propranolol decreases cholesteryl ester accumulation in mouse peritoneal macrophage in vitro.

Current evidence indicates that the non-selective beta-blocker propranolol reduces the severity of experimental atherosclerosis in animal model. We studied the effect of propranolol on cholesterol ester synthesis and storage in mouse peritoneal macrophages in vitro. Propranolol in concentrations found in the plasma of patients treated with it (150 ng/ml) decreased by 20-30% beta-VLDL-stimulated synthesis of cholesterol esters with (14C)-oleate. A similar effect was observed also on this synthesis stimulated by acetyl-LDL. In a similar degree the accumulation of cholesteryl esters in the cells was decreased during the 48-hour incubation with lipoproteins in the presence of propranolol. No effect of the drug was observed on the uptake and degradation of beta-VLDL or acetyl-LDL. These observations suggest that propranolol may inhibit cholesteryl ester accumulation in macrophages at the point subsequent to lipoprotein uptake. This may have an impact on the treatment or prevention of atherosclerosis.

Animals↗
PubMedDate not supplied

Influence of experimental diets on cholesterol and triglyceride levels of rabbit blood serum lipoproteins.

Groups of rabbits were fed for six weeks various diets: standard died + ethanol, high-cholesterol diet and a high-cholesterol + ethanol one. During the next six weeks every diet was supplemented with a fresh vegetable (carrot). Cholesterol and triglycerides were determined in the whole serum and in lipoprotein fractions. In rabbits fed standard diet ethanol caused a moderate elevation of VLDL cholesterol and triglyceride and LDL cholesterol levels. In animals on high-cholesterol diet cholesterol and triglyceride concentrations in these fractions were very high. Simultaneous consumption of large amounts of cholesterol and of ethanol resulted in a greater rise of cholesterol concentration in the whole serum and in VLDL and LDL fraction than did high-cholesterol diet alone. Addition of carrot caused a pronounced reduction of serum cholesterol concentration in animals fed all kinds of diets. The reduction concerned mainly VLDL.

Animals↗
PubMedDate not supplied

[Granulocyte adherence in advanced Hodgkin's disease and its dependence on antiproliferative drugs used].

In 36 patients with stage III and IV of Hodgkin's disease granulocyte adherence was investigated. The determinations were carried out in minicolumns filled with nylon fibres. In 13 patients the determinations were repeated 2, 4, 6, 12 and 24 hours after administration of vinblastine and cyclophosphamide. In all patients granulocyte adherence was reduced, by 54.1 +/- 3.5% on the average. The values of adherence in the control group were of the order of 83.7 +/- +/- 4.7%. Administration of antiproliferative drugs reduced further the adherence. The lowest value of it was observed 6 hours after vinblastine and cyclophosphamide administration. After that time the adherence increased gradually reaching the initial level 24 hours after administration of antiproliferative agents. These results suggest that reduced adherence of granulocytes may be an additional cause, besides abnormal immunological reactions, of the impairment of the resistance mechanisms of the organism in advanced stages of Hodgkin's disease.

Adult↗
PubMedDate not supplied

Apolipoproteins C in very low density lipoproteins (VLDL) in idiopathic hyperlipoproteinaemia.

The purpose of this study was an assessment of the relative proportions of apolipoprotein C subfractions in the VLDL fraction, which could contribute to explaining the development of metabolic disturbances in hyper-pre-beta-lipoproteinaemias. The study was carried out in 21 patients with hyperlipoproteinaemia type IIa, IIb, III, IV and in 8 subjects without lipid metabolism disturbances. Apolipoproteins C were separated in disc electrophoresis on polyacrylamide gel (PAGE) with added 8-M of urea. After the electrophoresis three main subfractions were obtained and were identified according to their electrophoretic mobility as Apo CII, Apo CIII1 and Apo CIII2. The relative per cent proportions of apolipoproteins C were determined by densitometry. A correlation was found between triglyceride concentration in the VLDL fraction and the relative amounts of Apo CII and Apo CIII1. Moreover, a raised ratio of the lipoprotein lipase inhibitor (Apo CIII1) to the activator of this enzyme (Apo CII) was demonstrated in hyperlipoproteinaemias type IIb, III and IV, which may suggest decreased susceptibility of these lipoproteins to the action of lipoprotein lipase.

Apolipoproteins↗