Biomedical subjects

G Ohsawa

Publications and source records attributed to G Ohsawa.

5 recordsLinked to original sources

PubMed1998

Crescentic glomerulonephritis developing 3 months after autologous peripheral blood stem cell transplantation for non-Hodgkin's lymphoma.

A 64-year-old woman underwent an ileocecal resection for ileus. The specimen revealed a diffuse large B cell lymphoma. The diagnosis was stage IIA non-Hodgkin's lymphoma. She received chemotherapy with the CHOP-etoposide regimen, resulting in partial remission. High-dose etoposide was used for PBSC mobilization before auto-PBSCT. Conditioning was ranimustine, carboplatin, etoposide and cyclophosphamide. Her renal function deteriorated gradually, starting 3 months post-PBSCT. Eight months post-transplant, serum creatine concentration was 7.1 mg/dl, and BUN was 59.2 mg/dl. Her hemoglobin concentration decreased to 5.3 g/dl, with no evidence of hemolysis. Renal biopsy revealed fibrous crescent formations in glomeruli, and mononuclear cell infiltration in interstitial spaces. Renal injury in this patient differs from BMT nephropathy, which is similar to hemolytic uremic syndrome, and represents another type of late renal injury after PBSCT.

Female↗

PubMed1998

[Investigation of the influence of hemodialysis membranes and factors associated with hemodialysis on serum (1-->3)-beta-D-glucan].

The measurement of serum (1-->3)-beta-D-glucan (beta-glucan) is commonly used in the clinical stage. Although it has been considered very useful in the early diagnosis of deep mycosis, it is influenced by several factors, one of which is hemodialysis. Therefore, we decided to investigate clinically the influence of hemodialysis membranes on serum beta-glucan. Plasma samples were collected from 26 patients receiving hemodialysis in Kawasaki Medical School Hospital. The kinds of hemodialysis membranes examined were four synthetic polymers and three natural polymers. We also measured beta-glucan in five washing liquids used in the hemodialysis circuit, five dialyzates, two sodium heparin and two filling liquids for wet type dialyzers to investigate the influence of other factors associated with hemodialysis on serum beta-glucan. Elevations of beta-glucan were observed in some cases treated by hemodialysis apparatus using a saponified cellulose acetate membrane. But, in all cases treated by hemodialysis using a cellulose triacetate membrane, slight elevations of beta-glucan were observed. No significant increases in beta-glucan after hemodialysis were observed in the cases treated by hemodialysis using a synthetic polymer membrane. Elevations of beta-glucan is sodium heparin and in one of the filling liquids for the wet type dialyzer were observed. We, therefore, concluded that there are some other factors having an effect on serum beta-glucan besides the hemodialysis membrane in the patients treated by hemodialysis.

Adolescent↗

PubMed1994

Polyclonal human T-cell activation by silicate in vitro.

Silica (SiO2) or related substances such as silicone ([-R2Si-O-]n), which is used in plastic surgery, or asbestos (e.g. chrysotile; 3MgO.2SiO2.H2O) have 'adjuvant effects'. In a study of scleroderma patients in Germany more than 78% had experienced exposure to silicate dust. T-cell receptor (TcR) V beta gene analysis on CD4- CD8- double-negative alpha beta T cells from scleroderma patients, using polymerase chain reaction (PCR), showed that certain V beta genes, V beta 5, V beta 7 and V beta 17, were predominantly expressed in the cells. We found that certain V beta repertoires, V beta 5.3 and V beta 6.7, were predominantly expressed on fractionated T cells with a high Ca2+ level that had been stimulated by chrysotile in vitro. The intracellular Ca2+ level in human peripheral blood mononuclear cells (PBMC) increased after incubation with silica or chrysotile. Interleukin-2 (IL-2) release from PMBC also rose significantly with chrysotile stimulation, but no change was observed when major histocompatibility complex (MHC) class II DP/DR positive cells were depleted. Therefore, our results support the possibility that silicate acts as a superantigen.

Adult↗

PubMed1991

[Neurosurgical management of dialyzed patients].

Cerebrovascular disease is a lethal complication for patients with renal failure because of the hemostatic disturbance and equivocality about management of the central nervous system. Appropriate management of the renal failure in neurosurgical patients was considered on the basis of experience. Hemodialysis (HD), which is the most common dialysis method, has a serious disadvantage: the elevation of intracranial pressure during dialysis due to the "disequilibrium syndrome." It is important to stabilize the serum osmolarity during dialysis in order to prevent the disequilibrium syndrome. From this point of view, continuous ambulatory peritoneal dialysis (CAPD) has great advantages; serum osmolarity is not rapidly changed and no anticoagulants are required during dialysis. CAPD is recommended as the first method of choice in neurosurgical management of renal failure patients. However, if a patient has to be maintained with HD because of a history of laparotomy or peritonitis, it is essential to keep the serum osmolarity as stable as possible using the extracorporeal ultrafiltration method, hypernatremic HD, bicarbonate HD, and intravenous administration of glycerol.

Adult↗

PubMedDate not supplied

Confirmation of dialysate leakage by intraperitoneal administration of radioactive colloid.

A 29-year-old man who had been on continuous ambulatory peritoneal dialysis (CAPD) was admitted to our hospital because of pleural effusion. Radioactive colloid studies with intraperitoneal administration revealed a pleuroperitoneal communication. After pleurodesis by the administration of fibrin glue and antiplasmin into the thoracic cavity, the pleural effusion did not reaccumulate and there was no evidence of pleuroperitoneal communication by radioactive colloid study.

Adult↗