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G Ordóñez

Publications and source records attributed to G Ordóñez.

5 recordsLinked to original sources
PubMed2005

Retinoic acid increases tissue and plasma contents of nerve growth factor and prevents neuropathy in diabetic mice.

BACKGROUND: Decreased production of nerve growth factor (NGF) may contribute to diabetic neuropathy; however, exogenous administration of NGF induces only a modest benefit. Retinoic acid (RA) promotes the endogenous expression of nerve growth factor and its receptor. We studied the effects of RA on diabetic neuropathy in mice with streptozotocin-induced diabetes. MATERIAL AND METHODS: One hundred and twenty National Institutes of Health (NIH) albino mice randomly separated into three groups (A, n = 30; B, n = 30; C, n = 60). Diabetes mellitus was induced with streptozotocin in groups A and B. Animals from group A received a subcutaneous injection of 25 microl of mineral oil daily for 90 days, while those from group B received a subcutaneous injection of 20 mg kg(-1) of all trans RA. Animals from group C were taken as controls. At the end of the experiment, blood glucose and NGF levels (both in serum and sciatic nerve) were measured. Two behavioural tests were conducted in a blind fashion to detect abnormalities of thermal and nociceptive thresholds. RESULTS: Contents of NGF in healthy untreated mice were 1490 +/- 190 pg mg(-1) in nerve and 113 +/- 67 pg mg(-1) in serum; in diabetic untreated mice the values were 697 +/- 219 pg mL(-1) in nerve and 55 +/- 41 pg mL(-1) in serum; and in diabetic mice treated with RA the values were 2432 +/- 80 pg mL(-1) in nerve and 235 +/- 133 pg mg(-1) in serum (P < 0.002). Ultrastructural evidence of nerve regeneration and sensitivity tests improved in diabetic mice treated with RA as compared with nontreated diabetic mice. CONCLUSION: Our findings indicate that administration of RA increases serum and nerve contents of NGF in diabetic mice and suggest a potential therapeutic role for retinoic acid in diabetic patients.

Animals↗
PubMed2005

Viral genotyping of infectious bursal disease viruses isolated from the 2002 acute outbreak in Spain and comparison with previous isolates.

An infectious bursal disease (IBD) outbreak occurred in the east region of Spain in the spring of 2002 and rapidly spread thorough the whole country, although proper vaccination programs were applied. In this report, 33 infectious bursal disease viruses (IBDVs) isolated from this outbreak were characterized by nucleotide sequencing of the VP2 gene hypervariable region and were compared with reference IBD strains and the 1990s Spanish IBDVs in order to determine possible emergence of IBDV isolates with modified antigenic or virulent properties. Moreover, histopathologic and immunohistochemical studies of those cases where bursal tissues were available were carried out. Of the 33 isolates, 23 were identified as very virulent IBDVs (vvIBDVs), whereas the other 10 isolates were classified as attenuated or intermediate virulence classical strains and could possibly be IBDV live vaccine strains used in the immunization of these chickens. Results of this study indicate that wIBDV isolates from the 2002 Spanish outbreak are closely related with those from the 1990s outbreak. However, acute IBD cases have not been reported in Spain during these 10 yr. Genetic, management, and environmental factors likely related with IBD reemergence in Spain are discussed. Moreover, our results indicate that good correlation exists between the IBDV subtype present in the field and the degree of lesions in bursa tissue, as well as the immunohistochemistry staining.

Amino Acid Sequence↗
PubMed1990

Diprosopia in a cat.

A diprosopic cat is described. In the head, two snouts, three eyes and two pinnae were present. The mandible was single and immobile because labial skin of both upper lips and single lower lip was partly fused. Superimposition of upper and lower dental arches was impossible and the mouths remained permanently open. Two incomplete oral cavities were present and the two tongues were joined at their base. The brain was duplicated in part. In the cranium only occipital and temporal bones were normal, the basisphenoid was bifurcated and the remaining bones were duplicated. Embryological mechanisms are discussed.

Animals↗
PubMedDate not supplied

[Usefulness and safety of early stress test after coronary angioplasty].

Exercise stress test before hospital discharge in patients treated with percutaneous transluminal coronary angioplasty (PTCA) has been used from the beginning of this technique to know the immediate clinical results, although there is not enough and clear information about the prognostic value and the possible complications related to this procedure. The objective of this study was to determine the safety and prognostic value of an early stress test post PTCA in our Hospital. In a retrospective fashion the charts of consecutive patients treated with PTCA were reviewed, with selection of those in whom a stress test was done in the first 10 days after the procedure. From 224 consecutive patients with PTCA, 83 (34%) had pre discharge stress test (mean age 52.8 +/- 9.7 years, 84% were male). In all 83, the PTCA procedure was successful in 112 lesions. 78% were treated for one vessel, and 19 and 2.5% in two or three vessels respectively. Stress tests were done at 5.1 days (range 2 to 10) and in 27 (32%) the tests were done in the first 3 days. There were no local vascular complications at the puncture site, and none of the patients had angina or myocardial infarction related to the test. There were 4 positive early tests (5%). Of them, in two cases there was incomplete revascularization. In all four cases, the thallium perfusion test was positive, and restenosis was seen in three of the cases at 3 to 6 months. The remaining patient is in class I, with minor inferolateral ischemia and the treating physician decided not to recatheterized her. Eight patients (9%) with negative pre discharge stress test had a positive one at control. Of them, three had angiographic control, and two had restenosis. It can be concluded that an early stress test post PTCA is safe, and can be done before discharge, with the possible exception of those with high risk of acute reocclusion. A positive result do not implicate immediate recatheterization, but can be associated with a major probability of late persistent myocardial ischemia and restenosis.

Adult↗