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Biomedical subjects

G Osorio

Publications and source records attributed to G Osorio.

At least 19 recordsLinked to original sources
PubMed2001

[Search for the spirochete Borrelia burgdorferi sensu lato by polymerase chain reaction in wild Chilean ticks].

BACKGROUND: Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection. AIM: To detect the presence of B burgdorferi in Chilean ticks. MATERIAL AND METHODS: A total of 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions. RESULTS: B burgdorferi was not detected in the studied ticks. CONCLUSIONS: Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative.

Animals↗
PubMed1999

Hyperbaric oxygen therapy for hepatic artery thrombosis after liver transplantation in children.

Early hepatic artery thrombosis (HAT) after pediatric orthotopic liver transplantation (OLT) can cause significant morbidity and mortality, leading to liver failure or septic complications requiring urgent retransplantation. Experimental evidence that hyperbaric oxygen (HBO) may ameliorate hepatic ischemic-reperfusion injury led to this study of HBO in pediatric liver transplant recipients who developed HAT. Children undergoing OLT under primary tacrolimus immunosuppression and University of Wisconsin organ preservation between August 1, 1989, and December 31, 1998, who developed HAT were the basis for this study. Patients who developed HAT between March 1, 1994, and December 31, 1998, were treated with HBO therapy until signs of ischemia resolved (absence of fever, normalizing liver injury test results) or for 2 weeks. The pediatric OLTs performed from August 1, 1989, to February 28, 1994, who developed HAT served as a control group. Primary outcome measures were survival, retransplantation rate, time to retransplantation, incidence of hepatic gangrene, and days to collateral formation. Three hundred seventy-five consecutive pediatric patients underwent 416 OLTs between August 1, 1989, and December 31, 1998. Thirty-one patients (7.5%) developed HAT at a mean time of 8.2 days (range, 1 to 52 days) post-OLT. In 17 patients, HBO treatment was begun within 24 hours of HAT or immediately after the revascularization attempt and performed twice daily for 90 minutes at 2.4 atmospheres pressure. Fourteen patients were treated without HBO. None of the HBO-treated patients developed hepatic gangrene. Eight HBO patients (47%) were bridged to retransplantation at a mean time of 157 days (range, 3 to 952 days) after initial OLT and all survived. Mean time to retransplant in the control group was 12.7 days (range, 1 to 64 days). HBO was well tolerated without significant complications. Although there was no significant difference in survival or retransplantation rates, HBO significantly delayed retransplantation, potentially by hastening the development of hepatic artery collaterals.

Arterial Occlusive Diseases↗
PubMed1996

Phosphorylation of GroEL, DnaK and other proteins from Thiobacillus ferrooxidans grown under different conditions.

The levels of phosphorylation of the chaperones DnaK and GroEL and other proteins varied when cells of Thiobacillus ferrooxidans were subjected to phosphate starvation. The phosphorylated amino acid of GroEL was found to be threonine. Our results show that not only heat shock, but also a nutrient starvation stress leads to phosphorylation of chaperones and, in addition, support the possible role of phosphorylation of these proteins in the sensing and regulation of stress responses in bacteria.

Bacterial Proteins↗
PubMed1991

[Treatment of Hodgkin's disease. National Protocol of Antineoplastic Drugs: preliminary report].

We report preliminary results of treatment of Hodgkin disease according to the National Protocol on Antineoplastic Drugs. 37 males and 22 females with a median age of 34 years (range 16 to 76) were treated. Patients with stages I or II received radiotherapy alone or chemotherapy (C-MOPP) followed by radiotherapy. Patients with stages III or IV received radiotherapy and chemotherapy or alternating courses of C-MOPP and ABVD. Complete remission was observed in 74% of 39 patients completing full therapy. Complete remission was more common in patients with nodular sclerosis without B symptoms and under 45 years of age. Actuarial survival at 22 months was 79%, significantly higher for patients with nodular sclerosis compared to patients with lymphocytic depletion. Different treatments in patients at stages I and II or III and IV gave comparable results. Complications included infection with the Chickenpox-Zoster virus, pneumonia and fever of unknown origin. Mortality was associated to older age, complications of treatment or failure to comply with therapy.

Actuarial Analysis↗
PubMed1991

[Malignant lymphomas. Preliminary report of the National Protocol of Antineoplastic Drugs].

The first results of the management of adult non-Hodgkin lymphoma according to the National Protocol are reported. 164 pts (71 female and 93 male), aged 50 (range 15 to 79 years) were included. 89 pts had lymphomas with intermediate, 34 with low and 24 with high grade malignancy. 65% had advanced stages (III and IV). In the low grade malignancy group 38% were in complete remission and actuarial survival at 20 months was 80%. In the intermediate grade group, stages I and II the corresponding values were 79 and 85%; in the intermediate grade stages III and IV, 58 and 69%, and in the high grade group 61 and 77%. Bone marrow toxicity and infections were the main complications. 32 pts have died, 10 of them with drug toxicity complications.

Adolescent↗
PubMed1990

[Treatment of acute lymphoblastic leukemia in adults: preliminary report of the national protocol on antineoplastic drugs].

We report preliminary results from a national protocol for treatment of acute lymphoblastic leukemia. 44 patients, 15 females and 29 males with age range 15 to 65 years (median 27) were treated and followed for 22 months. Complete remission was observed in 33 (77%) of 43 patients with a known induction result. Remission was 100%, 86% and 61% for low (3), medium or high risk patients (p = 0.03). Complete remission occurred more often in patients with WBC less than 35,000 or granulocyte counts greater than 100. Actuarial survival rate at 22 months was 60% overall and 100%, 64% and 50% for the low, intermediate and high risk groups, respectively (NS). Death occurred in 16 patients, 5 during induction, 3 during complete remission, 6 during relapse and 2 after induction failure. Infections were the main cause of death in all groups.

Actuarial Analysis↗
PubMed1990

[Treatment of acute non-lymphoblastic leukemia in adults. Preliminary report from the national protocol on antineoplastic drugs].

After a follow up of 22 months we report results of treatment on 44 patients (22 males), aged 16 to 63 years, with acute non lymphoblastic leukemia. Complete remission was obtained in 22 patients. This was significantly more frequent in patients under 40 years of age with white cell counts under 35,000 and without metabolic complications nor disseminated intravascular coagulation before treatment. Delaying chemotherapy for 5 days after diagnosis was also associated to a better prognosis. Overall actuarial survival rate was 37% at 15 months, 55% for those experiencing a first remission. A total of 25 patients [corrected] have died, 15 during induction of therapy, 7 after complete remission and 3 after failure of induction. Infections are the main cause of death during induction, with a high lethality for sepsis (33%) and pneumonia (40%).

Actuarial Analysis↗
PubMed1989

[Treatment of Hodgkin's lymphoma].

From November 1976 until September 1987, 59 patients with Hodgkin's disease have been admitted and treated with radiotherapy alone or radiotherapy plus elective chemotherapy after been staged according to Stanford's guidelines and following the Ann Arbor's classification. 33 patients were staged I or II, and 26 as stage III or IV. Patients in earlier stages received preferentially radiotherapy alone and those in more advanced stages received radio and chemotherapy. After a median follow-up of 39 months, 100% of patients in stage I, 81.5% of patients in stages II and III A and 45% of patients in stages III B and IV were alive and well. The relapse free survival for patients in stages II and III A was 48.4%, rising to 81.5% after rescue. The use of elective chemotherapy made no difference in survival for stages II B and III A.

Adolescent↗
PubMed1976

Differences among cattle and farms as related to beef cow reproduction in the eastern plains of Colombia.

In the tropical savannas of Colombia, the Llanos Orientales, a field survey of 40 beef herds leased by farmers through a cooperative indicated that these herds had mean annual calving rates of 52 and 55 per cent in the Plains and Piedmont zones, respectively. Within zones, very large differences in reproductive rates were found between herds. The differences seemed to be associated with the soils and climates of the areas, condition and weight of cows and breed of cow. Only 9 to 13 per cent of nursing cows were found to be pregnant. Conception was seasonal, occurring mostly in the dry and early rainy seasons. Control of the time of weaning of calves, routine mineral supplementation and improved pasture management appeared to offer immediate possibilities for economically improving output of calves.

Animal Feed↗