[The microevolution of anthropophilic prokaryotes as a factor altering the activity of epidemic processes. The microevolution of pathogenicity].
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Biomedical subjects
Publications and source records attributed to G P Kalina.
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The multidimensional niche in Gause's law includes, with respect to prokaryotes, the following factors: the alimentary factor, the symbiosis-antagonism complex, the respiratory function, the concentration of associants, mobility. Cultivation in U-shaped tubes is the optimum method for the detection of spatial separation on account of such factors as respiration and mobility.
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The author advances a hypothesis stating that a species disappearing as a result of new conditions evolving in a colonized animal or human body, unfavorable for the existence of this species, does not become extinct, but reverts into newly developing species due to the loss of its properties and acquiring new properties corresponding to new conditions. Thus the genus is preserved through the loss of species characteristics by individual organisms. The advanced hypothesis is substantiated by the analysis of the relevant processes observed in cases of infectious keratoconjunctivitis in humans and cattle, caused by bacteria of the genus Moraxella. The data indicating the possibility of such reversion in the genera Neisseria and Bordetella are presented.
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Various microbial species, embraced by the genera, have been found to correspond to their phylogeny by the types of nutrition and the levels of pathogenicity. A relationship between the types of nutrition and the levels of parasitism and pathogenicity has been revealed. The results of analysis make it possible to assume, with a great degree of probability, the existence of new species (subspecies, variants) in the environment and/or as agents of diseases of unclear etiology.
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To determine the gelatinase activity of Gram-negative nonfermenting microorganisms, a double-layer medium containing 2% of agar in the lower layer and 10-20% of gelatin in the upper layer has been developed. The medium ensures free access of atmospheric oxygen, possibility of rapid (24-42 h) determination of gelatinase activity at 22-25 degrees C, efficacy of the test. simultaneous determination of gelatin hydrolysis by 8-12 strains in one dish.