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G Palestro

Publications and source records attributed to G Palestro.

119 records · Page 7Linked to original sources
PubMedDate not supplied

Expression of the CD31 antigen in normal B-cells and non Hodgkin's lymphomas.

The role cell adhesion molecules play in the biological and clinical behaviour of non Hodgkin's lymphomas (NHL) has been reported in several studies. This study reports the findings on B-cells taken from various healthy control tissues and compared them to B-cells from 83 malignant B-lymphomas, that had been classified according to the WHO classification. Flow cytometry was used to investigate the surface expression of CD31, an adhesion molecule involved in B-cell development and vascular adhesion mechanisms. Quantification of the fluorescence signals showed specific patterns of CD31 expression on normal B-cell subpopulations and different NHL groups. Our results demonstrate that CD31 expression is modulated during the differentiation process in normal B-cells, high in pre-B-I cells, low in pre-B-II precursors, intermediate in the mature B-cell subpopulations or, depending on the functional state absent in activated follicular centre cells, present in pre- and post- germinal centre cells. When the CD31 expression is evaluated as fluorescence intensity in NHL, it reveals a heterogeneous pattern related to histogenetic derivation (high in small lymphocytic lymphoma, low in follicular lymphoma, intermediate in marginal zone and large cell lymphomas). These observations suggest that CD31 might well play a critical role in the ontogeny and physiology of B-lymphocytes. Therefore, on the basis of these observations we propose the CD31 molecule as an interesting additional useful parameter to be used for the differential diagnosis of NHL and hypothese that it has a pathophysiologic role in NHL evolution.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Comparative morphological study of the structure of the normal and arteriosclerotic internal mammary, coronary, and renal artery wall].

Morphological comparative study of the normal anatomy of the internal mammary artery, coronary artery and renal artery, and their atherosclerotic alterations. We report in this paper the comparative results of a morphological and morphometrical study of the normal anatomy of the Left Coronary Artery (interventricular descending branch) (LCA), Internal Mammary Artery (IMA) and Renal Artery (RA) and their atherosclerotic alterations in 27 unselected people of both sexes aging from 19 to 76 years (average 59 +/- 14.3). Sections from three different segments of each vessel were examined by measuring the thickness of the intima and media calculated at the maximum intimal thickening. The lumen was also measured at the level of its highest pathological reduction; the same measurements were carried out on sections free from atherosclerotic damage. Then the intima to media ratio was assumed as major indicator of atherosclerosis involvement according to the literature. All these parameters were statistically analysed. With these procedures we found that LCA displayed more severely atherosclerotic changes than IMA. The degree of damage in RA fell in between when compared to the other arteries. The intima to media ratio average was 0.061 mm. (+/- 0.084) in I.M.A.; 0.882 mm. (+/- 0.753) in L.C.A. and 0.272 (+/- 0.315) in RA with statistically significant differences between IMA and LCA (p less than 0.001) and between IMA and RA (p less than 0.001). In the lesion free sections intima to media ratios were 0.178 (+/- 0.90) in LCA, 0.053 (+/- 0.28) in IMA and 0.082 (+/- 0.127) in RA. The difference was statistically significative between LCA and IMA, but not between RA and IMA.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMedDate not supplied

[Incidence of atherosclerosis of the internal mammary artery compared with the coronary artery. A study of 22 non-selected autopsy cases].

In the present work the examination and comparison of the degree of atherosclerotic involvement of the left coronary artery (CDA) and internal mammary artery (AMI) was performed on 22 unselected subjects at necroscopy examination. The maximum age was 77 and the minimal was 25 (average 62.59 +/- 2.47); 13 were male and 9 female. We macroscopically and microscopically examined three sections obtained from three different segments for each vessel. First we macroscopically evaluated the degree of narrowing of the lumen giving a score (+ to +). (Tab. II). Then the sections, 6 micron thick, stained with Haematoxylin-eosine, Alcian blue, PAS and Van Gieson methods were accurately examined at microscope with a graduated eyepiece. The lumen, the intima and the media were measured at the level of maximum thickness of the intima. Then the thickness of intima and the intima to media ratio, accordingly to the literature, were assumed as major indicators of atherosclerosis involvement. The intima to media ratio average was 5.05 (+/- 5.81) in the CDA vs. 0.44 (+/- 0.29) in the AMI (p less than 0.001). The intima in the CDA was 0.86 mm (+/- 0.76) thick (average) vs. 0.09 mm (+/- 0.07) in the AMI (p less than 0.001). These results show a great difference in atherosclerosis involvement which results to be ten times greater in CDA than in AMI in non selected people. Almost the same result is obtained by the measurement of the intima. On the contrary no detectable difference was found between the media of the two vessels.

Adult↗
PubMedDate not supplied

[Immunological changes in diabetes mellitus. Study of lymphocyte populations].

In view of some data of the literature suggesting that immunological changes could play a role in the pathogensis of diabetes mellitus and of its complications, we carried out a study of the lymphocyte populations of 44 diabetic subjects by means of E, EA, EAC rosettes and membrane immunofluorescence. A quarter of the patients had decreased E rosettes: all of these had long-lasting, insulin-dependent, poorly controlled diabetes. Some of these patients had also increased membrane IgG globulins. In 5 patients with recent onset, insulin-independent diabetes, E rosettes were increased. No significant changes of EA and EAC rosettes were observed. These findings suggest that changes of lymphocyte populations do not play any role in the pathogenesis of diabetes, while these may be the consequence of the metabolic disorder and these may favour the onset of complications of the disease.

Adult↗
PubMedDate not supplied

[Immunopathological characteristics of the lymphocytes of chronic lymphocytic leukemia, fractionated on gradients of various densities].

Spontaneous rosettes and membrane immunofluorescence for the various classes of heavy and light immune globulin have been determined on lymphocyte subfractions obtained from the blood of 6 normal subjects and 16 patients with CLL by separation on gradients of various density. Whereas in the normal subject lymphocyte immunological features proved similar in all fractions, in CLL they presented significant differences from fraction to fraction, with the presence only in certain fractions of the lymphocyte peak with monoclonal membrane immune globulin and without peak coincidence for IgD and IgM, probably as a result of changes in the physico-chemical features of the lymphocytes occurring during maturation. All fractions presented a sizeable complement of lymphocytes not forming rosettes and without demonstrable membrane immune globulins. The correlation between the behaviour of membrane labels and lymphocyte morphology, stage, duration and course of the disease has been analysed. The variability in lymphocyte subfraction characteristics separable at different densities may explain discrepancies of findings in researches carried out in lymphocytes that separate only at gradient of density 1077.

Aged↗
PubMedDate not supplied

Identification of granulocyte-macrophage colony stimulating factor receptor mRNA by non-isotopic in situ hybridization in bone marrow biopsies.

BACKGROUND: In the last few years many studies have been published on GM-CSF receptors, focusing on molecular structure, function and distribution. Nevertheless, protocols for detecting GM-CSF receptors on formalin-fixed paraffin-embedded histological sections, to our knowledge, have not been described. METHODS: A method based on non-isotopic in situ hybridization (ISH) using a 21-base antisense DNA oligoprobe whose 3'-end was labeled with digoxigenin 11-dUTP was devised. The probe was applied on 20 routinely processed bone marrow trephine biopsies which were considered as positive controls. RESULTS: The hybridization signal was seen in myeloid cells, erythroid progenitors and rare megakaryocytes. CONCLUSIONS: Non-isotopic ISH represents an alternative to current methodologies for the assessment of GM-CSF receptor expression; since it is suitable for routinely processed samples, it can be regarded as a helpful tool for diagnostic determination of GM-CSF receptors in tumors from patients receiving GM-CSF and for retrospective studies on archival material.

Base Sequence↗
PubMedDate not supplied

Significance of cell proliferation index in assessing histological prognostic categories in Hodgkin's disease. An immunohistochemical study with Ki67 and MIB-1 monoclonal antibodies.

BACKGROUND AND OBJECTIVE: In their review of the Rye histopathological classification of Hodgkin's disease, Bennett and coworkers have proposed that the nodular sclerosis (NS) type should be divided into two diagnostic categories on the basis of their clinical behaviour. In order to evaluate whether the proliferative activity of HD cells might correlate with histology in NS subtypes, we reviewed and re-evaluated cryostat and paraffin-embedded sections from 80 cases sent to our centre from 1986 to 1991. METHODS: In the present study, we investigated the growth cell fraction of 53 cases of Hodgkin's disease with nodular sclerosis by using Ki67 and MIB1 monoclonal antibodies to determine whether proliferative activity is associated with different pathological subtypes and prognostic categories. Eight cases with an interfollicular pattern and 19 with mixed cellularity were also investigated. The results in each group were compared to the others. RESULTS: The values of Ki67 and MIB1 were highly correlated (r = 0.88). In Hodgkin's disease with nodular sclerosis, two groups with significantly different growth fractions were morphologically identified: one with lymphocyte predominance and mixed cellularity subtypes, another composed of cases with variously extensive lymphocyte depletion. The figures were compared with those of interfollicular subtype, which fell into the first group, and of mixed cellularity type, in which the proliferative cell activity was significantly higher than in the second nodular sclerosis group. In all cases, Reed-Sternberg and Hodgkin cells accounted for the majority of the cell growth fraction, although a variable percentage of T-lymphocytes were also Ki67- or MIB1-positive. Taking the median value (15%) of MIB1 positive cells as a cut-off, a significant correlation (p = 0.05) was observed between MIB1 positivity and bulky disease, and a good trend (but not a significant relationship) between MIB1 and overall survival, disease-free survival, staging and the clinical response to therapy. INTERPRETATION AND CONCLUSIONS: Assessment of the growth cell fraction in Hodgkin's disease with different nodular sclerosis patterns provides biological support for the morphological reclassification of their degree of malignancy into two main groups with different impacts on the clinical parameters and a possible relation with the outcome of treatment.

Antibodies, Monoclonal↗